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Application of a strain of Candida in the prevention and control of postharvest fruit diseases

A technology for fruit postharvest diseases and Candida, which is applied in the fields of application, fruit and vegetable preservation, and microbial-based methods, can solve the problems that the biocontrol effect is only verified on a small number of fruits, and there is a lack of antibacterial spectrum strains, etc. Achieve remarkable social and ecological benefits, stable properties, and wide antibacterial spectrum

Active Publication Date: 2021-03-02
北京华康信使科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, although there are nearly a hundred species of antagonistic yeasts reported at home and abroad, the biocontrol effects of most antagonistic yeasts have only been verified on a few fruits.
However, since the biocontrol effects of different strains of the same yeast are very different (Filonow et al., 1996), most of the antagonistic yeasts lack strains with broad antibacterial spectrum and stable effect.

Method used

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  • Application of a strain of Candida in the prevention and control of postharvest fruit diseases
  • Application of a strain of Candida in the prevention and control of postharvest fruit diseases
  • Application of a strain of Candida in the prevention and control of postharvest fruit diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Biological properties of Candida anatomiae BY13 strain

[0025] 1. Morphological characteristics

[0026] (1) YPDA medium (1% yeast extract powder, 2% peptone, 2% glucose, 1.8% agar, sterilized at 121°C for 20 minutes) was cultured at 26°C for 48h, and the colonies were round and white with smooth and round edges. The cell shape is ellipsoidal.

[0027] (2) After culturing in YPDA liquid medium for 24 hours, no mold was formed, the bacterial solution was turbid, and there was precipitation. Microscopically, the yeast cells were oval and budded.

[0028] 2. Molecular biological identification

[0029] Use the universal forward primer NL-1 (5'-GCATATCAATAAGCGGAGGAAAAG-3') and the reverse primer NL-4 (5'-GGTCCGTGTTTCAAGACGG-3') to PCR amplify the yeast 26S rDNA D1 / D2 region nucleic acid sequence, and PCR The sequencing results of the product were entered into the website www.NCBI.nlm.nih.gov for BLAST, the homologous sequences were downloaded from the GenBank...

Embodiment 2

[0031] Example 2 Inhibition of Candida BY13 to Apple Penicillium and Botrytis Botrytis

[0032] 1. Experimental protocol

[0033] Candida BY13 was taken out from the -80°C refrigerator, activated with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and picked a single Colony into YPD liquid medium, culture at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on a hemocytometer to prepare the concentration 1×10 8 cells / mL of Candida BY13 suspension.

[0034]Activate Penicillium expansum or Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Penicillium or Botrytis cinerea spore suspension.

[0035] Healthy and undamaged apple fruits ...

Embodiment 3

[0042] Example 3 Inhibitory effect of Candida BY13 on pear fruit blue mold and gray mold

[0043] 1. Experimental protocol

[0044] Candida BY13 was taken out from the -80°C refrigerator, activated with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and picked a single Colony into YPD liquid medium, culture at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on a hemocytometer to prepare the concentration 1×10 8 cells / mL of Candida BY13 suspension.

[0045] Activate Penicillium expansum or Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Penicillium or Botrytis cinerea spore suspension.

[0046] Disinfect healthy and undamaged ...

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Abstract

The invention discloses Candida anatomiae strain BY13 with wide antibacterial spectrum and stable effect for preventing and controlling postharvest diseases of fruits and vegetables and a use method thereof. The preservation strain number of the strain in the General Microorganism Center of China Microbiological Culture Collection Management Committee is CGMCC No.14901. The method of using the Candida: activate the strain, ferment and cultivate it with YPD, centrifuge, and use sterile water to make 1×10 8 cells / mL bacterial suspension; put apples, pears, grapes, strawberries, citrus or cherry tomatoes and other fruits and vegetables into the bacterial suspension, soak for 30 seconds, take out, and air-dry; put them in a fresh-keeping box and store at room temperature. This Candida strain simultaneously controls Apple Penicillium and Botrytis Botrytis, Pear Penicillium and Botrytis Botrytis, Botrytis Botrytis, Anthracnose, Aspergillosis, Black Spot and Fusarium, Strawberry Botrytis Aspergillosis and aspergillosis, citrus penicillium, and cherry tomato gray mold and aspergillosis, reducing losses caused by postharvest diseases, has a good application prospect.

Description

technical field [0001] The invention relates to the field of biological control of postharvest diseases of fruits, in particular to the application of a strain of Candida anatomiae in the prevention and control of postharvest diseases of fruits. All diseases have significant control effect. Background technique [0002] The quality deterioration of fresh fruit products is affected by many factors, but disease is the main reason. Among them, rot and deterioration caused by fungal diseases is the most serious factor in postharvest fruit loss. Although postharvest diseases of fruits can be controlled through many methods such as agricultural control, physical control, chemical control and biological control, the main measure to control postharvest diseases of fruits is chemical control (Eckert & Ogawa, 1985, 1988). Today, people are paying more and more attention to pollution-free food. While fresh fruit occupies an increasing amount in food, its safety has also been closely ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A23B7/155C12R1/72
CPCA23B7/155C12N1/16C12N1/165C12R2001/72
Inventor 王友升陈滢姚婷黄津津马琳
Owner 北京华康信使科技有限公司
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