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Efficient animal tendon smashing method

An animal and powder technology, applied in the fields of biotechnology, daily chemical industry, and medical equipment, can solve the problems of easy pollution of the environment, cumbersome operation methods, and residual organic solvents, and achieve low labor intensity, no environmental pollution, and low production costs. Effect

Inactive Publication Date: 2018-04-17
浙江崇山生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the strong toughness of tendon tissue, it is difficult to slice and crush without special treatment, which directly affects the extraction efficiency of collagen
Since the Achilles tendon is derived from animals, there is a small amount of oil, which is easy to emulsify, which is not conducive to the purification of collagen
Acetone, ethanol, n-butanol can be used to soak and degrease, but the operation method is cumbersome, easy to pollute the environment, organic solvent residue and other defects

Method used

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  • Efficient animal tendon smashing method
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  • Efficient animal tendon smashing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The method for effectively pulverizing the beef Achilles tendon and removing fat from the beef Achilles tendon mainly comprises the following steps:

[0033] Step 1: After removing the mechanical fascia, muscle and fat of the bovine Achilles tendon, rinse it with water or sodium chloride solution, and freeze it.

[0034] Step 2: Take out the beef Achilles tendon after freezing and hardening, scrape off the residual fascia on the surface of the Achilles tendon with a blade, and cut the Achilles tendon into thin slices with a microtome.

[0035] Step 3: Roll the thin slices of beef Achilles tendon into strips, freeze and harden before cutting into thin slices;

[0036] Step 4: put the bovine Achilles tendon thin slices into a certain concentration of sodium chloride solution, and pulverize with a pulverizer or grinder to obtain the bovine Achilles tendon suspension.

[0037] Step 5, centrifuging the bovine Achilles tendon suspension, discarding the saline solution, washi...

Embodiment 2

[0040] The method for effectively crushing sheep Achilles tendon and removing fat from sheep Achilles tendon mainly includes the following steps:

[0041] Step 1: After removing the mechanical fascia, muscle and fat of the sheep Achilles tendon, rinse it with water or sodium chloride solution, and freeze it.

[0042] Step 2: Take out the sheep Achilles tendon after freezing and hardening, scrape off the residual fascia on the surface of the Achilles tendon with a blade, and cut the Achilles tendon into thin slices with a microtome.

[0043] Step 3: Roll the lamb Achilles tendon thin slices into strips, freeze and harden before cutting into thin slices;

[0044] Step 4: Put the sheep Achilles tendon thin slices into a certain concentration of sodium chloride solution, and pulverize with a pulverizer or grinder to obtain the sheep Achilles tendon suspension.

[0045] Step 5, centrifuging the sheep Achilles tendon suspension, discarding the salt solution, washing the residue wit...

Embodiment 3

[0047] The method for effectively crushing the Achilles tendon and removing grease from the Achilles tendon mainly includes the following steps:

[0048] Step 1: After removing the mechanical fascia, muscle and fat of the equine Achilles tendon, rinse with water or sodium chloride solution, and freeze.

[0049] Step 2: Take out the frozen and hardened Achilles tendon, scrape off the residual fascia on the surface of the Achilles tendon with a blade, and cut the Achilles tendon into thin slices with a microtome.

[0050] Step 3: Roll the horse Achilles tendon thin slices into strips, freeze and harden before cutting into thin slices;

[0051] Step 4: put the horse Achilles tendon thin slice into a certain concentration of sodium chloride solution, and pulverize it with a pulverizer or grinder to obtain the horse Achilles tendon suspension.

[0052] Step 5, centrifuging the horse Achilles tendon suspension, discarding the saline solution, washing the residue with water, and cen...

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Abstract

The invention discloses a simple and feasible achilles-tendon-type smashing and grease removing method. Achilles-tendon-type powder obtained by smashing through the method is suitable for extraction of collagen proteins, and the collagen proteins keep complete triple-helical structures and can be used for preparing collagen protein sponges and collagen protein related products. The method comprises the main steps that fascias, muscles and fat of animal achilles tendons rich in the collagen proteins are mechanically removed, and then the animal achilles tendons are rinsed through water or a sodium chloride solution and frozen; the frozen and hardened animal achilles tendons are taken out, the residual fascias on the surfaces of the achilles tendons are scraped through a blade, and the achilles tendons are cut into slices through a slicer; the achilles-tendon-type slices are each rolled into a strip shape, and the strip-shaped achilles-tendon-type slices are frozen, hardened and then cutinto slices; the achilles-tendon-type slices are placed into a sodium chloride solution with certain concentration and smashed through a smashing machine or a grinding machine, and an achilles-tendon-type suspension is obtained; the achilles-tendon-type suspension is centrifuged, a salt solution is discarded, residues are washed through water, centrifuging is conducted, and the smashed achilles-tendon-type powder is obtained. The smashing method provided by the invention is simple and easy to implement, energy and electricity are saved, the production efficiency is high, and the smashing method is suitable for industrialized production.

Description

technical field [0001] The invention relates to the technical fields of biotechnology, medical equipment and daily chemical industry, in particular to a method for pulverizing animal tendon and its application. Background technique [0002] Collagen is widely present in animal connective tissues such as tendons, ligaments, cartilage, skin, teeth, etc., plays a supporting role in organs, endows tissues with mechanical strength, and plays an important role in cell differentiation, motility tendency, and repair of connective tissues. Collagen is widely used in biomedical materials and clinical medicine due to its natural hemostasis, low toxicity, low antigenicity, low immunity, ability to guide cell regeneration, and good compatibility with the human body. [0003] Because tendon tissue has strong toughness, it is difficult to slice and crush without special treatment, which directly affects the extraction efficiency of collagen. Because the Achilles tendon is derived from ani...

Claims

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Application Information

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IPC IPC(8): B02C21/00C07K14/78C07K1/34
CPCB02C21/00C07K1/34C07K14/78
Inventor 彭新生张智武陈新亮吴文静许新佳周艳芳崔碧玲
Owner 浙江崇山生物制品有限公司
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