Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method and application of optical biosensor for detecting steroid hormones in environment

A biosensor and detection environment technology, applied in the field of microbial detection, can solve the problems of high price and complicated operation, and achieve the effect of simple operation, fast, sensitive and accurate detection

Inactive Publication Date: 2017-12-22
CHANGCHUN UNIV OF SCI & TECH
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection of environmental steroid hormones is mainly based on large-scale imported instruments such as liquid phase and mass spectrometry, which are expensive and complicated to operate, and are only suitable for laboratory environmental detection, and can only detect a single component of known environmental steroids Therefore, it is urgent to establish a new method for rapid, sensitive, high-throughput, real-time and in situ detection of total environmental steroid hormones

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of optical biosensor for detecting steroid hormones in environment
  • Preparation method and application of optical biosensor for detecting steroid hormones in environment
  • Preparation method and application of optical biosensor for detecting steroid hormones in environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The present invention will be further described below in conjunction with accompanying drawing:

[0041] 1. Instrument: Biomolecular interaction detector based on solid-phase surface-enhanced light interference Reagent: Testosterone standard substance (Testosterone); 5'-Biotin modified single-stranded DNA fragment OP1; repressor protein RepA prokaryotic expression strain BL21-pET-15b -RepA; ultrapure water (resistance ≥ 18.2MΩ.cm);

[0042] 2. Using the repressor protein RepA prokaryotic expression strain BL21-pET-15b-RepA to express the RepA protein that can specifically bind to testosterone, the results are as follows image 3 shown. Synthetic single-stranded DNA (ssDNA) 10OD, its 5' end labeled with biotin (Biotin).

[0043] OP1: 5'bioin-GCAGCTCGGCCATGTCAAAGCCCAGCGGCCCCGCGCCC

[0044] 3. The preparation adopts the following steps: the bottom surface of the selected quartz fiber probe is used as the sensing element of the sensor, the SA protein is fixed to the lowe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses a preparation method and application of an optical biosensor for detecting steroid hormones in environment, the method is as follows: 1) using a covalent coupling technique to fix avidin as a sensing membrane of a sensor to the bottom end of quartz optical fiber; 2) putting the sensing membrane of the sensor into a sample hole containing no steroidal hormones and hormone binding proteins to observe the stability of a probe; 3) placing the sensor into a sample hole containing the steroid hormones and the hormone binding proteins; 4) placing the sensor into a diluted solution of a to-be-measured molecule; and 5) analyzing a combination dissociation curve for each concentration to obtain corresponding affinity constants. The application is as follows: the steroid hormone content in the environment is determined. The beneficial effects are as follows: high specificity, high sensitivity, biosafety, and easy operation.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection. Background technique [0002] Steroid hormones are cyclopentane polyhydrophenanthrene derivatives containing cyclopentane polyhydrophenanthrene skeleton structure, including sex hormones and adrenal cortex hormones, which are extremely important in maintaining life, regulating sexual function, treating immune diseases, and birth control. role. Studies have shown that undecomposed steroid hormones, such as sex hormones and synthetic hormone drugs, are discharged into the environment in large quantities in the excreta of humans and livestock and poultry. Synthetic hormone substances can mimic physiological hormones to induce responses to organisms , interfere with metabolic signaling pathways in the body, and belong to a class of carcinogens. [0003] At present, the detection of environmental steroid hormones is mainly based on large imported instruments such as liquid phase and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/76
CPCG01N21/763
Inventor 郭瑞潘琳于源华张昊宫平张晓
Owner CHANGCHUN UNIV OF SCI & TECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com