Compound bacterium colony for de-coloring papermaking industrial wastewater and preparation method thereof

A technology for paper industry wastewater and compound flora, which is applied in the process of wastewater treatment, microorganism-based methods, biochemical equipment and methods, etc. lower problem

Inactive Publication Date: 2017-12-15
QINGDAO TECHNOLOGICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of treating industrial wastewater with ozone oxidation technology, there are still some deficiencies, specifically: (1) Ozone utilization rate is not high due to reasons such as ozone self-decomposition and dissolution uti

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Using 10 parts of Pantothenic acid bacillus, 10 parts of Bachybacterium wilterii, 20 parts of white rot fungi, 15 parts of Pseudomonas putida, 30 parts of Agrobacterium, 10 parts of Pseudomonas stutzeri, 30 parts of Enterobacter cloacae , inoculating the strains on the slant medium in the test tube, and culturing them at 30° C. for 3 days;

[0017] 2) The above slant culture was made into a bacterial suspension with sterile water, and then 15 parts of Pseudomonas putida, 30 parts of Agrobacterium, 10 parts of Pseudomonas stutzeri, and 30 parts of Enterobacter cloacae were inoculated into the production organism. Cultivate separately in surfactant medium, and cultivate at 30°C for 3 days; 10 parts of Bacillus panthenica, 10 parts of Brevibacterium wilt, and 20 parts of white rot fungi are inoculated into bacterial culture medium and cultured at 30°C 3 days;

[0018] 3) The microbial fermentation liquid in step 2) is mixed according to the wet weight ratio of the micr...

Embodiment 2

[0020] 1) Use 2 parts of Pantothenic acid bacillus, 10 parts of Brevibacterium wilterii, 30 parts of white rot fungi, 10 parts of Pseudomonas putida, 20 parts of Agrobacterium, 10 parts of Pseudomonas stutzeri, 20 parts of Enterobacter cloacae , inoculating the strains on the slant medium in the test tube, and culturing them at 25° C. for 7 days;

[0021] 2) The above slant culture was made into a bacterial suspension with sterile water, and then 10 parts of Pseudomonas putida, 20 parts of Agrobacterium, 10 parts of Pseudomonas stutzeri, and 20 parts of Enterobacter cloacae were inoculated into the production organism. Cultivate separately in surfactant medium, and cultivate at 25°C for 7 days; 2 parts of pantothenic acid bacillus, 10 parts of Brevibacterium wilt, and 30 parts of white rot fungi are inoculated into bacterial culture medium and cultured at 25°C 7 days;

[0022] 3) The microbial fermentation liquid in step 2) is mixed according to the wet weight ratio of the mi...

Embodiment 3

[0024] 1) Using 8 parts of Bacillus pantothenic acid, 15 parts of Brachybacterium wilterii, 25 parts of white rot fungi, 10 parts of Pseudomonas putida, 25 parts of Agrobacterium, 20 parts of Pseudomonas stutzeri, 25 parts of Enterobacter cloacae , inoculating the strains on the slant culture medium in the test tube, and culturing them at 25°C for 5 days;

[0025] 2) The above slant culture was made into a bacterial suspension with sterile water, and then 10 parts of Pseudomonas putida, 25 parts of Agrobacterium, 20 parts of Pseudomonas stutzeri, and 25 parts of Enterobacter cloacae were inoculated into the production organism. Cultivate separately in surfactant medium and culture at 25°C for 5 days; 8 parts of Bacillus pantothenica, 15 parts of Brevibacterium wilt, and 25 parts of white rot fungus were inoculated into bacterial culture medium and cultured at 25°C 5 days;

[0026] 3) The microbial fermentation liquid in step 2) is mixed according to the wet weight ratio of th...

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PUM

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Abstract

The invention relates to a compound bacterium colony for de-coloring papermaking industrial wastewater and a preparation method thereof. The compound bacterium colony is prepared from 2 to 10 parts of amycolata bacillus, 10 to 20 parts of curtobacterium flaccumfaciens, 20 to 30 parts of white rot fungi, 10 to 15 parts of pseudomonas putida, 20 to 30 parts of agrobacterium, 10 to 30 parts of pseudomonas stutzeri and 20 to 30 parts of enterobacter cloacae. According to the compound bacterium colon provided by the invention, microorganisms have a beneficial mutual effect and the microorganisms are mixed, cultured and domesticated; certain improvement is carried out on a flowing direction of intermediate metabolites of pollutants by bacteria and inhibitive intermediate metabolites are not generated or are transformed as soon as possible, so that the degradation efficiency of the pollutants is improved.

Description

technical field [0001] The invention relates to the field of papermaking wastewater treatment, in particular to a composite bacterial group for decolorizing papermaking industrial wastewater and a preparation method thereof. Background technique [0002] The water consumption situation of my country's paper industry is still high consumption, low efficiency, heavy pollution, which is extremely incompatible with my country's water resource conditions, and it is also one of the severe challenges that the paper industry is currently facing. After adopting technologies such as alkali recovery and biological treatment, one of the keys that affect papermaking wastewater to meet new discharge standards or reuse is the problem of chromaticity. [0003] Because the main component that causes the chromaticity of papermaking wastewater is lignin-based chromogenic substances, which have good chemical stability and are difficult to remove. The traditional methods mostly use strong oxidi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C02F3/34C12R1/07C12R1/645C12R1/40C12R1/01C12R1/38C02F103/28
CPCC12N1/20C02F3/34C02F3/347C02F2103/28C12N1/14
Inventor 李健菊陆文燕张坤黄鹏黄跃
Owner QINGDAO TECHNOLOGICAL UNIVERSITY
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