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Bacillus thuringiensis microcapsule suspension for killing grubs and its preparation method

A microcapsule suspension, Bacillus aureus technology, applied in the direction of insecticides, botanical equipment and methods, biocides, etc., can solve the problems of unstable drug efficacy, poor environmental adaptability, etc., and achieve improved stability and UV resistance Ability to prolong the effect of the drug

Active Publication Date: 2021-04-02
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention aims at the shortcoming of present bacillus thuringiensis preparation, with the Bt new bacterial strain B-Y7-1 spore and insecticidal crystal protein having high-efficiency insecticidal activity to grub class pests as model drug, with polymer material gelatin, gum arabic as carrier, Chitin nanofibers are used as toughening agents to prepare microcapsule suspensions, which solve the outstanding problems of unstable efficacy and poor environmental adaptability of traditional Bt biopesticide preparations, and at the same time realize the slow release of preparation products, so as to provide new Bt strains for killing grub-like pests. The application and promotion of B-Y7-1 laid the foundation

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  • Bacillus thuringiensis microcapsule suspension for killing grubs and its preparation method

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Embodiment

[0022] 5% Bacillus thuringiensis microcapsule suspension for killing grubs

[0023] The components are calculated by weight ratio: Bacillus thuringiensis B-Y7-1 powder 5%, gelatin 2%, gum arabic 2%, melanin 2%, tristyryl polyoxyethylene ether phosphate 5%, polycarboxylic acid Salt 5%, xanthan gum 0.2%, propylene glycol 5%, sodium benzoate 0.2%, water to make up to 100%.

[0024] Preparation:

[0025] (1) Take 5g of Bacillus thuringiensis powder, 2g of melanin and 5% Tween-80 aqueous solution and stir evenly to prepare a bacterial suspension;

[0026] (2) Add 1 g of chitin nanofibers into distilled water, stir at room temperature for 1 hour, then grind for 10 minutes, and then ultrasonically disperse for 30 minutes to prepare a suspension of chitin nanofibers;

[0027] (3) adding the chitin nanofiber suspension prepared in step (2) to the bacterial suspension prepared in step (1), stirring continuously for 1 hour, standing for degassing, and obtaining a suspension mixing syst...

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Abstract

The invention provides grub-pest-killing Bacillus thuringiensis microcapsule suspension and a preparation method thereof. Raw powder of novel grub-pest-killing Bacillus thuringiensis strain B-Y7-1 isused as an effective ingredient; gelatin and Arabic gum are used as support materials; chitin nano fiber is used as a toughener; formaldehyde is added for curing, and mixing with a dispersing wettingagent, the thickener, an anti-freezing agent and a preservative is performed to prepare the stable microcapsule suspension. The microcapsule suspension of the invention has encapsulation rate of 85% and above and average particle size of less than 10 mu m; the microcapsule suspension has stable properties and good anti-ultraviolet capacity, has good preventive effect and lasting action for preventing and treating grub pests in agricultural production and has a promising application and popularization prospect.

Description

technical field [0001] The invention relates to a microcapsule suspension and a preparation method of a new bacillus thuringiensis strain B-Y7-1 having high-efficiency insecticidal activity against grub-type pests, and belongs to the technical field of biological pesticides. Background technique [0002] Bacillus thuringiensis (Bt) is the most studied and widely used microbial pesticide in the world, which is safe for humans and animals and does not destroy the ecological balance. Bacillus thuringiensis has significant advantages as a biological insecticide, but because the main insecticidal component of Bt is insecticidal crystal protein, the ability to resist ultraviolet rays and adverse environmental conditions is poor, resulting in the short duration of Bacillus thuringiensis preparations, and the control effect is difficult. Restricted by sunlight, temperature, rain and other external climate and ecological conditions, the short residual period of field application is t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/23A01N25/28A01N25/22A01N25/04A01P7/04
CPCA01N25/04A01N25/22A01N25/28A01N63/00
Inventor 张保华李长友郑桂玲辛露露张雨
Owner QINGDAO AGRI UNIV