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High-content avocado kernel procyanidine, preparation method and application of high-content avocado kernel procyanidine

A technology of proanthocyanidins and avocado fruit, which is applied in the fields of anti-toxic agents, metabolic diseases, organic chemistry, etc., can solve the problems such as the extraction and purification process of proanthocyanidins that have not been mentioned, and achieve the effect of simple preparation method, excellent hypoglycemic effect, and good scavenging ability

Active Publication Date: 2017-12-29
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, pure water or 10-50% ethanol aqueous solution is used as a solvent to extract avocado powder, and the extract is purified step by step by D3520 resin to obtain polyphenol extract, but this method does not mention the extraction of proanthocyanidins Purification process

Method used

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  • High-content avocado kernel procyanidine, preparation method and application of high-content avocado kernel procyanidine
  • High-content avocado kernel procyanidine, preparation method and application of high-content avocado kernel procyanidine
  • High-content avocado kernel procyanidine, preparation method and application of high-content avocado kernel procyanidine

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Embodiment 1

[0034] The preparation method of the high-content avocado stone proanthocyanidins of the present embodiment comprises the steps:

[0035] Step 1: Avocado stones are pulverized, dried, and passed through a 20-mesh sieve to obtain avocado stone powder, add water, and perform ultrasonic extraction twice, each time for 60 minutes, with a solid-to-liquid mass ratio of 1:10, combine the extracts, concentrate, After spray drying to a water content of 10%, the crude extract of avocado stones is obtained;

[0036] Step 2: Using a mixture of ethyl acetate, n-butanol and water at a volume ratio of 40:(5-20):50 as a two-phase solvent system, extract the crude avocado core extract obtained in step 1, and collect the upper layer The extract is spray-dried to a water content of 10% to obtain an extract rich in proanthocyanidins;

[0037] Step 3: The proanthocyanidin-rich extract obtained in step 2 is purified by high-speed countercurrent chromatography, and the mixture of ethyl acetate, n-b...

Embodiment 2

[0042] A kind of preparation method of high-content avocado fruit stone proanthocyanidins of the present embodiment comprises the following steps:

[0043] Step 1: the avocado stone is pulverized, dried, and passed through a 30-mesh sieve to obtain avocado stone powder, adding 60% ethanol by mass percentage, ultrasonically extracting 3 times, each time for 30 minutes, the mass ratio of solid to liquid is 1:20, The extracts were combined, concentrated, and vacuum-dried to a water content of 12%, to obtain the crude extract of avocado core;

[0044] Step 2: The crude extract of the avocado core obtained in step 1 is adsorbed by the macroporous resin HPD826, and the impurities are removed with a 5% ethanol aqueous solution, and the ethanol eluate with a mass percentage of 55% is collected, and vacuum-dried to a moisture content After being 12%, the extract rich in proanthocyanidins is obtained;

[0045] Step 3: The proanthocyanidin-rich extract obtained in step 2 is purified by ...

Embodiment 3

[0050]A kind of preparation method of high-content avocado fruit stone proanthocyanidins of the present embodiment comprises the following steps:

[0051] Step 1: Avocado stones are pulverized, dried, and passed through a 40-mesh sieve to obtain avocado stone powder, add 60% by mass methanol aqueous solution, ultrasonically extract 3 times, each time for 40 minutes, and the mass ratio of solid to liquid is 1:25 , combining the extracts, concentrating, freeze-drying to a water content of 15%, and obtaining the crude extract of avocado core;

[0052] Step 2: The avocado core crude extract obtained in step 1 is separated by a C18 column, and impurities are removed with 10% ethanol aqueous solution, and 30% ethanol eluate is collected, and freeze-dried until the water content is After 15%, an extract rich in proanthocyanidins is obtained;

[0053] Step 3: The proanthocyanidin-rich extract obtained in step 2 is purified by high-speed countercurrent chromatography, and a mixture of...

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Abstract

The invention discloses high-content avocado kernel procyanidine, a preparation method and application of the high-content avocado kernel procyanidine, and belongs to the technical field of comprehensive utilization and development of avocado resources. The preparation method comprises the following steps: taking avocado kernel crude extracts; then gathering the avocado kernel crude extracts to obtain an extract which is rich in procyanidine; and finally carrying out high-speed countercurrent chromatography purification on the extract, separating the extract by a high-speed countercurrent separation system, collecting effluent, condensing and drying to obtain the avocado kernel procyanidine. The invention further discloses the high-content avocado kernel procyanidine prepared by the method and application of the high-content avocado kernel procyanidine. The content of the avocado kernel procyanidine is counted by the equivalent weight of procyanidine B2, and is not lower than 90%, the hypoglycemic effect of the avocado kernel procyanidine is higher than that of acarbose, the free radical scavenging capacity of the avocado kernel procyanidine is equal to or better than that of vitamin C, and the high-content avocado kernel procyanidine has various bioactivity effects of high-activity blood glucose reduction, free radical scavenging, oxidation resistance and the like.

Description

technical field [0001] The invention relates to a high-content avocado stone proanthocyanidin, a preparation method and an application thereof, and belongs to the technical field of comprehensive utilization and development of avocado resources. Background technique [0002] Avocado (Persea Americana Mill), also known as avocado, avocado, happiness fruit, etc., is an emerging tropical and subtropical famous fruit, rich in essential fatty acids, proteins, minerals and various vitamins, in Central America (originally Origin) enjoys the reputation of "the source of life". In addition to the rich fatty acids in the pulp, other parts of the avocado, such as the peel and stone, also contain rich antioxidant components, all of which have high application and development value prospects. [0003] At present, the analysis of the active components of avocado stone and the research on anti-oxidation have been reported, but the purification process is rarely involved. Chinese Patent A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62A61P3/10A61P39/06
CPCC07D311/62
Inventor 宁德生潘争红符毓夏李连春
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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