Preparation method of nano-gold immobilized beads and its application in thiram detection

A technology of immobilizing small balls and nano-gold, which is applied in the direction of analysis by chemical reaction of materials and material analysis by observing the influence of chemical indicators, which can solve the problems of limited repeatability and reliability of test results, and test results. Problems such as poor repeatability and reliability, poor stability of the detection system, etc., to achieve the effect of easy on-site detection, improved storage stability, and less sample volume
CN107764816BActive Publication Date: 2019-12-24CHINA UNIV OF PETROLEUM (EAST CHINA)
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA UNIV OF PETROLEUM (EAST CHINA)
Publication Date
2019-12-24

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Abstract

The invention provides a preparation method of nano gold immobilized pellets and application of the nano gold immobilized pellets to ziram detection. The preparation method comprises the following steps: weighing ditert-butyl bicarbonate, NHS and EDC, adding the ditert-butyl bicarbonate, the NHS and the EDC into an MES buffer solution for stirring reaction, adding a chitosan solution for reactionover night, and placing the solution in an ultrafiltration pipe for centrifugal ultrafiltration, thereby obtaining a chitosan grafted tyrosine solution; then adding a chloroaurate aqueous solution into the chitosan grafted tyrosine solution, adjusting the pH value, uniformly shaking the mixed solution, and stewing to obtain a chitosan-nano gold solution; mixing the obtained chitosan-nano gold solution with epoxy chloropropane, dropwise adding the mixture into a sodium hydroxide solution, filtering out suspended pellets in the solution, and drying the pellets, thereby obtaining the nano gold immobilized pellets. The nano gold immobilized pellets prepared by the preparation method provided by the invention are easy to store and high in stability, do not need to be prepared for instant use, and is convenient to use; when the nano gold immobilized pellets are applied to the ziram detection, a detection system is high in stability, and a detection result is high in repetitiveness and reliability.
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Description

technical field

[0001] The invention belongs to the field of pesticide residue detection, and in particular relates to a preparation method of nano-gold immobilized balls and its application in thiram detection. Background technique

[0002] Zirconia dithiocarbamate (zinc dimethyl dithiocarbamate) is a dithiocarbamate fungicide that can prevent and treat various fungal plant diseases. It has high efficiency, low toxicity, and is not easy to induce pathogen resistance. It is relatively safe for humans, animals and plants, and has a broad-spectrum bactericidal effect on plant diseases, and is widely used in agricultural production. However, zirconium moves quickly in the soil and easily enters groundwater, and its metabolites (ethylenethiourea and propylenethiourea) also have carcinogenic, mutagenic and teratogenic effects. Therefore, the detection of ziram should be strengthened very important.

[0003] At present, traditional methods such as chromatography and capillary el...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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