Unlock instant, AI-driven research and patent intelligence for your innovation.

Hollow mesoporous nano carbon sphere immobilized enzyme and preparation method thereof

A technology of nano-carbon spheres and immobilized enzymes, which is applied in the direction of immobilization on or in inorganic carriers, can solve the problems of difficult entry of enzymes into pores, easy leakage of enzymes, and insufficient stability, and avoid friction and shear force. Effects of damage and leakage, improved immobilization and stability, improved stability and activity

Active Publication Date: 2021-04-06
OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Carriers such as silica gel directly adsorb and immobilize the enzyme, which is not stable, and the enzyme is easy to fall off, requiring complex surface modification; the pore size should match the size of the enzyme, too large will easily cause the loss of the enzyme, and too small will make it difficult for the enzyme to enter inside the pores, resulting in low solid loading
Patent ZL201110350307.0 discloses a method for immobilizing enzymes on attapulgite, but complex silanization modification on the surface of attapulgite is required, and the enzyme immobilization capacity is not high; patent 102443579A discloses a method for applying amphiphilic A method for preparing immobilized enzymes from porous hollow carbon microspheres. The precursor of the hollow carbon material is yeast, and the size of the carrier material formed by carbonization of yeast is micron-scale, and the specific surface area is not large, resulting in a low enzyme immobilization capacity (<30.23mg / g ), the pore size is greater than 50nm, causing the enzyme to leak easily during use, and the stability is not high enough

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hollow mesoporous nano carbon sphere immobilized enzyme and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A hollow mesoporous nano-carbon sphere immobilized enzyme and a preparation method thereof, comprising the following steps:

[0036] 1) Silica / resorcinol-formaldehyde oligomer (SiO 2 Preparation of @RF): Disperse 96 mmol (27.68 mL) of tetrapropoxysilane into 640 mL of ethanol / water reaction solvent with a volume ratio of 7:1, add 24 mL of 25% to 28% concentrated ammonia as a catalyst, and stir for 20 min , then add 3.2g resorcinol and 4.48mL formaldehyde, stir and react at 25°C for 24h, after the reaction, wash with distilled water and ethanol three times in turn, and dry at 50°C to obtain silica / resorcinol-formaldehyde oligomerization matter (SiO 2 @RF);

[0037] 2) Silicon dioxide / carbon (SiO 2 @C) Preparation of the composite: SiO obtained after drying 2 @RF was placed in a tube furnace, with high-purity nitrogen as a protective gas, raised from room temperature to 600 °C at a rate of 5 °C / min, and calcined at 600 °C for 5 h to obtain a silica / carbon composite ( ...

Embodiment 2

[0043] A hollow mesoporous nano-carbon sphere immobilized enzyme and a preparation method thereof, comprising the following steps:

[0044] 1) Silica / resorcinol-formaldehyde oligomer (SiO 2 Preparation of @RF): Disperse 96 mmol (21.00 mL) of tetraethoxysilane into 640 mL of ethanol / water reaction solvent with a volume ratio of 2:1, add 20 mL of 25% to 28% concentrated ammonia as a catalyst, and stir for 20 min , then add 3.2g resorcinol and 4.48mL formaldehyde, stir and react at 25°C for 24h, after the reaction, wash with distilled water and ethanol three times in turn, and dry at 50°C to obtain silica / resorcinol-formaldehyde oligomerization matter (SiO 2 @RF);

[0045] 2) Silicon dioxide / carbon (SiO 2 @C) Preparation: SiO obtained after drying 2 @RF was placed in a tube furnace, with high-purity nitrogen as a protective gas, raised from room temperature to 800 °C at a rate of 5 °C / min, and calcined at 800 °C for 5 h to obtain a silica / carbon composite ( SiO 2 @C);

[0...

Embodiment 3

[0050] A hollow mesoporous nano-carbon sphere immobilized enzyme and a preparation method thereof, comprising the following steps:

[0051] 1) Silica / resorcinol-formaldehyde oligomer (SiO 2 Preparation of @RF): Disperse 24mmol (5.25mL) of tetraethoxysilane and 72mmol (20.76mL) of tetrapropoxysilane into 640mL of ethanol / water reaction solvent with a volume ratio of 3:1, add 32mL, 25% to 28% concentrated ammonia water was used as a catalyst, stirred for 20 minutes, then added 3.2g resorcinol and 4.48mL formaldehyde, stirred and reacted at 25°C for 24h, after the reaction was completed, washed three times with distilled water and ethanol in sequence, and dried at 50°C to obtain Silica / resorcinol-formaldehyde oligomer (SiO 2 @RF);

[0052] 2) Silicon dioxide / carbon (SiO 2 @C) Preparation: SiO obtained after drying 2 @RF was placed in a tube furnace, with high-purity nitrogen as a protective gas, raised from room temperature to 700 °C at a rate of 5 °C / min, and calcined at 700...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
thicknessaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses an enzyme immobilized by hollow mesoporous nanocarbon spheres and a preparation method thereof. The enzyme immobilized by hollow mesoporous nanocarbon spheres comprises an enzyme and a carrier hollow mesoporous nanocarbon spheres for immobilizing the enzyme. The particle diameter of the hollow mesoporous carbon nanospheres described above is 300-400nm, and the specific surface area is 1100-1200m 2 / g, shell thickness is 20~60nm, cavity size is 250~350nm, and the aperture size of mesopore is 8~14nm; Its preparation method comprises the following steps: 1) silicon dioxide / resorcinol-formaldehyde oligomer (SiO 2 Preparation of @RF); 2) Silica / Carbon (SiO 2 @C) preparation; 3) preparation of hollow mesoporous carbon nanospheres; 4) immobilization of enzymes on hollow mesoporous carbon nanospheres. The physical structure and hydrophobic surface of the hollow mesoporous nano-carbon sphere immobilized enzyme in the present invention can greatly improve the immobilized capacity and stability of the enzyme, which is conducive to the exposure of the active site of the enzyme, and is more efficient than conventional adsorption materials. It is beneficial to the adsorption, immobilization and activity maintenance of enzymes.

Description

technical field [0001] The invention belongs to the technical field of enzyme immobilization, and relates to an enzyme immobilized by hollow mesoporous nanometer carbon spheres and a preparation method thereof. Background technique [0002] Enzymes are green and efficient biocatalysts. However, the free enzyme has poor stability, low activity, and is difficult to recycle and reuse, which greatly limits its application in industry. Enzyme immobilization refers to a technology that limits natural free enzymes to a certain space and cannot move freely, which greatly improves the catalytic activity and stability of enzymes. At present, immobilized enzymes have been widely used in food, medicine, energy, environment and other fields. The carrier of immobilized enzyme is the main factor affecting its performance. Commonly used supports include porous silicon materials, macroporous resins, molecular sieves, etc. Carriers such as silica gel directly adsorb and immobilize the enz...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14
CPCC12N11/14
Inventor 郑明明黄凤洪董喆时杰汤虎邓乾春
Owner OIL CROPS RES INST CHINESE ACAD OF AGRI SCI