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A kind of technology of cultivating spirulina platensis

A technology of Spirulina platensis and process, applied in the direction of bacteria, etc., can solve the problems of slow growth of algae cells, low yield of polysaccharides, and high cost of cultivation of Spirulina platensis, so as to improve corporate profits, increase leaching rate, and promote algae cell growth. proliferating effect

Active Publication Date: 2021-01-29
鄂尔多斯市蒙健螺旋藻业有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the defects of the prior art such as high cost of cultivation of Spirulina platensis, slow growth of algae cells and low yield of polysaccharides, and provides a compound medium for cultivating Spirulina platensis

Method used

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  • A kind of technology of cultivating spirulina platensis
  • A kind of technology of cultivating spirulina platensis
  • A kind of technology of cultivating spirulina platensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of technique of cultivating spirulina platensis, it comprises the steps:

[0030] Step 1) Take 0.6g of sodium nitrate, 0.3g of sodium bicarbonate, 0.1g of sodium chloride, 0.03g of ferric ammonium citrate, 0.02g of sodium molybdate, 0.01g of zinc sulfate, 0.001g of propionamide, 20ml of wheat bran extract, Add 20ml of rice straw extract into sterile water, adjust the pH to 8, and set the volume to 1L to prepare a culture medium for Spirulina platensis;

[0031] Step 2) Take Spirulina platensis in the logarithmic growth phase, inoculate it into the medium of Spirulina platensis according to the inoculum amount of 5% for cultivation, control the light intensity at 3500lux, control the temperature at 32°C, and the air flow rate of 0.4vvm, cultivated for 8 days.

[0032] The wheat bran extract is prepared according to the following process:

[0033] Grind the wheat bran and place it in a reaction tank, add 2 times the weight of 6M hydrochloric acid, stir and hydrol...

Embodiment 2

[0037] A kind of technique of cultivating spirulina platensis, it comprises the steps:

[0038] Step 1) Take 0.4g of sodium nitrate, 0.4g of sodium bicarbonate, 0.1g of sodium chloride, 0.04g of ferric ammonium citrate, 0.02g of sodium molybdate, 0.01g of zinc sulfate, 0.001g of propionamide, and 15ml of wheat bran extract, Add 25ml of rice straw extract to sterile water, adjust the pH to 8, and set the volume to 1L to prepare a culture medium for Spirulina platensis;

[0039]Step 2) Take Spirulina platensis in the logarithmic growth phase, inoculate it into the medium of Spirulina platensis according to the inoculation amount of 7% for cultivation, control the light intensity at 3500lux, control the temperature at 33°C, and the air flow rate of 0.3vvm, cultivated for 7 days.

[0040] The wheat bran extract is prepared according to the following process:

[0041] Grind the wheat bran and place it in a reaction tank, add 2 times the weight of 6M hydrochloric acid, stir and hy...

Embodiment 3

[0045] The present invention cultivates the effect test of Spirulina platensis technique:

[0046] The experimental group is Example 1; the control group 1 uses Zaruk culture medium; the control group 2 does not add wheat bran extract, and the rest is the same as in Example 1; the control group 3 does not add rice straw extract, and the rest is the same as in Example 1 ; The remaining culture conditions are the same as above, and the experimental group and the control group 1-3 are the same. The cell density (OD560) of Spirulina platensis in the medium was detected by a spectrophotometer every other day, and the density on day 0 was the density at the time of inoculation; see Table 1 for details:

[0047] Table 1

[0048] number of days test group Control group 1 Control group 2 Control group 3 0 0.26 0.26 0.26 0.26 2 0.69 0.43 0.51 0.59 4 1.53 0.98 0.99 1.21 6 2.51 1.77 1.81 2.26 8 3.14 2.20 2.33 2.62

[0049] Co...

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Abstract

The invention belongs to the technical field of algae cultivation, and discloses a process for cultivating spirulina platensis, which comprises the following steps: taking 0.4-0.6g of sodium nitrate, 0.3-0.5g of sodium bicarbonate, 0.1-0.2g of sodium chloride, lemon Ammonium ferric acid 0.03‑0.05g, sodium molybdate 0.01‑0.02g, zinc sulfate 0.01‑0.02g, propionamide 0.001‑0.002g, wheat bran extract 10‑20ml, rice straw extract 20‑30ml, added to sterile In water, adjust the pH to 8, and set the volume to 1L to prepare a Spirulina platensis medium; take the Spirulina platensis in the logarithmic growth phase, and inoculate the above-mentioned Spirulina platensis medium according to the inoculum size of 5-7%. Cultured in medium for 6-8 days. The process of the invention has low cost, is simple and feasible, has fast algae growth speed and high polysaccharide yield.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, in particular to a process for cultivating spirulina platensis. Background technique [0002] Spirulina is an unbranched filament composed of a single row of cells. The colloidal sheath has no or only a very thin sheath, and has a regular spiral shape to form algae. No heterocyst and posterior wall spores. About 38 species of Spirulina have been found so far, most of which grow in alkaline salt lakes. At present, there are large-scale artificial cultivation at home and abroad, mainly three kinds of Spirulina platensis, Spirulina maxima and Spirulina indica. Edible, nutritious, protein content as high as 60%-70%. In natural waters, its massive reproduction will form algal blooms. The optimal growth pH range of Spirulina is 3-11.0, and when the pH is higher than 11.0, it will be unfavorable for growth. Under the condition of normal nutrition and temperature, light becomes an importan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 吕兰婷吕伟红陈金华陈国汀陈国华黄土根
Owner 鄂尔多斯市蒙健螺旋藻业有限责任公司
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