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Preparation method of medical and edible fungus mycelium polysaccharide-polypeptide immune enhancer

An immune enhancer, polysaccharide polypeptide technology, which is applied in the field of preparation of medicinal and edible fungi mycelial polysaccharide polypeptide immune enhancer, can solve the problems of poor immune effect, singleness, low immune response, etc., and achieves high efficiency, simple process, and improved resources. utilization rate and economic benefits

Active Publication Date: 2018-01-05
肖兵南
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0022] Most of the functions of polysaccharides and polypeptides are similar, especially in immune regulation. Most of the applied research is a single type of applied research; it is of great significance to study the compound preparation of polysaccharides and peptides and to measure the effect
[0023] At present, the immunomodulators of polypeptides are mostly oral preparations. The research and development of injections is conducive to improving bioavailability, convenient administration, and can be used together with vaccines to solve the problems of low immune response and poor immune effect in animals.

Method used

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  • Preparation method of medical and edible fungus mycelium polysaccharide-polypeptide immune enhancer
  • Preparation method of medical and edible fungus mycelium polysaccharide-polypeptide immune enhancer
  • Preparation method of medical and edible fungus mycelium polysaccharide-polypeptide immune enhancer

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Embodiment Construction

[0053] 1. Preparation method of edible and medicinal fungal mycelia polysaccharide polypeptide:

[0054] (1) Preparation of mycelial fermentation product: culture pine milk mushroom, enoki mushroom, shiitake mushroom, Hericium erinaceus, matsutake and other edible and medicinal fungal mycelium by liquid submerged fermentation method, and prepare edible and medicinal fungal mycelium fermentation product, including mycelium and fermentation liquid. The fermentation conditions are shown in Table 1.1:

[0055] Table 1.1 Preparation of edible and medicinal fungal mycelia liquid fermentation product

[0056]

[0057] (2) Treatment of mycelia fermentation: take the liquid mycelia fermentation product (mycelia and fermentation broth) and process through grinding and high-pressure homogenization to break the mycelium and release the polysaccharide polypeptide; add 0.05-0.2% (w / v ) compound enzyme (cellulase: pectinase = 1.5:2-3) for the first enzymatic hydrolysis (PH5, 50°C, 2-3h)...

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Abstract

The invention discloses a preparation method of a medical and edible fungus mycelium polysaccharide-polypeptide immune enhancer. The preparation method comprises efficient extraction of mycelium polysaccharide polypeptide based on combination of a physical method and enzymatic hydrolysis, ethanol-based precipitation separation, precipitation purification respectively with levamisole and high-concentration ethanol, and blending preparation of compound immunity-enhancing injection preparations. Through mixing with a vaccine or compounding with an injection, animal immunity is improved, production efficiency is high and a relative cost of production is low. The lactarius deliciosus mycelium polysaccharide is simpler than lactarius deliciosus mycelium monosaccharide and can be further decomposed into rare functional monosaccharide. The preparation method has simple processes.

Description

technical field [0001] The invention relates to a preparation method of a medicinal and edible fungus mycelia polysaccharide polypeptide immunopotentiator. Background technique [0002] Functional substances of edible and medicinal fungi: In addition to nutrients such as proteins, amino acids, fats, vitamins and trace elements, edible and medicinal fungi also contain a large amount of active substances such as polysaccharides, polypeptides, nucleosides, and terpenes. It has strong anti-tumor, anti-oxidation, immune regulation, anti-virus, anti-bacterial and anti-parasitic functions, as well as the effect of treating diabetes, cardiovascular disease and hypercholesterolemia, and has the functions of preventing, curing and regulating human physiology Functional efficacy. In recent years, scholars at home and abroad have been very active in the research on the active substances in edible fungi, especially on the biologically active polysaccharides and active protein polypeptid...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12P21/00C07K1/14C07K1/30C07K1/34C08B37/00A61K36/07A61K39/39A61P37/04
Inventor 刘莹莹肖毅肖兵南蒋小文
Owner 肖兵南
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