Probe, primer and kit for detecting talent gene

A kit and gene technology, applied in the field of molecular biology, can solve the problems of cumbersome detection steps, low sensitivity and poor specificity, and achieve the effects of fast detection speed, high detection sensitivity and simple operation.

Pending Publication Date: 2017-12-08
上海闪锦生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the methods for detecting congenital genes are DNA direct sequencing method, chip method or dye method, etc. However, direct sequencing method, chip method, and dye method have low sensitivity and poor specificity, only 20-30%, and the detection steps are cumbersome and inefficient. At least It takes 2-3 days to get the results; and if the genomic DNA content of the sample is scarce, the direct sequencing method will lead to a large number of missed detections and false negatives, so a high-sensitivity and high-specificity detection method is especially needed to realize the detection of gene mutations detection

Method used

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  • Probe, primer and kit for detecting talent gene

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Effect test

Embodiment 1

[0037]1. Detection of the present invention with genetically engineered recombinant plasmids, with the rs4680 mutant as the detection object. Plasmids with rs4680 mutation gene constructed by genetic engineering were used as detection objects. Plasmid pCOMTM was a plasmid with rs4680 mutation; plasmid pCOMTW was a plasmid with wild-type rs4680 gene. These plasmids were dissolved in TE buffer (10mM Tris-HCl, 1mMEDTA, PH=8.0), the mass and concentration were measured by UV spectrophotometer, the plasmid copy number was calculated, and 10000, 1000, 100, 10 copies were obtained by gradient dilution with TE buffer / ul of pCOMTM and pCOMTW plasmid solutions, and then used as templates for PCR reactions.

[0038] 2. Synthesis of COMT-specific primers and probes: According to the mutation site of the human COMT gene (rs4680), design 2 probes, 5' end labeled FAM (green fluorescence) or VIC (yellow fluorescence), 3' end labeled MGB The quenching group was synthesized and labeled by Sha...

Embodiment 2

[0063] 1. The present invention is detected by genetic engineering recombinant plasmids, and the rs1456031 mutant is used as the detection object. Plasmids with rs1456031 mutation gene constructed by genetic engineering were used as detection objects. Plasmid pFOXP2M was a plasmid with rs1456031 mutation; plasmid pFOXP2W was a plasmid with wild-type rs1456031 gene. These plasmids were dissolved in TE buffer (10mM Tris-HCl, 1mMEDTA, PH=8.0), the mass and concentration were measured by UV spectrophotometer, the plasmid copy number was calculated, and 10000, 1000, 100, 10 copies were obtained by gradient dilution with TE buffer / ul of the pFOXP2M and pFOXP2W plasmid solutions were then used as templates for PCR reactions.

[0064] 2. Synthesis of FOXP2-specific primers and probes: According to the mutation site of human FOXP2 gene (rs1456031), design 2 probes, 5' end labeled with FAM (green fluorescence) or VIC (yellow fluorescence), 3' end labeled with MGB The quenching group w...

Embodiment 3

[0089] 1, detect the sensitivity of the present invention with genetic engineering recombination plasmid, the plasmid with COMT mutation gene constructed through genetic engineering is as detection object, and plasmid pCOMTM is the plasmid with RS4680 mutation; Plasmid pCOMTW is the plasmid with wild-type COMT gene . These plasmids were dissolved in TE buffer (10mMTris-HCl, 1mMEDTA, PH=8.0), the mass and concentration were measured by UV spectrophotometer, the plasmid copy number was calculated, and 100 copies / ul pCOMTM and pCOMTW plasmids were obtained by diluting with TE buffer solution, which then served as a template for the PCR reaction.

[0090] 2. Synthesis of COMT-specific primers and probes: According to the mutation site of the human COMT gene, design 2 probes, the 5' end is labeled with FAM (green fluorescence) or VIC (yellow fluorescence), and the 3' end is labeled with MGB quencher group; synthesized and labeled by Shanghai Shining Molecular Biotechnology Co., Lt...

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Abstract

The invention relates to a probe, a primer and a kit for detecting a talent gene, and discloses a probe, a primer and a kit for detecting a logic intelligence gene and a language intelligence gene in the talent gene. According to the probe, the primer and the kit, the probe and the primer are designed for an rs4680 site of a COMT (catechol-O-methyltransferase) gene; the probe and the primer are designed for an rs1456031 site of an FOXP2 (forkhead box P2) gene; a real-time fluorescent PCR (polymerase chain reaction) system is established; rs4680 and rs1456031 mutation forms can be detected; the detection sensitivity is high; mutation of 100-500cp can be detected out; personal genome DNA (deoxyribonucleic acid) from sources such as swabs, fresh and refrigerated whole blood, plasma and body fluids can be directly adopted; the operation is simple; the detection is cheap; a clinical application scope is wide; the detection specificity is high; the detection speed is high; and a detection process only requires 80min.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to probes, primers and kits for detecting logic intelligence genes and language intelligence genes in innate genes. Background technique [0002] A talent is a gift, a growth characteristic that comes before you grow up. There may also be natural abilities or natural obsessions (great enthusiasm) in certain things or fields, so that they can grow faster than others with the same experience or even no experience. , and has its uniqueness and particularity. If the person's growth potential and possibility have been expressed in his life, then he has the talent in this area. [0003] Every child is a little angel. They hid their wings when they came down to earth, and turned them into their talents, so every child is special and has talents waiting to be discovered, but not all parents have discerning eyes Bole often fails to see the talent of the child, so he has to enr...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q2561/101C12Q2563/107C12Q2531/113
Inventor 罗益华吴丽萍陈世杰
Owner 上海闪锦生物科技有限公司
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