Composite membrane material for releasing nitric oxide by magnetic control as well as preparation method and application of composite membrane material

A technology of nitric oxide and composite membrane, applied in the field of biomedical engineering materials

Inactive Publication Date: 2018-02-27
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, by ferric oxide (Fe 3 o 4 ) surface was modified with chitosan click-coupled dendrimer polyamide-amine (CS-PAMAM) as NO donor, and finally coated with polycaprolactone (PCL), and finally prepared a , the middle is chitosan grafted dendritic polyamide-amine, and the outermost layer is polycaprolactone. The composite membrane material and its application of controlled release of nitric oxide under alternating magnetic field have not been reported yet.

Method used

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  • Composite membrane material for releasing nitric oxide by magnetic control as well as preparation method and application of composite membrane material
  • Composite membrane material for releasing nitric oxide by magnetic control as well as preparation method and application of composite membrane material
  • Composite membrane material for releasing nitric oxide by magnetic control as well as preparation method and application of composite membrane material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] (1) Azide modified chitosan (CS-N 3 )Synthesis:

[0103] Azidoacetic acid (N 3 -CH2-COOH) was dissolved in N-N-dimethylformamide (DMF), and then sequentially added 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) and N-hydroxysuccinimide (NHS) activation 30min, obtains in the azidoacetic acid mixed solution; After the activation finishes, chitosan (the molecular weight of CS is 2000, deacetylation degree 85%) is dissolved in pure water, then slowly adds into In the above mixed solution of azidoacetic acid, react at 35°C for 12h. The product was dialyzed in deionized water for 2 days, freeze-dried to obtain the product chitosan azide; wherein, the chitosan, 1-ethyl-(3-dimethylaminopropyl) carbodiimide salt The molar ratio of N,N-dimethylformamide (EDC·HCl), N-hydroxysuccinimide (NHS) and azidoacetic acid is 1:1.5:1.5:2; Add 5g azido acid meter in; Described pure water adds 5g chitosan in every 10ml.

[0104] (2) Synthesis of alkyne-containing pol...

Embodiment 2

[0107] (1) Azide modified chitosan (CS-N 3 )Synthesis:

[0108] Azidoacetic acid (N 3 -CH2-COOH) was dissolved in N-N-dimethylformamide (DMF), and then sequentially added 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) and N-hydroxysuccinimide (NHS) was activated for 4h to obtain azidoacetic acid mixed solution; after the activation, chitosan (the molecular weight of CS was 20000, and the degree of deacetylation was 40%) was dissolved in pure water, and then slowly added to In the above mixed solution of azidoacetic acid, react at room temperature 25°C for 24h. The product was dialyzed in deionized water for 3 days, and freeze-dried to obtain the product azide chitosan. Among them, the chitosan, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), N-hydroxysuccinimide (NHS) and azidoacetic acid The molar ratio is 1:10:10:10; the N,N-dimethylformamide is based on the addition of 1g azide per 10ml; the pure water is based on the addition...

Embodiment 3

[0112] (1) Azide modified chitosan (CS-N 3 )Synthesis:

[0113] Azidoacetic acid (N 3 -CH2-COOH) was dissolved in N-N-dimethylformamide (DMF), and then sequentially added 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) and N-hydroxysuccinimide (NHS) was activated for 2h to obtain azidoacetic acid mixed solution; after the activation, chitosan (the molecular weight of CS was 10000, and the degree of deacetylation was 60%) was dissolved in pure water, and then slowly added to In the above mixed solution of azidoacetic acid, react at room temperature at 5°C for 16h. The product was dialyzed in pure water for 2 days, and freeze-dried to obtain the product azide chitosan. Among them, the chitosan, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), N-hydroxysuccinimide (NHS) and azidoacetic acid The molar ratio is 1:5:5:5; the N,N-dimethylformamide is calculated by adding 3g azide in every 10ml; the pure water is added with 3g chitosan in ...

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Abstract

The invention discloses a composite membrane material for releasing nitric oxide by magnetic control as well as a preparation method and application of the composite membrane material. The preparationmethod of the material comprises the following steps of performing azidation modification on chitosan, then synthesizing chitosan coupling dendritic polyamidoamine (CS-PAMAM) by the chitosan and alkynyl group containing dendritic polyamidoamine with click reaction, then modifying the CS-PAMAM on the surface of a ferroferric oxide bare sphere, and finally loading nitric oxide on the modified CS-PAMAM to obtain the composite membrane material for releasing the nitric oxide by magnetic control. The composite membrane material for releasing the nitric oxide by magnetic control is wrapped with polycaprolactone, finally, a composite membrane antibacterial material for releasing the nitric oxide under alternating magnetic field is obtained, and the obtained antibacterial material has the advantages of uniformity in grain size, sensitivity in response, large NO loading and storage quantity, long releasing time, good biocompatibility, obvious antibacterial effect, safety, high efficiency, environmental protection and the like, and can be used for the field of biomedical engineering materials.

Description

technical field [0001] The invention belongs to the field of biomedical engineering materials, and in particular relates to a composite film material for releasing nitric oxide by magnetic control and its preparation method and application. Background technique [0002] Since Furchgott et al. discovered in 1980 that vascular endothelial cells can synthesize and secrete vascular endothelial-derived relaxing factor (EDRF), and in 1986 Furchgott and Ignarro independently proved that EDRF is NO, the basic and clinical research on NO has been vigorously developed, and NO has become One of the most compelling biomolecules of recent years. A large number of studies have shown that NO is an important biological messenger molecule, involved in vascular regulation, neurotransmission, inflammation and immune response, etc. It is widely distributed throughout the brain, blood vessels, immunity, lungs, reproductive organs and other organs. [0003] In recent years, it has been found tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08L67/04C08L87/00C08K3/22C08K3/28C08G81/00C08B37/08A61K47/69A61K47/61A61K33/00A61K41/00A61P31/04A61P17/02A61P29/00
CPCA61K9/0009A61K33/00A61K41/00C08B37/003C08G81/00C08J5/18C08J2367/04C08J2487/00C08K3/28C08K2003/2275
Inventor 马栋李国巍张武薛巍
Owner JINAN UNIVERSITY
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