Radix stemonae polysaccharide, blended and modified chitosan membrane and application in preparation of anti-adhesion membrane material

Abstract

The invention discloses radix stemonae polysaccharide, a blended and modified chitosan membrane and an application in preparation of an anti-adhesion membrane material. The radix-stemonae polysaccharide is obtained by adopting radix stemonae as a material, obtaining crude polysaccharides by water extraction and alcohol precipitation and protein removal, and dialyzing the crude polysaccharides by using a dialysis bag to intercept polysaccharides with the molecular weights of 15000-25000kD. The chitosan membrane blended and modified by the polysaccharides is prepared by adopting the chitosan andthe radix stemonae polysaccharide as materials and carrying out coating, drying, alkaline washing and drying again. The molecular weight of the radix stemonae polysaccharide provided by the inventionis 15000-25000kD, the modified chitosan membrane prepared by blending and modifying the radix stemonae polysaccharide with the molecular weight and the chitosan not only has good biocompatibility, but also has suitable degradation speed in the body, can be completely degraded in about 4 weeks, can inhibit postoperative adhesion effectively, has an anti-inflammatory effect, and can be used as an anti-adhesion material for operation.

Description

technical field [0001] The invention belongs to the field of materials and relates to an anti-adhesion film material, in particular to a polysaccharide blended with modified chitosan and an application in preparing an anti-adhesion film material. Background technique [0002] Postoperative adhesion is one of the key issues affecting the effect of surgery and even the success of surgery. Adhesions are abnormal structures in which fibrous bands of connective tissue join adjacent tissues or organs and can range in extent from a thin film to dense vascular scar tissue. Various studies have shown that the formation of adhesions is part of the normal tissue healing process, and the use of various anti-adhesion measures is to achieve a balance between tissue healing and adhesion formation, while reducing adhesion formation, it will not interfere with normal tissue healing. tissue healing. The mastery of this balance point is the difficult problem of preventing adhesion formation....

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Problems solved by technology

Although the introduction of gelatin improves the degradation rate of chitosan in vivo, the anti-blocking effect of this chitosan / gelatin blend film is not ideal

For example, Zhou Xilai et al. found that the adhesion degree of chitosan / gelatin blend film was not only stronger than that of the control group, but also stronger than that of the pure chitosan film treatment group. They believed that the reason for this phenomenon might be that although the introduction of gelatin accelerated The degradation rate of the blended membrane was lowered, but the blended membrane was not completely degraded 2 weeks after the operation, and the residual blended membrane could still cause the body’s foreign body reaction, and gelatin, as a polypeptide mixture, may have antigenicity, causing the body Generate immune rejection, thereby promoting the formation of local adhesions (Reference: Chitosan / gelatin blend film on the formation of peritoneal adhesions in rats, 2013)

Shahram et al. prepared a series of chitosan / gelatin composite films by changing the proportion of components, and explored its adhesion prevention effect in the rat cecum model. The study found that: when the content of chitosan in the blend film was 25% When the content of chitosan exceeds 25%, the anti-adhesion effect of the blend film is not significant, and when the content of chitosan exceeds 25%, it will not only fail to reduce the formation of adhesion, but also cause inflammation (reference: Evaluation of chitosan-gelatin films for use as postoperative adhesion barrier in ratcecum model, 2013)

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