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Separation fraction e of ethyl acetate extract of Sargassum henzii and its preparation method and application in marine fouling biological control

A technology of ethyl acetate extraction and Sargassum Heinz, which is applied in the biological control of marine fouling, the separation component E of Sargassum Heinz ethyl acetate extract and the field of preparation thereof, achieving wide sources, great promotion potential, separation The effect of easy preparation

Active Publication Date: 2020-05-19
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report on whether Sargassum henzii contains active ingredients against larvae attachment and spore germination of marine fouling organisms and whether it can be applied to the field of marine fouling biocontrol

Method used

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  • Separation fraction e of ethyl acetate extract of Sargassum henzii and its preparation method and application in marine fouling biological control
  • Separation fraction e of ethyl acetate extract of Sargassum henzii and its preparation method and application in marine fouling biological control

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The Separated Component E of the Sargasso Heinzia ethyl acetate extract is obtained from Sargasso Heinzia. The separation and extraction steps include:

[0023] (1) Extract Sargassum henslowianum C.Agardh with a volume fraction of 95% ethanol aqueous solution for three times, combine the extracts to obtain an ethanol extract, and concentrate by rotary evaporation at 45°C to remove the ethanol to obtain a paste extract;

[0024] (2) The paste-like extract was resuspended in water at the ratio of 6 ml per gram, and extracted with an equal volume of petroleum ether three times, discarded the petroleum ether extraction components, and the remaining aqueous layer was extracted three times with an equal volume of ethyl acetate. , The combined ethyl acetate extracts are concentrated and dried to obtain ethyl acetate extracts;

[0025] (3) The ethyl acetate extract was chromatographed on a normal pressure silica gel column (silica gel 8000g, column length 180cm, column diameter 20cm),...

Embodiment 2

[0028] Experimental group: Dissolve component E of the ethyl acetate extract of Sargassum heinii in a mixed solvent of chloroform and methanol (the mixed solvent of chloroform and methanol is a mixture of chloroform and methanol in a volume ratio of 3:1) to prepare a concentration of 282.6μg / ml The solution. Add 1 ml of this solution to a petri dish with a diameter of 6 cm and make it evenly cover the bottom of the petri dish. After the solvent is completely volatilized, the dose of the separated component E of the Sargassum heinensis ethyl acetate extract coated on the bottom of the petri dish is 10 μg / cm 2 . Then add 13ml of sea water.

[0029] Control group: Add 1ml of chloroform-methanol mixed solvent (chloroform-methanol mixed solvent is a mixture of chloroform and methanol in a volume ratio of 3:1), make it evenly distributed on the bottom of the petri dish, and add 13ml of sea water after the volatilization is complete.

[0030] Blank group: 13ml of seawater was added to t...

Embodiment 3

[0036] Experimental group: Dissolve component E of the ethyl acetate extract of Sargassum heinii in a mixed solvent of chloroform and methanol (the mixed solvent of chloroform and methanol is a mixture of chloroform and methanol in a volume ratio of 3:1) to prepare a concentration of 282.6μg / ml The solution. Add 1 ml of this solution to a petri dish with a diameter of 6 cm and make it evenly cover the bottom of the petri dish. After the solvent is completely volatilized, the ethyl acetate extract of Sargassum heinzii is coated on the bottom of the petri dish. The dose of the separated component E is 10μg / cm 2 . Then add 13ml of sea water.

[0037] Control group: Add 1ml of chloroform-methanol mixed solvent (chloroform-methanol mixed solvent is a mixture of chloroform and methanol at a volume ratio of 3:1), distribute the solvent evenly on the bottom of the petri dish, and add 13ml of sea water after the solvent is completely evaporated.

[0038] Blank group: 13ml of seawater was ...

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Abstract

The invention discloses a sargassum henslouianum ethyl acetate extract separating component E, a preparation method and application thereof in marine fouling organism control. When low dosage of sargassum henslouianum ethyl acetate extract separating component E is covered on a solid surface, the sargassum henslouianum ethyl acetate extract separating component E has a remarkable effect on inhibiting barnacle and mussel larvae settlement so as to be used for preparing a marine fouling organism control agent. The sargassum henslouianum ethyl acetate extract separating component E is extracted from sargassum henslouianum, is a natural organic compound, cannot pollute a water environment, cannot be enriched in organism caused by transferring through a food chain, is environmentally-friendly and high in safety, can effectively inhibit marine organism settlement, does not contain heavy metal elements such as copper and tin at the same time, has a favorable social benefit from the perspective of environmental protection, is wide in sources, simple and convenient to separate and prepare, suitable for mass production, and large in popularization potential, and has a favorable application prospect in marine fouling organism control.

Description

Technical field: [0001] The invention belongs to the field of natural products, and in particular relates to the separation component E of the Sargassum heinii ethyl acetate extract, a preparation method thereof, and application in the control of marine fouling organisms. Background technique: [0002] Marine fouling organisms refer to all kinds of organisms that are fixed or inhabit the underwater parts of ships and artificial facilities and have an adverse impact on human economic activities. The main hazards are increased resistance, reduced speed, and increased fuel consumption; blocked pipeline systems and changed The metal corrosion process causes corrosion perforation; increases the dynamic load effect, causing man-made facilities to drift, unbalance and even overturn; compete with the breeding objects for attachment base and bait, hinder their growth and development, and reduce the quality of aquatic products. Therefore, the prevention and control of marine fouling organi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/03A01P17/00
CPCA01N65/03
Inventor 曹文浩吴慧严涛卢虹玉林明晴章超桦韩帅帅
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI