Au@AgNCs-based sulfur ion detection method

A sulfur ion and sulfur measurement technology, which is applied in Raman scattering, material excitation analysis, etc., can solve the problem that the detection method is difficult to meet the needs of research, and achieve the effect of high sensitivity

Inactive Publication Date: 2018-01-19
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, these detection methods are difficult to meet the needs of research

Method used

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  • Au@AgNCs-based sulfur ion detection method
  • Au@AgNCs-based sulfur ion detection method
  • Au@AgNCs-based sulfur ion detection method

Examples

Experimental program
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Embodiment 1

[0029] Step 1. Preparation of gold nanosphere solution:

[0030] a. 0.01mol / L NaBH in an ice-water bath 4 The solution was added to 0.01mol / L CTAC solution and 0.01mol / L HAuCl 4 In the mixed solution of the solution, the solution changed from yellow to brown in an instant. After stirring for 3 minutes, the solution was placed in a 28°C water bath for 2 hours;

[0031] b. Add 0.2mol / L CTAC solution, 0.0005mol / L HAuCl to the reaction flask 4 Solution, 0.1mol / L AA solution, after mixing evenly, add the seed solution of step a diluted 5 times. After stirring for 5 minutes, put it in a water bath at 36°C for 1 hour;

[0032] c. Add 0.2mol / L CTAC solution, 0.0005mol / L HAuCl to the reaction flask 4 Solution, 0.1mol / L AA solution, mix well and add the seed solution of step b. Put it in a water bath at 36° C. for 1 hour to obtain a gold nanosphere solution (spherical gold nanoparticle solution).

[0033] Step 2. Preparation of Au@AgNCs particles:

[0034] Add 0.2 mol / L CTAC solu...

Embodiment 2

[0036] Step 1. The gold-core-silver-shell nanocubes (Au@AgNCs) with a size of 40-100 nm prepared in Example 1 were prepared into a solution for later use, and the solution also contained 1 μmol-10 mmol of CTAB or CTAC as a stabilizer.

[0037] Step 2. Preparation of the plasma scattering test chip: add ultrapure water to a clean centrifuge tube, and then add an appropriate amount of the Au@AgNCs solution obtained in step 1 to dilute 5-50 times. The glass (thickness: 0.1-2mm) cleaned with ether, ethanol, and water in turn is immersed in the above diluted solution, and the AgNCs are fixed on the surface of the glass sheet by electrostatic adsorption. After soaking for 5-60s, take it out, rinse with ultrapure water to remove unadsorbed particles, and finally blow dry the glass sheet with nitrogen. The prepared glass sheet immobilized with Au@AgNCs is the plasma scattering test chip, and the nanoparticles attached to the glass sheet are the nanoprobes.

[0038] Step 3. Place the ...

Embodiment 3

[0042] Place the test chip prepared in Example 2 on the stage of a dark-field microscope, add 180 μL of ultrapure water to the center of the surface of the glass slide to form a circular water droplet, and move the operating platform so that the water droplet is located in the light path the very center of . Color images of the particles were collected under a dark-field microscope, followed by scattering spectra of individual nanoparticles under a microscattering spectrometer. Then add 20 μL of 10 -4 M sulfide ion solution, timing and real-time monitoring of the spectral changes of the scattering peaks of the particles caused by the reaction between the probe and sulfide ions. By repeating the above experimental operation, a single probe can detect 10 -5 ~10 -9 M Sulfide, such as figure 2 shown. It can be found that the probe can quickly detect sulfur ions within 2 minutes, and basically detect the concentration level of sulfur ions within 10 minutes.

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Abstract

The invention discloses an Au@AgNCs-based sulfur ion detection method, which comprises: preparing an Au@AgNCs immobilized glass sheet as a plasma scattering test chip; placing the plasma scattering test chip on the object stage of a dark field microscope; adding 50-500 [mu]L of ultrapure water at the center position of the glass sheet surface to form a round water bead; making the water bead be positioned at the center of a light path by moving an operation platform; collecting the color pictures of the particles under the dark field microscope; collecting the scattering spectrums of the single nanoparticle under a micro-area scattering spectrometer; adding a sulfur ion solution to be detected to the water bead, and observing the scattering spectrum peak shift variation of the single nanometer probe; and finally calculating the standard curve between the sulfur ion concentration and the scattering spectrum movement, and calculating the sulfur ion concentration in the solution to be detected by comparing to the standard curve. According to the present invention, the detection method is based on the single particle LSPR technology, and has advantages of real-time property, fastness,accuracy, high sensitivity, and the like.

Description

technical field [0001] The invention belongs to the field of biological application of nanomaterials, and in particular relates to a method for detecting sulfur ions based on Au@AgNCs. Background technique [0002] Due to the unique LSPR optical properties of gold-silver core-shell nanocrystals, they can be widely used in optics, catalysis, information storage, chemical biosensing, and surface-enhanced Raman. In recent years, it has attracted more and more researchers' attention. In order to study their specific properties for better broad application, the researchers used different chemical methods to prepare gold-silver nanocrystals in different shapes, including spheres, cubes, decahedrons, dodecahedrons, flakes and wires. At the same time, people are also trying to combine gold and silver into a single system, which provides a new method for regulating optical properties and catalysis. In conclusion, alloys and core-shell structures of gold and silver are widely used i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 张磊张军霞张颖汪联辉范曲立沈晶晶黄维
Owner NANJING UNIV OF POSTS & TELECOMM
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