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Bacillus amyloliquefaciens strain, lipopeptid mixture produced by bacillus amyloliquefaciens strain, and relevant application

A technology for dissolving starch spores and mixtures, applied in the application, microorganism, microorganism and other directions, can solve the problems of unstable control effect in the field, short control time, less practical application, etc., and achieve good inhibitory effect, wide control effect and strong inhibitory effect. Effect

Active Publication Date: 2018-05-08
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the Bacillus biocontrol preparations are currently in the experimental stage, with little practical application, and there are problems such as unstable control effect in the field and short control time.

Method used

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  • Bacillus amyloliquefaciens strain, lipopeptid mixture produced by bacillus amyloliquefaciens strain, and relevant application
  • Bacillus amyloliquefaciens strain, lipopeptid mixture produced by bacillus amyloliquefaciens strain, and relevant application
  • Bacillus amyloliquefaciens strain, lipopeptid mixture produced by bacillus amyloliquefaciens strain, and relevant application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Screening of Bacillus amyloliquefaciens bacterial strain

[0026] 1. Isolation of strains

[0027] (1) Take 10g of soil samples from Mount Huanggang, Wuyishan City, Fujian Province in 90mL sterile water, and after fully shaking, draw 1mL of soil suspension for gradient dilution;

[0028] (2) apply the soil dilution obtained in step (1) on the NA solid medium plate, then place the plate in a 30°C constant temperature incubator and cultivate it for 48h;

[0029] Among them, the components of the NA solid medium are: peptone 0.5%, beef extract 0.3%, glucose 1.0%, agar 1.8%, prepared with distilled water, pH 7.2, and the percentages in each component are weight ratios.

[0030] (3) First pick the single colony obtained in step (2) to carry out smear, microscopic examination after basic fuchsin staining, select the bacterial strain capable of producing spores and transfer it to the slant medium for preservation as the bacterial strain to be tested, and then pu...

Embodiment 2

[0046] Example 2: Preparation and identification of lipopeptide mixture

[0047] 1. Preparation of lipopeptide mixture

[0048] (1) Activation of Bacillus amyloliquefaciens strain FJAT-46737: Streak the Bacillus amyloliquefaciens strain FJAT-46737 on the NA solid medium of the strain with an inoculation loop, and cultivate it in a constant temperature incubator for 48 hours, and set the culture temperature to Set at 30°C; the components of the NA solid medium are: beef extract 0.3%, peptone 0.5%, glucose 1.0%, agar 1.8%, prepared with distilled water, pH 7.0-7.2, the percentage of each medium component Both are weight ratios.

[0049] (2) Preparation of seed solution: the single bacterium colony of Bacillus amyloliquefaciens FJAT-46737 obtained in step (1) is inoculated in the Erlenmeyer flask (bottle capacity is 50mL / 250mL Erlenmeyer flask) that is filled with seed medium, and The triangular flask was placed in a constant temperature shaker for shaking culture, the rotation...

Embodiment 3

[0081] Embodiment 3: Pot control test of lipopeptide mixture to tomato bacterial wilt

[0082] (1) Test material

[0083] The tested variety: Nongke 180 tomato, purchased from Fujian Nongke Agricultural Variety Development Co., Ltd.

[0084] (2) Test method

[0085] The test site was set in the greenhouse of Fujian Academy of Agricultural Sciences, and treatment group 1 (the concentration of Bacillus was 1.0×10 8 CFU / mL), treatment group 2 (2-fold dilution treatment group of fermentation supernatant), treatment group 3 (1mg / ml lipopeptide mixture soaking roots for 1h), positive control group (only inoculated with R. solanacearum), negative For the control group (clear water), 30 tomato seedlings were tested in each group, 3 plants in each pot.

[0086] Treatment group 1: Bacillus FJAT-46737 was activated on NA solid medium plate, inoculated in PDB liquid medium, cultured at 170rpm, 25°C for 48h, and diluted to 10 8 CFU / mL;

[0087] Treatment group 2: After centrifuging th...

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Abstract

The invention provides a bacillus amyloliquefaciens strain, a lipopeptid mixture produced by bacillus amyloliquefaciens strain, and relevant application, and belongs to the technical field of microbial fermentation. The strain is bacillus amyloliquefaciens FJAT-46737 and is conserved in China Microbe Preservation Management Committee General Microbiological Culture Center on September 22, 2017, and the preservation serial number is CGMCC No.14661. The ipopeptid mixture produced by the bacillus amyloliquefaciens strain comprises C 14-C 16 iturin A, C 14-C 16 surfactin, C 16 fengycin A, C 16 fengycin A 2, C 16 fengycin B and C 16 fengycin B 2. The lipopeptid mixture can well prevent and control pathogenic bacteria of plants and animals, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and specifically relates to a strain of Bacillus amyloliquefaciens, a lipopeptide mixture produced by fermentation of the strain, and its application in preventing and treating pathogenic bacteria. Background technique [0002] Tomato bacterial wilt is a devastating soil-borne vascular disease caused by Ralstonia solanacearum, which can reduce yield by 10% to 30% in mild cases, or reduce yield by more than 50% or even fail in severe cases. Huge economic loss. The pathogen is mainly transmitted through rainwater, irrigation water and agricultural tools. The pathogen invades from the wound of the host root or stem base, and expands in the vascular tissue of the plant, causing duct blockage and cell poisoning. At present, the control of tomato bacterial wilt mainly adopts cultivation measures, crop rotation, variety resistance selection, chemical agents, etc., but with little effect....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P21/00A01N63/02A01P1/00A01P3/00C12R1/07
CPCA01N63/10C12P21/00C12N1/205C12R2001/07
Inventor 朱育菁陈梅春刘波王阶平陈峥杨文靖
Owner 福建省农业科学院农业生物资源研究所
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