Portable fully-integrated DNA on-site inspection minitype total-analysis system detection light path

Abstract

The invention relates to a portable fully-integrated DNA on-site inspection minitype total-analysis system detection light path. The portable fully-integrated DNA on-site inspection minitype total-analysis system detection light path is characterized by comprising an exciting light focusing system, a micro-fluidic electrophoresis chip, a fluorescence collecting, focusing and conducting system as well as a fluorescence splitting and detection system. The portable fully-integrated DNA on-site inspection minitype total-analysis system detection light path can divide wide-wavelength fluorescence into a plurality of colored tapes; and besides, a focusing mirror in a wavelength division multiplexer can focus fluorescence beams into micron-order light spots, and is suitable for a low-noise detector and a detector with high noise caused by large sensitive surface. The portable fully-integrated DNA on-site inspection minitype total-analysis system detection light path has a light path structurewhich is provided with a plurality of fluorescence channels, is high in flux and high in flexibility and is simple in process adjustment and low in cost, realizes modular independence, is simple in process adjustment, high in stability and high in extensible degree of electrophoresis channels and fluorescence channels, and can be widely applied in the fields of DNA detection, on-site detection, capillary electrophoresis detection and the like.

Description

technical field [0001] The invention relates to a detection optical path of a miniature total analysis system for portable fully integrated DNA on-site inspection, belonging to the technical field of biological detection. Background technique [0002] DNA sequencing technology is a biophysical technology used in the engineering of nucleic acids, proteins, cells, etc. In DNA sequencing technology, capillary electrophoresis separation technology makes DNA fragments of different lengths move in the pipeline at different speeds, and fluorescence detection technology performs multi-parameter analysis of electrophoretic patterns on DNA fragments of different lengths marked with different fluorescent information. Microchip technology is based on analytical chemistry and based on micro-electromechanical system processing technology. It combines micro-analysis chip system technology with integrated technology, and integrates sampling, dilution, sample injection, separation, and detec...

AI Technical Summary

Problems solved by technology

The optical path of the existing capillary electrophoresis detection platform mostly adopts the galvanometer scanning method, and the fluorescence enters the detector CCD or CMOS after being split by the grating. For example, the patent CN104641217A discloses a flow cytometer, which discloses a large numerical aperture and wide output collimation. The combination of beam fluorescence microscope and WDM detects the fluorescence of cells labeled with fluorescence. WDM is used to segment multi-wavelength fluorescence, but the relay imaging mirror with its built-in WDM structure uses a concave mirror. The purpose is to make the collimated beam path Effectively double the beam diameter without enlarging the beam diameter. However, this will definitely use a doublet lens at the incident fluorescence to eliminate the chromatic aberration generated at the concave mirror. This design structure has complex optical structure of the system, difficult debugging, and requirements for machining accuracy. High, high mechanical cost, high optical cost

[0005] In summary, the microfluidic capillary chip in the fully integrated DNA on-site inspection system has certain limitations, and the integration of the microfluidic chip and the detection system cannot be realized, and the detection of the portable fully integrated DNA microanalysis system cannot be achieved The main problems are: 1) When used in microfluidic chip capillary electrophoresis, the light source adopts a point light source. In the case of high radial resolution, the laser power requirements are high and the cost is high; 2) The galvanometer scanning method, The working distance is required to be short and the focal length of the objective lens is small, so the scanning stroke will be short, the flux of the electrophoresis channel will be small, the efficiency will be reduced, and the debugging process will be difficult. If you want to improve the efficiency, you need to increase the focal length of the scanning objective lens, and the numerical aperture will decrease. Fluorescence sensitivity decreases, and the fluorescence acquisition performance of the instrument decreases, which requires an increase in the sample volume and an increase in the efficiency of the extraction, amplification, and electrophoresis steps, thereby increasing the difficulty of the entire process; Large size, high cost, and reduced stability of the whole machine; 4) The use of grating light splitting requires good calibration of the fluorescence entering the grating, high performance requirements for the objective lens, complex design, difficult process, and high cost. The position is unique relative to the objective lens, requiring a large space, and the space arrangement is inflexible; the matching detector, in order to improve the detection sensitivity, CCD or CMOS is equipped with a cooling module, which is large in size and high in cost; 5) Spatial spectroscopic mode takes up space large and unique

In view of the shortcomings and limitations of the above existing technologies, the integration of the microfluidic chip and the detection system cannot be realized, and the detection requirements of the portable fully integrated DNA micro-analysis system cannot be met.

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