Bacterium D10 for improving validity of heavy metal and application of bacterium D10

A heavy metal, effective technology, applied in the fields of agricultural technology and environmental pollution control, can solve the problem of small biomass, achieve the effect of promoting plant growth and increasing the content of heavy metals in plants

Active Publication Date: 2018-04-20
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biomass of herbaceous alfalfa is small, so

Method used

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  • Bacterium D10 for improving validity of heavy metal and application of bacterium D10
  • Bacterium D10 for improving validity of heavy metal and application of bacterium D10
  • Bacterium D10 for improving validity of heavy metal and application of bacterium D10

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The heavy metal-resistant plant growth-promoting bacteria D10 strain of the present invention is isolated and purified from alfalfa (Medicogo sativa L.) root nodules grown in heavy metal-contaminated soil, and the isolation and identification method is as follows:

[0039] Gently pull the alfalfa out of the soil, shake off the loose soil from the roots and rinse with water. Select the plump and large-diameter pink nodules on the main root, and cut them off with scissors. Soak the cut root nodules in deionized water for 4-5 minutes to remove residual impurities, soak them in 75% ethanol for 2-3 minutes, wash them with water for 2-3 times, soak them in 3% NaClO solution for 5 minutes, and finally wash them with sterile deionized water Wash with water 5-7 times. Under aseptic conditions, put the surface-sterilized nodules on a YMA solid plate containing 0.3% Congo red [mannitol 10.0g, yeast powder 1.0g, MgSO 4 ·7H 2 O 0.2g, K 2 HPO 4 0.5g, NaCl 0.1g, anhydrous CaCl ...

Embodiment 2

[0041] Activation of embodiment 2 bacterial strain D10 and bacterial suspension preparation

[0042] Inoculate D10 (CCTCC NO: M 2016554) slant bacteria into TY solid medium (tryptone 5.0g, yeast extract 3.0g, CaCl 2 0.3g, 20g agar, 1000ml distilled water, pH 7.0), cultured at 30°C for 3d. Then select plump, viscous D10 colonies and inoculate them in TY liquid medium (tryptone 5.0g, yeast extract 3.0g, CaCl 2 0.3g, distilled water 1000ml, pH7.0), 30°C 150rpm shaking culture for 20-24h, so that the number of cells can reach more than 500 million CFU / ml.

Embodiment 3

[0043] The ability of embodiment 3 bacterial strain D10 to produce IAA

[0044] According to the determination method of Gordon and Weber (1951), TY liquid medium was divided into test tubes, 4 mL per tube, and 1 mL of 2.5 mg / mL tryptophan solution sterilized by filtration was added after high-temperature sterilization at 121 ° C to make the color in the medium The final concentration of amino acid was 0.5 mg / mL. D10 (CCTCC NO: M 2016554) was inoculated in the above medium, and cultured in a shaker at 30°C for 3 days. The fermentation broth was centrifuged at 12000r / min for 5min, 1mL of the supernatant was taken, 50μL of 10mM orthophosphoric acid was added, and 2mL of Sackowski's chromogenic reagent was added, mixed thoroughly, the color was developed at 25°C for 30min in the dark, and the absorbance value was measured at a wavelength of 530nm. The sterile medium was treated the same as above and set to zero as a control. Use the IAA standard solutions with concentrations of...

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Abstract

The invention discloses bacterium D10 for improving the validity of heavy metal and an application of the bacterium D10. The bacterium D10 is named as Sinorhizobium meliloti D10 according to classification and is preserved at the China Center for Type Culture Collection with the culture preservation number being CCTCC NO: M 2016554. A D10 bacterial strain is taken as a raw material and is activated by a culture medium to prepare a bacterium suspending liquid. Soil polluted by heavy metal in a mining area is inoculated with the D10 bacterium suspending liquid, which can promote growth of energyplants, increases the extractable copper content of rhizosphere soil of plants, and improves the copper content and total copper absorptive amount of plant roots. Alfalfa and rye grass that are inoculated with D10 can fix and restore soil polluted by heavy metal, and sweet sorghum inoculated with D10 can perform fixed restoration and extraction restoration. After inoculation with the D10 bacterium suspending liquid, the content of IAA produced by rhizosphere microorganisms of plants is increased, and the fertility of soil is improved.

Description

technical field [0001] The invention belongs to the application fields of agricultural technology and environmental pollution control, and relates to a bacterium D10 for improving the effectiveness of heavy metals and an application thereof. Background technique [0002] In recent years, heavy metal pollution in farmland due to mining activities has had a serious impact on food safety and human health. Phytoremediation is an environmentally friendly control measure that can control heavy metal pollution. However, most hyperaccumulators are difficult to grow in the wild due to their harsh agricultural production requirements, and their biological yield is relatively small, which limits the large-scale practical application of hyperaccumulators in farmland. It is feasible to use energy plants (such as sweet sorghum) with high biomass, fast growth and strong tolerance to heavy metals as plant extraction materials. [0003] Rhizobia can not only fix nitrogen symbiotically with...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10A01G7/06A01B79/02C12R1/01
CPCA01B79/02A01G7/06B09C1/10C12N1/205C12R2001/01
Inventor 何琳燕盛下放王琪严警
Owner NANJING AGRICULTURAL UNIVERSITY
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