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Application of rice nrt1.1a gene and its encoded protein in breeding for improving plant yield

A technology of transgenic plants and plants, applied in plant peptides, genetic engineering, recombinant DNA technology, etc., can solve problems such as environmental degradation, excessive use of nitrogen fertilizer, and low nitrogen use efficiency

Active Publication Date: 2020-04-28
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are a series of problems in my country, such as excessive use of nitrogen fertilizer, low nitrogen use efficiency and environmental degradation caused by nitrogen fertilizer loss.

Method used

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  • Application of rice nrt1.1a gene and its encoded protein in breeding for improving plant yield
  • Application of rice nrt1.1a gene and its encoded protein in breeding for improving plant yield
  • Application of rice nrt1.1a gene and its encoded protein in breeding for improving plant yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1, Analysis of the expression of NRT1.1A in rice tissues

[0078] The NRT1.1A gene involved in this example is derived from rice (Oryza.sativa L.), its genome sequence is shown in sequence 2 in the sequence listing, sequence 2 consists of 8143 nucleotides, and its cDNA sequence is the sequence listing Middle sequence 3, sequence 3 consists of 1812 nucleotides. Sequence 2 and Sequence 3 encode the protein (NRT1.1A protein) shown in Sequence 1 in the Sequence Listing, and Sequence 1 consists of 603 amino acid residues.

[0079] Total RNA was extracted from the root, stem, leaf sheath, leaf and ear of rice variety Dongjin and cDNA was obtained by reverse transcription. Furthermore, using the obtained cDNA as a template, real-time quantitative fluorescent PCR was carried out for the NRT1.1A gene to detect the expression level of the NRT1.1A gene at the transcription level in various tissues of rice. The experiment was repeated 3 times, and the results were averaged....

Embodiment 2

[0088] Example 2. Verification of NRT1.1A's in vitro nitrate transport activity

[0089] The NRT1.1A gene involved in this example is derived from rice (Oryza.sativa L.), its genome sequence is shown in sequence 2 in the sequence listing, sequence 2 consists of 8143 nucleotides, and its cDNA sequence is the sequence listing Middle sequence 3, sequence 3 consists of 1812 nucleotides. Sequence 2 and Sequence 3 encode the protein (NRT1.1A protein) shown in Sequence 1 in the Sequence Listing, and Sequence 1 consists of 603 amino acid residues.

[0090] 1. Xenopus laevis recombinant expression vector pCS2 + / Build of NRT1.1A

[0091] The total RNA of japonica rice variety Dongjin was extracted and reverse transcribed into cDNA. The obtained cDNA was used as a template, and the NRT1.1A cDNA was amplified by PCR using the following primer sequences. The two ends of the primers used for amplification were respectively introduced into the recognition sites of the restriction endonu...

Embodiment 3

[0101] Example 3. Obtainment and functional verification of rice nrt1.1a mutant

[0102] The NRT1.1A gene involved in this example is derived from rice (Oryza.sativa L.), its genome sequence is shown in sequence 2 in the sequence listing, sequence 2 consists of 8143 nucleotides, and its cDNA sequence is the sequence listing Middle sequence 3, sequence 3 consists of 1812 nucleotides. Sequence 2 and Sequence 3 encode the protein (NRT1.1A protein) shown in Sequence 1 in the Sequence Listing, and Sequence 1 consists of 603 amino acid residues.

[0103] 1. Obtaining and identification of rice nrt1.1a mutant

[0104] Rice nrt1.1a mutant: from Korean Rice Mutant Bank (Crop Biotech Institute, KyungHee University, Republic of Korea, http: / / www.postech.ac.kr / life / pfg / risd), the order URL is http: / / signal.salk.edu / cgi-bin / RiceGE5, the corresponding number of the rice nrt1.1a mutant is PFG_1E-00433.L. The specific construction method is described in the article "Jakyung Yi, Gynheung A...

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Abstract

The invention discloses the application of a rice NRT1.1A gene and its coded protein in breeding for improving plant yield. The application provided by the present invention is specifically the application of the protein or its coding gene in regulating plant growth and development; the growth and development is reflected in the yield per plant and / or plant height and / or grain number per ear and / or flowering time and / or Bolting time and / or rosette leaf size; the protein is any of the following: (1) the amino acid sequence is the protein shown in sequence 1 in the sequence listing; (2) the amino acid sequence of sequence 1 in the sequence listing is passed through one or A protein derived from Sequence 1 that has several amino acid residues substituted and / or deleted and / or added and is related to the regulation of plant growth and development. The invention has great significance for cultivating new rice varieties with high yield.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a rice NRT1.1A gene and its coded protein in breeding for improving plant yield. Background technique [0002] Plants need a variety of mineral nutrients to maintain their normal growth, and nitrogen is the mineral nutrient element that plants need the most. Generally speaking, nitrogen can account for 1.5-2% of the dry weight of the plant body. Nitrogen is a constituent of proteins, nucleic acids, phospholipids, and organic nitrogen compounds necessary for plant growth and development, and these substances are structural or functional components for living cells to survive, so nitrogen is also called a life element. In order to pursue the high yield of crops, a large amount of nitrogen fertilizer is often applied in agricultural production. Among them, the amount of nitrogen fertilizer applied to rice far exceeds that of any other crops, and the loss of nitrogen f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82A01H6/46A01H6/20
CPCC07K14/415C12N15/8243C12N15/8261C12N15/82Y02A40/146
Inventor 储成才王威胡斌李华张志华刘永强
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI