Method for extracting high-purity alpha-linolenic acid by taking flaxseed as raw material

A flaxseed, high-purity technology, applied in chemical instruments and methods, pharmaceutical formulations, carboxylate preparation, etc., can solve the problem of only 70% high, achieve low production cost, good separation effect, and wide application value Effect

Inactive Publication Date: 2017-12-15
华子昂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for separating and purifying α-linolenic acid from flaxseed in the prior art mainly include: urea inclusion method, silver ion complexation method, column chromatography, molecular distillation method, and β-cyclodextrin inclusion method, such as the invention Patent CN102628003A discloses a method for extracting and purifying α-linolenic acid f

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting high-purity α-linolenic acid from linseed, comprising cold pressing, refining, dewaxing, ethyl esterification, urea inclusion, molecular distillation, and column chromatography, the steps of which are as follows:

[0023] 1) The linseed is mechanically subjected to cold pressing, refining, and dewaxing processes to obtain linseed oil;

[0024] 2) Mix the ethanol solution of 2.5% KOH and linseed oil in the reaction kettle evenly, heat the ethyl esterification reaction at 65°C for 3.5-4.5 hours; The ester layer and the water layer, the ester layer is distilled to remove n-hexane, and the mixed fatty acid is obtained;

[0025] 3) Reflux 40% urea ethanol solution and the mixed fatty acid prepared in step 2) at 55-65° C. for 30-40 minutes until the mixture is completely dissolved. After cooling down to room temperature, slowly cool down to 0-10°C in 4-6 hours. ℃, precipitate crystals, then filter, adjust the pH value of the filtrate to 2-3 with hydro...

Embodiment 2

[0030] According to the method of Example 1, ethyl α-linolenate with a purity of more than 95% was neutralized, washed, and vacuum dehydrated to prepare ethyl α-linolenate tablets by conventional tablet production technology.

Embodiment 3

[0032] A method for extracting high-purity α-linolenic acid from linseed, comprising cold pressing, refining, dewaxing, ethyl esterification, urea inclusion, molecular distillation, and column chromatography, the steps of which are as follows:

[0033] 1) The linseed is mechanically subjected to cold pressing, refining, and dewaxing processes to obtain linseed oil;

[0034] 2) Mix 4% KOH ethanol solution and linseed oil in a reaction kettle evenly, heat at 65°C for ethyl esterification reaction for 3.5-4.5 hours; The ester layer and the water layer, the ester layer is distilled to remove n-hexane, and the mixed fatty acid is obtained;

[0035] 3) Reflux 65% urea ethanol solution and the mixed fatty acid prepared in step 2) at 55-65° C. for 30-40 minutes until the mixture is completely dissolved. After cooling down to room temperature, slowly cool down to 0-10°C in 4-6 hours. ℃, precipitate crystals, then filter, adjust the pH value of the filtrate to 2-3 with hydrochloric aci...

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PUM

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Abstract

The invention discloses a method for extracting high-purity alpha-linolenic acid by taking flaxseed as a raw material and a prepared alpha-ethyl linolenate preparation. The method comprises the following steps: cold-pressing, refining and de-waxing the flaxseed, thereby acquiring flax seed oil; dissolving the flax seed oil in ethyl alcohol for performing esterification reaction, thereby acquiring a mixed fatty acid; cladding urea, slowly cooling, evaporating and concentrating, thereby acquiring mixed unsaturated fatty acid ethyl ester containing over 70% of alpha-ethyl linolenate; performing molecular distillation on the crude product of alpha-ethyl linolenate, thereby acquiring the alpha-ethyl linolenate in purity above 85%; and purifying the alpha-ethyl linolenate in purity above 85% through column chromatography, thereby acquiring the alpha-ethyl linolenate containing more than 95% of alpha-linolenic acid. The method disclosed by the invention has the advantages of simple process, low production cost, excellent separating effect, high purity and suitability for large-scale production. The alpha-ethyl linolenate preparation prepared according to the method disclosed by the invention is high in purity, is stable and reliable, is convenient for large-scale production and use and has high application value.

Description

Technical field: [0001] The invention belongs to the technical field of preparation of linolenic acid, and in particular relates to a method for extracting high-purity alpha-linolenic acid from flax seeds. Background technique: [0002] The main essential fatty acids are omega-3 polyunsaturated fatty acids, which are the core life substances that maintain the evolution of the human brain. In particular, α-linolenic acid, the parent of omega-3 polyunsaturated fatty acids, is an essential fatty acid for the human body, which cannot be synthesized by the human body itself and can only be ingested from the outside world. It is an essential substance for human health, known as "plant brain gold" and "blood nutrient". If the human body lacks omega-3 polyunsaturated fatty acids, it will lead to poor development of gray and white matter in the brain, reduced optic nerve conduction velocity, easy fatigue, and skin allergies and other symptoms. Moreover, a large number of research r...

Claims

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Application Information

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IPC IPC(8): C07C69/587C07C67/08C07C67/62C07C67/52C07C67/54C07C67/56A61K31/232A61P25/28A61P35/00A61P9/00A61P9/12A61P3/06A61P3/10
CPCC07C67/08C07C67/52C07C67/54C07C67/56C07C67/62C07C69/587
Inventor 华子昂刘松洁竹添孙宁孙小惠马贤
Owner 华子昂
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