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A kind of mucopolysaccharide detection kit

A technology for detecting kits and mucopolysaccharides, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by causing materials to undergo chemical reactions. It can solve the problem that colorimetry cannot be directly quantified and electrophoresis is complicated , the need for instruments and other issues, to achieve the effect of easy observation, fast and simple detection method, and convenient portability

Active Publication Date: 2020-07-24
SHAANXI HUIKANG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have many disadvantages, such as colorimetric method cannot be directly quantified, the accuracy is relatively poor, the operation process of electrophoresis method is complicated, requires instruments, cannot be quantified, and the cost is relatively high

Method used

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  • A kind of mucopolysaccharide detection kit

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Effect test

Embodiment 1

[0017] The mucopolysaccharide detection kit of this embodiment consists of four parts: a chromogenic plate, a chromogenic solution, an eluent, and an elution tank. The chromogenic solution is an aqueous solution of acetic acid dissolved with Alician blue, wherein the concentration of Alician blue is 1.2 mg / mL, and the mass fraction of acetic acid is 2%. The eluent is an aqueous solution of acetic acid with a mass fraction of 12%. The chromogenic plate includes a base plate bracket and a sampling filter paper. The sampling filter paper is a filter paper with good adsorption properties, and its periphery is fixed on the base plate bracket by clips. A standard control area and a sample detection area are set on the sampling filter paper. The preparation method of the standard control area is as follows: add the chondroitin sulfate standard to deionized water, and prepare concentrations of 2 μg / μL, 1 μg / μL, and 0.5 μg / μL, respectively. μL, 0.25 μg / μL, 0.10 μg / μL, 0.05 μg / μL, 0 μg...

Embodiment 2

[0020] In this embodiment, the chromogenic solution is a formic acid-Tris aqueous solution in which 1,9-dimethylmethylene blue is dissolved, wherein the concentration of 1,9-dimethylarthalene blue is 1.4 mg / mL, and the concentration of formic acid is 50mmol / L, the concentration of Tris is 0.2mol / L, the pH of the chromogenic solution is 9.0; the eluent is a mixture of 50mmol / L formic acid aqueous solution and absolute ethanol with a volume ratio of 70:30; the preparation of the standard control area The method is: add the chondroitin sulfate standard substance into deionized water, and prepare respectively Chondroitin sulfate standard solution, take 5 μL of chondroitin sulfate standard solution of different concentrations and evenly drop it on different areas of the sampling filter paper, dry it with a hair dryer, and then drop it on each area where the chondroitin sulfate standard solution is dropped Add the chromogenic solution, react for 1 minute, shake off the chromogenic s...

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Abstract

The invention discloses a mucopolysaccharide detection kit, which comprises a developing plate, developing solution and eluent; the developing plate comprises a bottom plate tray and a sampling filterpaper; a standard product contrast zone and a sample detection zone are arranged on the sampling filter paper; the developing solution is acetic acid aqueous solution dissolved with Alcian blue or methyl-Tris aqueous solution dissolved with 1, 9- dimethyl methylene blue, wherein when the developing solution is the acetic acid aqueous solution dissolved with Alcian blue, the eluent is 5%-15% by mass of acetic acid aqueous solution; when the developing solution is the methyl-Tris aqueous solution dissolved with 1, 9- dimethyl methylene blue, the eluent is the mixed fluid of 50 mmol / L methanoicsolution and absolute ethyl alcohol at volume ratio of 75: 25-65: 35. The mucopolysaccharide detection kit is simple in structure, good in specificity, and can quantificationally test the mucopolysaccharide content in the urine; the detection sensitivity and efficiency are high, the identification result is exact, the operation is simple, and the detection cost is low; the invention provides a fast and simple detection method for mucopolysaccharide disease.

Description

technical field [0001] The invention belongs to the technical field of biochemical detection, and in particular relates to a mucopolysaccharide detection kit. Background technique [0002] Mucopolysaccharide etiology Proteoglycan breakdown disorders caused by congenital defects in proteoglycan-degrading enzymes. It is characterized by excessive accumulation and excretion of oligosaccharides, resulting in bone development disorders, hepatosplenomegaly, mental retardation, and increased excretion of mucopolysaccharides in urine. Mucopolysaccharide is a nitrogen-containing heterogeneous polysaccharide, which is the main component of the intercellular connective tissue and widely exists in various cells of mammals. Its chemical composition is alternate appearance of uronic acid and tyrosyl hexose, sometimes containing sulfur bonds , also known as glycosaminoglycans. [0003] At present, the detection methods of mucopolysaccharide mainly include: (1) colorimetric method: DMB-Tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 王鑫张凤龙高恩赵金礼
Owner SHAANXI HUIKANG BIO TECH CO LTD