Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

An analytical method for rapid detection of metabolic markers in smoke

A technology of metabolic markers and analysis methods, which is applied in the field of rapid detection of metabolic markers in smoke gas, to achieve high-sensitivity detection, high-throughput analysis, prevention of self-degradation, and simple operation

Active Publication Date: 2020-05-05
ZHENGZHOU TOBACCO RES INST OF CNTC +1
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there has been no report on the analysis of metabolites in flue gas by nanoliter liquid chromatography tandem mass spectrometry.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An analytical method for rapid detection of metabolic markers in smoke
  • An analytical method for rapid detection of metabolic markers in smoke
  • An analytical method for rapid detection of metabolic markers in smoke

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0035] The preparation of specific embodiment 1 β-glucuronidase reactor

[0036](1) Take 300μL GMA and 100μL TRIM in a 1.5mL centrifuge tube, vortex for 5min;

[0037] (2) Take 850 μL cyclohexanol and 150 μL dodecanol in a 1.5 mL centrifuge tube and vortex for 5 min;

[0038] (3) Mix 250 μL of GMA-TRIM solution with 750 μL of cyclohexanol-dodecanol solution;

[0039] (4) Add 0.0100g of AIBN to dissolve in the above solution, ultrasonically degas for 20 minutes, vortex for 10 minutes to a clear and transparent solution, pour into the activated capillary column, seal both ends, and polymerize in a constant temperature water bath at 50°C 24h; then wash with methanol;

[0040] (5) Rinse the prepared porous organic polymer monolithic column with 18.25 MΩ ultrapure water for 30 min, and the pH value is neutral as detected by pH test paper; rinse the porous organic polymer monolithic column with 5 mM ammonium acetate buffer solution;

[0041] (6) Dispense 20 μL of 2.18 mg.mL with ...

Embodiment 2

[0043] Example 2 Collection and pretreatment of enzymatic hydrolyzate

[0044] Take 10 μL concentration is 0.05mg.mL -1 , 0.1mg.mL -1 , 0.2mg.mL -1 , 0.5mg.mL -1 , 0.8mg.mL -1 , 1.0mg.mL -1 The substrate NNAL-O-Glu passed through the organic polymer monolithic column enzyme reactor at a flow rate of 0.6 μL / min, and the enzymatic solution was collected in a 1.5mL centrifuge tube. At the same time, free hydrolyzate with concentration of 0.05mg.mL-1, 0.1mg.mL-1, 0.2mg.mL-1, 0.5mg.mL-1, 0.8mg.mL-1, 1.0mg.mL-1 was prepared in 1.5mL centrifuge tube and place it in a 37°C water bath. After 24 hours, remove the free hydrolyzate in the water bath.

[0045] The enzymatic solution was desalted, concentrated, reconstituted in 1 mL of 0.1% formic acid-water, sonicated for 15 minutes, and centrifuged at 13300 rpm / min 3 times, 10 minutes each time. Vortex at 3000rmp / min for 5 minutes, pipette the supernatant, and filter with a 0.22 μm organic phase needle filter; perform Nano ESI-RPL...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an analysis method for rapidly detecting an in-vivo metabolism marker of smoke. The analysis method comprises a smoke sample purification-enrichment pretreatment step and an analysis detection step. A metabolite is cotinine or 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL). The analysis method is characterized in that the metabolite is firstly hydrolyzed by virtue ofa beta-glucuronidase reactor and then is analyzed and detected by virtue of a nano-flow liquid chromatography-mass spectrometry. The innovation points of the invention are as follows: by preparing online through an enzyme reactor, the hydrolysis effect is relatively good, the experimental steps can be simplified, and the analysis speed and the analysis sensitivity can be increased; a nano-flow liquid chromatography tandem mass spectrometry is applied to the field of smoke metabolites for the first time; and the sample amount required by an experiment is extremely low, only micro-nanogram-scale samples are required under a nano-flow condition, and high-sensitivity detection and high-throughput analysis can be realized.

Description

technical field [0001] The invention relates to a detection method for metabolic markers in smoke gas, which belongs to the technical field of analysis and detection, in particular to an analysis method for rapidly detecting metabolic markers in smoke gas, and the metabolite is 4-(methylnitrosamine) - 1-(3-pyridyl)-1-butanol (NNAL). Background technique [0002] 4-(Methylnitrosamine)-1-(3-pyridyl)-1-butanone (NNK) is a tobacco-specific N-nitrosamine (TSNAs) with high carcinogenic activity, Research Institute (IARC) listed as a carcinogen. It is a smoke carcinogen formed by the nitrosylation of nicotine or a small amount of nicotine nitrosylation, and its metabolite 4-(methyl nitroso Amino)-1-(3-pyridyl)-1-butanol (NNAL) and its glycosidic derivative NNAL-Glucs are very valuable biomarkers for studying the mechanism of toxicity and detoxification of NNK in the metabolism of human body things. 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol is the It is the main metabolite ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 邓楠郭益张明建张柯王乐李斌张书胜
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com