Method for preparing clenbuterol hydrochloride molecularly imprinted membrane electrochemical light emitting sensor

A technology of clenbuterol hydrochloride and molecularly imprinted membranes, which is applied in the fields of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis through chemical reactions of materials, etc., to achieve a wide linear range, high sensitivity, and long service life. Effect

Inactive Publication Date: 2018-02-09
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] So far, there has been no report on the combination of electrochemiluminescence and molecularly imprinted polymers for the detection of clenbuterol hydrochloride

Method used

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  • Method for preparing clenbuterol hydrochloride molecularly imprinted membrane electrochemical light emitting sensor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Electrode pretreatment

[0046] Before preparation, the surface of the glassy carbon electrode was polished with 1.0, 0.3, and 0.05 μm alumina powder in sequence, rinsed with double distilled water, and then mixed with 0.2mol / L KNO 3 1mmol / L K 3 [Fe(CN) 6 ] The solution was scanned in the range of 0.6 to -0.2V by cyclic voltammetry until a stable electrochemical response was obtained, and then dried in the air;

[0047] (2) Electrodeposition of graphene oxide

[0048] First the 2mg / mL graphene oxide dispersion was diluted to 0.5mg / mL with double distilled water, and the glassy carbon electrode treated in step (1) was immersed in 3mL graphene oxide dispersion (0.5mg / mL). Electrodeposit 5 circles in the range of 0.6~-1.4V, and the scanning speed is 50mV / s, and the electrode modified by reduced graphene oxide is obtained. The electrodeposition solution was blown with nitrogen for 5 min to remove dissolved oxygen before use.

[0049] (3) Drop-coating up-converting ...

Embodiment 2

[0060] (1) Electrode pretreatment

[0061] Before preparation, the surface of the glassy carbon electrode was polished with 1.0, 0.3, and 0.05 μm alumina powder in sequence, rinsed with double distilled water, and then mixed with 0.2mol / L KNO 3 1mmol / L K 3 [Fe(CN) 6 ] The solution was scanned in the range of 0.6 to -0.2V by cyclic voltammetry until a stable electrochemical response was obtained, and then dried in the air;

[0062] (2) Electrodeposition of graphene oxide

[0063] Dilute the carboxylated graphene oxide dispersion of 2 mg / mL to 0.5 mg / mL with double distilled water, and immerse the glassy carbon electrode treated in step (1) in 3 mL of graphene oxide dispersion (0.5 mg / mL) Among them, 5 circles of electrodeposition were performed in the range of 0.6-1.4V, and the scanning speed was 50mV / s to obtain a reduced graphene oxide-modified electrode. The electrodeposition solution was blown with nitrogen for 5 min to remove dissolved oxygen before use.

[0064] (3) ...

Embodiment 3

[0075] Determination of clenbuterol hydrochloride content in actual samples:

[0076] Using the molecularly imprinted electrochemiluminescence sensor of Example 2 of the present invention, the clenbuterol hydrochloride in the actual samples (pork, pig liver, pig kidney) was analyzed and measured, no clenbuterol hydrochloride was detected, and the standard addition method was adopted Perform spike recovery experiments. The electrochemiluminescence and cyclic voltammetry simultaneous detection mode of the LK5100 electrochemiluminescence analysis system was selected for scanning measurement of the sample solution to be tested, the scanning voltage was 0-2.5V, and the photomultiplier tube voltage was 800V. Read the luminescence intensity after the electrochemiluminescence quenching, and substitute into (F 0 -F) / F 0 =0.1367lgC CLB +1.1311, calculates C CLB . Three parallel measurements were performed, and the calculated recovery rate was 89.3-100.4%, indicating that the molecu...

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Abstract

The invention provides a method for preparing a clenbuterol hydrochloride molecularly imprinted membrane electrochemical light emitting sensor. The method is characterized in that the surface of a glassy carbon electrode is wrapped by a composite film, and the composite film consists of reduced oxidized graphene, upconversion nanoparticles and molecular imprinting polymers. The method has the advantages that electrochemical light emission of the upconversion nanoparticles with stable cathode signals and high light emission strength are combined with the molecular imprinting polymers with goodselectivity, and trace detection on clenbuterol hydrochloride in animal derived foods can be implemented.

Description

technical field [0001] The invention belongs to the technical field of preparation of photoelectrochemical sensing materials, and in particular relates to a preparation method of a clenbuterol hydrochloride molecularly imprinted film electrochemical luminescence sensor based on upconversion nanoparticles. Background technique [0002] Due to the advantages of large Stokes shift, stable fluorescence signal, low toxicity, and good biocompatibility, upconversion nanoparticles are widely used in the field of optical sensors. But there are few reports in the field of electrochemiluminescence. As a new type of electrochemiluminescent material, upconversion nanomaterials have stable cathode signal and high electrochemiluminescence intensity, and have broad application prospects in the field of electrochemiluminescence. Molecularly imprinted polymers have high selectivity and affinity, are simple to prepare, low in cost, and can specifically recognize analyte substances. They can b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/48
Inventor 方国臻晋新翠刘冰王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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