A gene sequence of a human-derived keratinocyte growth factor-2, an expression vector and a production method

Abstract

A gene sequence of a human-derived keratinocyte growth factor-2, an expression vector and a production method are disclosed. Through designing the gene sequence, of the human-derived keratinocyte growth factor-2, meeting codon preference properties of a target plant, the suitable plant expression vector including the KGF-2 gene sequence is constructed, the plant expression vector is transferred tothe target plant, and multiple screening is performed to obtain a transgenic plant having a high protein expression level, wherein the target plant is medicago sativa. The production cost is greatlyreduced when the medicago sativa is utilized to express the human-derived KGF-2 than the production cost when microorganism expression systems are utilized to express the human-derived KGF-2, and protein products can be correctly folded to avoid generation of inclusion bodies. The gene sequence, the expression vector and the method have good application prospects.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a gene sequence, an expression vector and a production method of human keratinocyte growth factor-2. Background technique [0002] Keratinocyte growth factor-2 (KGF-2) is an important member of the fibroblast growth factor (Fibroblast Growth Factor, FGFs) family. In 1996, Yamasaki et al first reported a new type of cell regulatory factor isolated from rat embryos that is closely related to the growth of keratinocytes, named KGF-2. The following year, Emoto et al. cloned the complete cDNA of human KGF-2, which encodes a single-chain polypeptide protein factor with a full length of 208 amino acids containing a 40-amino acid signal peptide at the N-terminus. [0003] Through in-depth research on the mechanism of KGF-2, it is found that it interacts with two cell membrane surface receptors (FGFR2IIIb and FGFR1IIIb) to produce a regulatory effect, especially F...

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Problems solved by technology

[0006] Through a large number of studies, the present inventors provided a method for using specific plants as bioreactors to produce human KGF-2 protein, which solved the problems of high production cost and poor expression products in existing prokaryotic expression system technologies such as Escherichia coli. A series of problems such as inclusion bodies, poor solubility, and toxicity to host cells

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