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Method for separating cynomorium songaricum polysaccharides through deep-eutectic solvent/salt two-aqueous-phase extraction

A technology of deep eutectic solvent and Cynomorium polysaccharide, which is applied in the field of deep eutectic solvent/salt two-phase extraction and separation of Cynomorium polysaccharide, which can solve the problems of low extraction rate of Cynomorium polysaccharide, cumbersome operation steps, and large consumption of reagents, etc. problems, achieve the effects of mild conditions, wide sources of raw materials, and reduced production costs

Active Publication Date: 2018-01-19
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a kind of deep eutectic solvent / salt two-phase extraction method for separating Cynomorium polysaccharides, to solve the problem of low extraction yield of Cynomorium polysaccharides in the prior art, large consumption of reagents, high energy consumption and high price. Expensive, cumbersome operation steps and other issues

Method used

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  • Method for separating cynomorium songaricum polysaccharides through deep-eutectic solvent/salt two-aqueous-phase extraction

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Embodiment 1

[0027] A method for separating Cynomorium polysaccharide by deep eutectic solvent / salt two-phase extraction, the Cynomorium raw material used in the method is the Cynomorium powder produced in Gansu after degreasing and natural drying, and the method comprises the following steps:

[0028] Step 1. Preparation of Cynomorium polysaccharide crude extract: take 1 g of Cynomorium powder, add 20 mL of 40% choline chloride-malic acid deep eutectic solvent with a volume percentage of water content, mix well, and place in an ice bath at 4°C Ultrasonic treatment is carried out under the conditions, the ultrasonic treatment power is 300W, the pulse working mode is ultrasonic 2s, intermittent 2s, ultrasonic time is 30 minutes, then the solution is centrifuged at 4000 rpm for 5 minutes, and the supernatant is taken to obtain Cynomorium Polysaccharide crude extract;

[0029] Step 2, two-phase extraction: add 10 g of dipotassium hydrogen phosphate powder to the crude extract of Cynomorium po...

Embodiment 2

[0034] A method for separating Cynomorium polysaccharide by deep eutectic solvent / salt two-phase extraction, the Cynomorium raw material used in the method is the Cynomorium powder produced in Gansu after degreasing and natural drying, and the method comprises the following steps:

[0035] Step 1. Preparation of Cynomorium polysaccharide crude extract: take 1g Cynomorium powder, add 30mL of 50% choline chloride-glycerol deep eutectic solvent with a volume percentage of water content, mix well, under 4 ℃ ice bath condition Carry out ultrasonic treatment, the ultrasonic treatment power is 350W, the pulse working mode is ultrasonic for 2s, the interval is 2s, and the ultrasonic time is 40 minutes, then the solution is centrifuged at 3000 revolutions per minute for 10 minutes, and the supernatant is taken to obtain Cynomorium polysaccharide crude. extract;

[0036] Step 2, two-phase extraction: add 15g of solid dipotassium hydrogen phosphate powder to the crude extract of Cynomori...

Embodiment 3

[0041] A method for separating Cynomorium polysaccharide by deep eutectic solvent / salt two-phase extraction, the Cynomorium raw material used in the method is the Cynomorium powder produced in Gansu after degreasing and natural drying, and the method comprises the following steps:

[0042]Step 1. Preparation of Cynomorium polysaccharide crude extract: Take 1g Cynomorium powder, add 30mL of 45% choline chloride-succinic acid deep eutectic solvent with a volume percentage of water content, mix well, and place in an ice bath at 4°C. Ultrasonic treatment was carried out under the following conditions, the ultrasonic treatment power was 400W, the pulse working mode was ultrasonic 2s, the interval was 2s, and the ultrasonic time was 50 minutes, and then the solution was centrifuged at a speed of 3000 revolutions per minute for 10 minutes, and the supernatant was taken to obtain Cynomorium polysaccharide. crude extract;

[0043] Step 2, two-phase extraction: add 10g of solid dipotass...

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Abstract

The invention discloses a method for separating cynomorium songaricum polysaccharides through deep-eutectic solvent / salt two-aqueous-phase extraction. The method comprises the following main steps: adding a deep-eutectic solvent with a certain moisture content into cynomorium songaricum powder, fully leaching at room temperature, performing ultrasonic pulse treatment under ice-bath conditions, andcentrifuging to collect the supernatant so as to obtain a crude cynomorium songaricum polysaccharide extract; adding dipotassium phosphate into the crude cynomorium songaricum polysaccharide extract,mixing, dissolving, standing and centrifuging to form a two-aqueous-phase extraction system, wherein the upper phase is the deep-eutectic solvent, and the lower phase is a solution containing sugar and dipotassium phosphate; performing freeze drying recovery on the upper phase; and dialyzing the lower phase, and performing freeze drying, thereby obtaining the cynomorium songaricum polysaccharide.The deep-eutectic solvent adopted in the invention has the advantages of being non-toxic, excellent in biodegradability and biocompatibility and low in melting point, the extraction, separation and purification operations are simple, the conditions are mild, the separation efficiency is high, the yield of the cynomorium songaricum polysaccharide is high, the deep-eutectic solvent can be recycled,the production cost is effectively reduced, and the method is suitable for large-scale industrial production.

Description

technical field [0001] The field relates to the technical field of deep processing of natural products, in particular to a method for separating Cynomorium polysaccharide by deep eutectic solvent / salt two-phase extraction and separation. Background technique [0002] Cynomorium (scientific name: Cynomorium songaricum Rupr.), Cynomoraceae, Cynomorium is a perennial succulent parasitic herb, without chlorophyll, the whole plant is reddish-brown, mostly buried in sand, mainly distributed in northwest China, as one of the commonly used medicinal materials in Chinese and Mongolian medicine, with improved Various effects such as immunity, disease prevention and anti-cancer; Cynomorium contains a variety of chemical and medicinal components; Cynomorium polysaccharide, as one of the most important active ingredients, is a natural macromolecular compound formed by the dehydration and condensation of multiple monosaccharide molecules , is another large class of molecules that transmi...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 张继张喜峰滕桂香丁玲
Owner NORTHWEST NORMAL UNIVERSITY
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