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Preparation method and application of photothermal antibacterial agent polypeptide/Au/Fe3O4

A technology of photothermal agent and decomposition method, which is applied in the direction of antibacterial drugs, resistance to vector-borne diseases, and medical preparations containing active ingredients, etc. It can solve problems such as harm, drug-resistant bacteria not working, and limit antibacterial infection. Achieve the effect of improving utilization rate, reducing damage and good targeted sterilization

Active Publication Date: 2017-12-12
兰州博林化工科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, with the extensive use of antibiotics, many bacteria have developed resistance to many antibiotics, making the treatment of bacterial infections more and more difficult
Currently, existing antibiotics do not work against some drug-resistant bacteria, and the development of new antibiotics requires a lot of time and cost
In addition, other antibacterial methods such as the use of bactericide chlorine, silver, strong oxidants, low-temperature plasma technology and photocatalytic degradation, etc., limit their application research in vivo (In vivo) antibacterial infection due to their own shortcomings and potential hazards

Method used

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  • Preparation method and application of photothermal antibacterial agent polypeptide/Au/Fe3O4

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of Fe 3 o 4 NPs: Iron ferric oxide nanoparticles were prepared by high-temperature reduction decomposition method, the specific steps are as follows: Weigh 0.706g iron acetylacetonate (Fe(acac) 3 ) into a three-necked flask, then measure 10mL of dibenzyl ether, 10mL of oleylamine, and 50mL of ethanol into the flask, and then stir at 110°C for 1h for dehydration. The temperature was then raised to 300 °C and stirring was continued for 2 h. Naturally cool to room temperature after the end, and then centrifuge at 8000rpm to obtain a black product, and use a strong magnet to wash the product three times with hexane, ethanol, and water. Then place it in an oven at 60°C for 12 hours.

[0029] (2) Preparation of Au / Fe 3 o 4 Core-shell composite: Weigh the Fe prepared in step 1 3 o 4 0.01 g of NPs was redispersed in 40 mL of distilled water in an ice bath, and then 0.6 mL of chloroauric acid (HAuCl 4 ) solution, while adding 0.2mL of sodium carbonate (Na...

Embodiment 2

[0033] Same as Example 1, only 1.2 and 2.4 mL added in step (2) of Example 1 were changed. i.e. final Fe 3 o 4 with HAuCl 4 The mass ratios are 5:1.5 and 5:6. The final sterilization rate is shown in Table 1. The results showed that with the increase of the amount of Au, the peptide / Au / Fe 3 o 4 The photothermal efficiency is improved accordingly, and the final sterilization rate is improved.

[0034] Table 1 The amount of different Au versus polypeptide / Au / Fe 3 o 4 The effect of the bactericidal efficiency of

[0035] Fe 3 o 4 with HAuCl 4 mass ratio

Embodiment 3

[0037] Same as Example 1, only change polypeptide / Au / Fe in Example 1 step (4) 3 o 4 The concentration is 200, 300μg / mL. The final photothermal sterilization rate is shown in Table 2. The results showed that with the peptide / Au / Fe 3 o 4 As the concentration increases, the final photothermal sterilization efficiency increases accordingly.

[0038] Table 2 Different polypeptides / Au / Fe 3 o 4 The effect of concentration on its bactericidal efficiency

[0039] Peptide / Au / Fe 3 o 4 Concentration (μg / mL)

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Abstract

The invention discloses a preparation method and application of a photothermal antibacterial agent polypeptide / Au / Fe3O4, and belongs to the field of preparation of materials. The preparation method mainly comprises the following steps: (1) preparing Fe3O4-NPs; (2) preparing a Au / Fe3O4 core-shell composite material; (3) preparing a peptide / Au / Fe3O4 core-shell composite material; and (4) performing photothermal sterilization. The Fe3O4 serves as a core, so that the composite material has good photothermal effect, can be recovered and reutilized easily through a strong magnet, greatly increases the utilization rate and saves energy. The outer layer has bacterial selectivity polypeptide, so the composite material can target bacteria well and reduce damage to the peripheral normal cells.

Description

technical field [0001] The present invention relates to a kind of preparation method and application of photothermal antibacterial, especially refer to ferric oxide (Fe 3 o 4 ), gold (Au) and polypeptide (polypeptide) are compounded to prepare polypeptide / gold / iron tetroxide (polypeptide / Au / Fe 3 o 4 ) nanocomposite material, and applied to photothermal sterilization under near-infrared (NIR) light irradiation, belonging to the field of material preparation. Background technique [0002] In recent years, with the extensive use of antibiotics, many bacteria have developed resistance to many antibiotics, which makes the treatment of bacterial infections more and more difficult. Currently, existing antibiotics do not work against some drug-resistant bacteria, and the development of new antibiotics requires a lot of time and cost. In addition, other antibacterial methods, such as the use of bactericide chlorine, silver, strong oxidants, low-temperature plasma technology and p...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/42A61K47/02A61P31/04
CPCA61K41/0052A61K47/02A61K47/42Y02A50/30
Inventor 刘磊张杰冯永海陈清玉董明东
Owner 兰州博林化工科技有限公司
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