Preparation method of macadimia nut polypeptides
A technology for nuts and nut meal, applied in the field of macadamia nut polypeptide preparation, can solve the problems of protein resource waste and low utilization rate of macadamia nut meal, and achieve the effects of stable polypeptide properties, improved protein utilization rate, and low molecular weight
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Embodiment 1
[0016] Example 1 The preparation of a macadamia nut polypeptide comprises the following steps:
[0017] a, crushing: hydraulically pressed macadamia nut meal is crushed by a plant crusher and passed through a 60-mesh sieve to obtain macadamia nut meal powder;
[0018] b, the homogeneity of the suspension: add water to the pulverized macadamia nut meal powder and mix thoroughly, and obtain a uniform mixed solution through a homogenizer, and the homogenization pressure is 25MPa; the mixing mass ratio of macadamia nut meal powder and water is 1: 5;
[0019] c. Constant temperature enzymolysis: preheat the mixture in boiling water for 10 minutes, cool to 50°C and add neutral protease, the amount of enzyme added is 3200U / g, adjust the pH value to 7.0, and carry out constant temperature enzymolysis for 8 hours to obtain enzymolysis liquid;
[0020] d. Boiling water sterilization: heat the enzymatic solution in a boiling water bath for 5 minutes;
[0021] e. Centrifugal separation...
Embodiment 2
[0023] Example 2 The preparation of a macadamia nut polypeptide comprises the following steps:
[0024] a, crushing: hydraulically pressed macadamia nut meal is crushed by a plant crusher and passed through a 60-mesh sieve to obtain macadamia nut meal powder;
[0025] B, the homogeneity of suspension: add water to the pulverized macadamia nut meal powder and mix thoroughly, and homogenize through a homogenizer to obtain a uniform mixed solution, the homogenization pressure is 30MPa; the mixing mass ratio of macadamia nut meal powder and water is 1: 6;
[0026] c. Constant temperature enzymolysis: preheat the mixture in boiling water for 10 minutes, cool to 50°C and add neutral protease, the amount of enzyme added is 3200U / g, adjust the pH value to 8.5, and carry out constant temperature enzymolysis for 6 hours to obtain enzymolysis liquid;
[0027] d. Boiling water sterilization: heat the enzymatic solution in a boiling water bath for 8 minutes;
[0028] e. Centrifugal sepa...
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