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Method for Improving Tobacco Leaf Quality by Utilizing Bacillus burrowing Bacillus

A coccus and cave technology, applied in the field of tobacco alcohol fermentation, can solve problems such as no public reports of Bacillus cavernosa

Active Publication Date: 2019-12-13
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention isolates a kind of Bacillus cavernosa from nature for the first time, and it is identified as a new species of microorganism. At present, there is no public report about the Bacillus cavernosa and its use

Method used

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  • Method for Improving Tobacco Leaf Quality by Utilizing Bacillus burrowing Bacillus
  • Method for Improving Tobacco Leaf Quality by Utilizing Bacillus burrowing Bacillus
  • Method for Improving Tobacco Leaf Quality by Utilizing Bacillus burrowing Bacillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] ——Application of Bacillus burrowing bacteria SYSUZL-2 in improving the quality of tobacco leaves, the specific steps are as follows:

[0024] (1) Slant culture: medium: glucose 0.5g; yeast extract 0.5g; peptone 0.5g; acid hydrolyzed casein 0.5g; soluble starch 0.5g; sodium pyruvate 0.3g; g; agar 15g; distilled water 1000ml, pH7.2; culture temperature is 28°C, culture time is 48h.

[0025] (2) Seed culture: pick part of the mycelium from the slope and insert it into the seed solution, and shake the flask for culture. Seed medium: dextrin 120g; soybean powder 40g; yeast extract 2g; tryptophan 0.5g; β-alanine 5g; magnesium sulfate 0.5g; ammonium phosphate 0.2g; ℃, and the incubation time was 36 hours.

[0026] (3) Fermentation culture: Inoculate into the seed medium according to 10% inoculum amount, shake flask culture. Culture medium: 10g soybean powder; 10g glucose; 3g peptone; 2.5g sodium chloride; .

[0027] (4) Take 1L of the fermentation culture solution, centri...

Embodiment 2

[0044] Repeat implementation 1, with the following differences: the treatment concentration is 20 mL per 1000 grams of shredded tobacco.

[0045] ——Chemical Composition Determination Results

[0046] The chemical composition test results of tobacco leaves are as follows (see Table 4, 5 for details). The experimental results show that, from the perspective of a single compound, obvious changes have taken place in the internal chemical composition of tobacco leaves treated with bacteria agents. Compared with the control, the content of total sugar and reducing sugar increased greatly, and the content of total nitrogen, cellulose, protein and nicotine decreased compared with the control. Sugar-to-nitrogen ratio, sugar-to-base ratio, and nitrogen-to-base ratio all improved. The systematic test data shows that all kinds of shredded tobacco treated with bacterial agents are better than the control, and each component in the shredded tobacco tends to be balanced. It is beneficial t...

Embodiment 3

[0058] Repeat implementation 1, with the following differences: the treatment concentration is 30 mL per 1000 grams of shredded tobacco.

[0059] ——Chemical Composition Determination Results

[0060] The chemical composition test results of tobacco leaves are as follows (see Table 7, 8 for details). The experimental results show that, from the perspective of a single compound, obvious changes have taken place in the internal chemical composition of tobacco leaves treated with bacteria agents. Compared with the control, the content of total sugar and reducing sugar increased greatly, and the content of total nitrogen, cellulose, protein and nicotine decreased compared with the control. Sugar-to-nitrogen ratio, sugar-to-base ratio, and nitrogen-to-base ratio all improved. The systematic test data shows that all kinds of shredded tobacco treated with bacterial agents are better than the control, and each component in the shredded tobacco tends to be balanced. It is beneficial t...

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Abstract

The invention discloses a method for improving the quality of tobacco leaves by using Bacillus burrowing bacteria. The strain used was classified as Blastococcus cavernae sp, which was preserved in the National Center for Type Culture Collection CCTCC on December 9, 2016, with the preservation number M2016739. The microbiological characteristics analysis and 16S rDNA gene sequence analysis showed that the strain was a new bacterium. Spraying SYSUZL-2 bacterial agent on tobacco leaves and then undergoing alcohol fermentation can increase the aroma quality and reducing sugar content of tobacco leaves, and reduce the content of protein, cellulose, total nitrogen and nicotine, and the ratio of sugar to nitrogen, sugar to alkali and nitrogen to alkali The ratio tends to increase, which makes the internal chemical components of tobacco leaves more harmonious, and the sensory quality is also significantly improved.

Description

technical field [0001] The invention belongs to the technical field of tobacco alcoholization and fermentation, and in particular relates to a method for improving the quality of tobacco leaves by using a newly discovered bacterium (Bacillus burrowing bacteria). Background technique [0002] Tobacco is one of the important pillars of our country's finances and has made great contributions to our country's social and economic development. In order to promote the progress of tobacco in our country and realize the sustainable development of the cigarette industry, we must take the road of tobacco technology innovation. The quality of tobacco leaves directly affects the intrinsic taste of cigarettes. The State Tobacco Monopoly Administration clearly pointed out that it is necessary to "overcome the key technologies that affect the aroma and quality of tobacco leaves" and narrow the quality gap with foreign tobacco leaves in order to improve domestic and foreign market competiti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01A24B3/12A24B5/00A24B15/28
CPCA24B3/12A24B5/00A24B15/28C12N1/20C12N1/205C12R2001/01
Inventor 陈兴段焰青刘秀明李文均党立志焦俊李智宇郭青
Owner CHINA TOBACCO YUNNAN IND
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