Preparation method of compound microorganism bacterium agent for aquaculture
A compound microbial agent and aquaculture technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of bottom pollution and secondary pollution of reclaimed water, achieve easy volatilization, increase yield and quality , good stability and high efficiency
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[0030] A kind of preparation method of composite microbial bacterial agent for aquaculture of the present invention comprises the following steps:
[0031] (1) Add the mixed bacteria powder to the primary culture solution for activation and mixed culture. The mass ratio of the mixed bacteria powder to the primary culture solution is 1:9 to obtain the mixed bacteria solution, and then add the mixed bacteria solution to the secondary culture solution , the amount of the mixed bacterial solution is 40% of the volume of the secondary culture solution. Under the condition of 30~35℃, expand the culture to the logarithmic growth phase and adjust the OD 600 value, if OD 600 >1.5, dilute to OD with secondary culture medium 600 =1.5, if OD 600 600 =1.5, to be dried mixed bacteria solution;
[0032] (2) Put the above-mentioned mixed bacterial solution to be dried into a centrifuge, centrifuge at 5000 r / min for 15 minutes, remove the supernatant to obtain a precipitate, and mix the pre...
example 1
[0036] Add the mixed bacteria powder to the primary culture solution for activation and mixed culture for 10 hours. The mass ratio of the mixed bacteria powder to the primary culture solution is 1:9 to obtain a mixed bacteria solution, then add the mixed bacteria solution to the secondary culture solution, and mix The amount of bacterial solution added is 40% of the volume of the secondary culture solution. Under the condition of 30°C, expand the culture to the logarithmic growth phase and adjust the OD 600 value, if OD 600 >1.5, dilute to OD with secondary culture medium 600 =1.5, if OD 600 600 =1.5, the mixed bacterial solution must be dried.
[0037] The agar disc diffusion method was used to detect whether there was any inhibitory effect among the three bacteria.
[0038] The specific growth conditions of the mixed bacterial solution are shown in Table 1
[0039] Table 1
[0040] bacteria
[0041] The experimental results showed that there was no antagonisti...
Embodiment 2
[0043] Preparation of mixed bacterial powder: Bacillus subtilis, Candida utilis and Bifidobacterium were mixed evenly at a mass ratio of 2:1:1.
[0044] Preparation of the primary culture medium: the formula of the primary culture medium is calculated in parts by weight. Take 1.2 parts of beef extract, 2.5 parts of tryptone, 8 parts of whole potato powder, 12 parts of agar, 1.5 parts of glucose, 3 parts of NaCl, KH 2 PO 4 1.2 parts, Tween-80 0.6 parts, cysteine 0.2 parts, water 1000 parts, pH value 7.0 ± 0.2, sterilized at 121 ℃ for 30 minutes to obtain the primary culture medium.
[0045] Preparation of the secondary culture liquid: the formula of the secondary culture liquid is calculated in parts by weight. Take 12 parts of tryptone, 1 part of peptone, 0.2 part of beef extract, 20 parts of maltose, 10 parts of glucose, 1 part of NaCl, and 15 parts of agar. parts, KH 2 PO 4 1.2 parts, MgSO 4 ·7H 2 0.8 parts of O, 5 parts of vitamins, 1000 parts of water, pH value 7....
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