Compound bacterium colony for de-coloring papermaking industrial wastewater and preparation method thereof
A technology for paper industry wastewater and compound flora, which is applied in the process of wastewater treatment, microorganism-based methods, biochemical equipment and methods, etc. lower problem
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Embodiment 1
[0016] 1) Using 10 parts of Pantothenic acid bacillus, 10 parts of Bachybacterium wilterii, 20 parts of white rot fungi, 15 parts of Pseudomonas putida, 30 parts of Agrobacterium, 10 parts of Pseudomonas stutzeri, 30 parts of Enterobacter cloacae , inoculating the strains on the slant medium in the test tube, and culturing them at 30° C. for 3 days;
[0017] 2) The above slant culture was made into a bacterial suspension with sterile water, and then 15 parts of Pseudomonas putida, 30 parts of Agrobacterium, 10 parts of Pseudomonas stutzeri, and 30 parts of Enterobacter cloacae were inoculated into the production organism. Cultivate separately in surfactant medium, and cultivate at 30°C for 3 days; 10 parts of Bacillus panthenica, 10 parts of Brevibacterium wilt, and 20 parts of white rot fungi are inoculated into bacterial culture medium and cultured at 30°C 3 days;
[0018] 3) The microbial fermentation liquid in step 2) is mixed according to the wet weight ratio of the micr...
Embodiment 2
[0020] 1) Use 2 parts of Pantothenic acid bacillus, 10 parts of Brevibacterium wilterii, 30 parts of white rot fungi, 10 parts of Pseudomonas putida, 20 parts of Agrobacterium, 10 parts of Pseudomonas stutzeri, 20 parts of Enterobacter cloacae , inoculating the strains on the slant medium in the test tube, and culturing them at 25° C. for 7 days;
[0021] 2) The above slant culture was made into a bacterial suspension with sterile water, and then 10 parts of Pseudomonas putida, 20 parts of Agrobacterium, 10 parts of Pseudomonas stutzeri, and 20 parts of Enterobacter cloacae were inoculated into the production organism. Cultivate separately in surfactant medium, and cultivate at 25°C for 7 days; 2 parts of pantothenic acid bacillus, 10 parts of Brevibacterium wilt, and 30 parts of white rot fungi are inoculated into bacterial culture medium and cultured at 25°C 7 days;
[0022] 3) The microbial fermentation liquid in step 2) is mixed according to the wet weight ratio of the mi...
Embodiment 3
[0024] 1) Using 8 parts of Bacillus pantothenic acid, 15 parts of Brachybacterium wilterii, 25 parts of white rot fungi, 10 parts of Pseudomonas putida, 25 parts of Agrobacterium, 20 parts of Pseudomonas stutzeri, 25 parts of Enterobacter cloacae , inoculating the strains on the slant culture medium in the test tube, and culturing them at 25°C for 5 days;
[0025] 2) The above slant culture was made into a bacterial suspension with sterile water, and then 10 parts of Pseudomonas putida, 25 parts of Agrobacterium, 20 parts of Pseudomonas stutzeri, and 25 parts of Enterobacter cloacae were inoculated into the production organism. Cultivate separately in surfactant medium and culture at 25°C for 5 days; 8 parts of Bacillus pantothenica, 15 parts of Brevibacterium wilt, and 25 parts of white rot fungus were inoculated into bacterial culture medium and cultured at 25°C 5 days;
[0026] 3) The microbial fermentation liquid in step 2) is mixed according to the wet weight ratio of th...
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