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A method and application of rapid and efficient extraction of antibiotics and their metabolites in plant samples

A technology for antibiotics and metabolites, which is applied in the field of rapid and efficient extraction of antibiotics and their metabolites from plant samples, can solve the problems of great influence on the accuracy of test results, high proficiency requirements, and limited purification effects, and achieves easy standardization. And combined with instrument detection, good extraction efficiency, stable repeatability

Active Publication Date: 2020-12-22
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the research progress, it is reported that the extraction method adopts the combination of liquid-liquid extraction and solid-phase extraction column purification, but the requirement for the proficiency of the experimental operator is high, the repeatability is unstable, and the processing time is still relatively long
In the purification process, PSA is used more often as a purifier. PSA has a certain ability to remove pigment, but it is relatively weak. After purification, there are still a lot of pigment and other impurities on the machine, especially for some plant samples. There are limitations in its purification effect, which has a great impact on the accuracy of the test results

Method used

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  • A method and application of rapid and efficient extraction of antibiotics and their metabolites in plant samples
  • A method and application of rapid and efficient extraction of antibiotics and their metabolites in plant samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042]Example 1 Extraction and recovery rate of sulfonamide antibiotics and their metabolites in water spinach and canna

[0043]Instruments and reagents used in the embodiments of the present invention:

[0044]Ultra-high liquid chromatography-mass spectrometer (Agilent Technologies, US), high-speed refrigerated centrifuge, vortex, nitrogen blower, and electronic balance are all commonly used equipment in this field.

[0045]Reagents: methanol (HPLC), acetonitrile, acetic acid, NaCl, Na3C6H5O7• 2H2O), C6H6Na2O7·1.5H2O, MgSO4, GCB, C18 and PSA powder are all conventionally purchased commercially. Among them, magnesium sulfate needs to be dried in a muffle furnace in advance for use.

[0046]Sulfamethoxazole (SMX), Sulfamethoxazole (SMZ2) and N4Acetyl metabolites (Ac-SMX, Ac-SMZ2) standard products (purity> 98%) were purchased from Sigma.

[0047]S1. Sample pretreatment:

[0048]The roots and leaf samples of water spinach and canna that were not contaminated by sulfonamide antibiotics were collected a...

Embodiment 2

[0066]Example 2 Extraction and determination of sulfonamide antibiotics and their metabolites in plant samples

[0067]Plant collection: Collect vegetables and wetland plants that have been irrigated with domestic sewage for a long time. Take water spinach and canna as examples.

[0068]S1. Plant pretreatment: The collected plants are washed with ultrapure water and the surface dust is absorbed by absorbent paper. The samples are divided into underground roots and above-ground stems and leaves. The samples are freeze-dried and ground with a crusher and passed through a 60-mesh sieve.

[0069]S2. Extraction: Weigh 1.00g plant sample, add 0.2g NaCl and 0.2 gNa into a 50mL centrifuge tube with stopper3C6H5O7• 2H2O), 0.1g disodium citrate 1.5 hydrate (C6H6Na2O7·1.5H2O), 0.2g anhydrous magnesium sulfate (MgSO4), quickly vortex for 1min, centrifuge at 10000r / min for 10min, take the supernatant in another clean centrifuge tube, add 5mL acidified acetonitrile to the residue for the second extraction...

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Abstract

The invention discloses a method for fast and high-efficiency extraction of antibiotics and metabolites thereof in plant samples and an application thereof. Aiming at the plant samples, a solid-phaseextractant scientifically replaces liquid-liquid extraction and solid-phase extraction columns, and good extraction efficiency is ensured; a purifying agent is optimized further, the extraction and purification integrated sample extraction and purification method for high-efficiency extraction of the antibiotics in the plants is formed, and the method has the advantages of short required time, high extraction efficiency, stable repeatability, simple operation, time saving, high efficiency, high extraction and recovery rate and good reproducibility, and lays the foundation for follow-up detection accuracy.

Description

Technical field[0001]The invention relates to the technical field of physical and chemical detection of antibiotic residues in plants, and more specifically, to a method and application for quickly and efficiently extracting antibiotics and their metabolites from plant samples.Background technique[0002]Antibiotics are antibacterial and anti-inflammatory drugs. They are widely used in livestock and aquaculture. Antibiotics cannot be completely metabolized after being absorbed by animals and entering the body. Most of them are excreted in feces and urine in the form of mothers, leading to antibiotics. Continue to enter the environment, causing a pseudo-persistence of antibiotics in the environment.[0003]Antibiotics entering the environment will cause ecological toxic effects on plants, and the photosynthesis and growth rate of plants will be inhibited. Quantifying the enrichment of antibiotics and their metabolites in plants can clarify the consumption risk of plant food on the one ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/34
CPCG01N1/28G01N1/286G01N1/34
Inventor 杨扬杨瑜芬邰义萍王瑞王佳希万翔段杰翰
Owner JINAN UNIVERSITY