High-throughput sequencing detection method used for HPV typing and integration

Abstract

The invention discloses a HPV typing and integrated high-throughput sequencing detection method. The method selects the genes of the current HPV subtypes and combines the second-generation high-throughput sequencing technology to more comprehensively detect the type of HPV infection in patients. It overcomes the difficulties of low accuracy rate, high false positive rate, poor repeatability and high missed diagnosis rate of traditional detection methods. In the field of molecular diagnosis, the most direct and clear technology is gene sequencing. Compared with the current classic Sanger sequencing method, the second-generation high-throughput sequencing technology has higher detection throughput, faster sequencing speed, and higher accuracy. Advantages such as lower cost and richer information. With the help of the second-generation high-throughput sequencing technology, this method can accurately type high-risk HPV and low-risk HPV and detect whether it has been integrated with the human genome, and accurately and individually evaluate the tester to prevent The risk of lesions, thereby preventing the occurrence of tumors.

Description

technical field [0001] The invention relates to a high-throughput sequencing detection method for HPV typing and integration. Background technique [0002] Human papillomavirus (HPV) is a small DNA virus that is highly specific for mucous membranes and epithelial skin. HPV virus is mainly composed of DNA core and protein capsid. Its genome is a double-stranded circular DNA, about 7.5-8.0 kb in length, containing 8 open reading frames (ORFs), divided into 3 regions according to different functions: (1) early region (early region, E region) : Encode six early proteins including E1, E2, E4, E5, E6, and E7 respectively, which are involved in viral DNA replication, transcription, translation regulation, and transformation; (2) late region (L region): encodes the main Coat protein L1 and minor coat protein L2; (3) non-coding region (uncoding region, UCR), also known as long control region (LCR) or upstream regulatory region (URR): contains the replication origin of HPV genomic D...

AI Technical Summary

Problems solved by technology

Laboratory technicians will observe abnormal cells under a microscope to diagnose the patient's condition. However, since this method only uses observation as the basis for diagnosis, its accuracy is low and false due to the influence of material collection, smear production quality, and film reading technology. High negative, poor repeatability, the result and analysis missed diagnosis rate can reach 30%

Among the genotyping methods for checking and screening HPV, real-time fluorescent PCR method and hybridization capture method are the most commonly used methods, but the diagnostic operation for different genotypes of HPV is cumbersome, and the detection throughput is low, which is difficult to meet the requirements of clinical simultaneous detection of multiple subtypes. Type needs, not suitable for large-scale screening of cervical cancer

Commercial hybrid capture kits

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