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A method for preparing zno nanoparticles using recombinant collagen as biomineralization template

A technology of recombining collagen and biomineralization, applied in chemical instruments and methods, nanotechnology, nanotechnology, etc., can solve the problems of lower light transmittance of water body and damage to ecological environment

Active Publication Date: 2020-10-27
兰州生物技术开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Organic dyes are the main components of printing and dyeing wastewater, such as the representative cationic dye rhodamine B, which has strong fluorescent properties in solution, even if the concentration is very low, it will reduce the light transmittance of the water body and seriously damage the ecological environment

Method used

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  • A method for preparing zno nanoparticles using recombinant collagen as biomineralization template
  • A method for preparing zno nanoparticles using recombinant collagen as biomineralization template
  • A method for preparing zno nanoparticles using recombinant collagen as biomineralization template

Examples

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Embodiment 1

[0037] Example 1 A method for preparing ZnO nanoparticles using recombinant collagen as a biomineralization template

[0038] A method for preparing ZnO nanoparticles with collagen as biomineralization template, comprising the steps of:

[0039] (1) Preparation of recombinant collagen by biogenetic engineering technology

[0040] ① Determine the sequence of recombinant collagen:

[0041] The sequence of recombinant collagen is:

[0042] GSPGLPGPRGEQGPTGPTGPAGPRGLQGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGRPGKRGKQGQKGEKGEPGTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGFPGERGVEGQNGQDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPGKYGPPGPPGPPGPPGPPGPPGPPGPPGPPGPP,该重组胶原蛋白具备良好的三重螺旋结构,热变温度接近37℃;

[0043] ② Synthesis of nucleic acid encoding recombinant collagen:

[0044] Synthesis of coding steps ① recombining the nucleic acid of collagen, constructing a plasmid for introducing the above nucleic acid, and transforming the plasmid into Es...

Embodiment 2

[0060] Example 2 An experiment on the photocatalytic degradation of organic dyes prepared by using recombinant collagen as a biomineralization template to prepare ZnO nanoparticles

[0061] 1) Preparation of a homogeneous mixed solution of zinc oxide nanoparticles and organic dyes;

[0062] Prepare an organic dye solution of 4-100 mg / L, add 2-10 mg of zinc oxide nanoparticles prepared in Example 1, and react in the dark for 10-120 min, so that the solution reaches absorption and resolution equilibrium;

[0063] 2) Zinc oxide nanoparticles degrade organic dyes under light conditions;

[0064] Irradiate the mixed solution with ultraviolet light for 0.5-48hrs;

[0065] 3) UV spectrophotometer tracks the degradation level of organic dyes in different time periods;

[0066] Use UV-1750 ultraviolet spectrophotometer to analyze the ultraviolet absorption of organic dyes at different irradiation times;

[0067] Such as Figure 6 (Effect diagram of nano-ZnO on the catalytic degrada...

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Abstract

The invention discloses a method for preparing ZnO nanoparticles with recombinant collagen as a biomineralization template, which specifically comprises the following steps: (1) preparation of recombinant collagen by utilizing biological genetic engineering technology: (a) determining the sequence of recombinant collagen; (b) synthesizing and coding the nucleic acid of the recombinant collagen; (c) preparing and purifying recombinant collagen; (2) preparation of ZnO nanomaterial: (a) preparing a uniformly mixed solution of the recombinant collagen, zinc nitrate hexahydrate and sodium hydroxide; (b) putting the mixed solution into a 25 DEG C incubator; (c) purifying, drying and storing the prepared nanomaterial. The method adopts the recombinant collagen as a biological template and zinc nitrate hexahydrate as a material to prepare the size-and-morphology-controllable ZnO nanomaterial under room temperature. The method is simple and convenient, and is easy to operate, the prepared ZnO nanomaterial shows excellent degradation capability on a variety of organic dyes, and has a tremendous application prospect in the treatment of dye wastewater.

Description

technical field [0001] The invention belongs to the technical field of preparation of biological inorganic materials, and in particular relates to a method for preparing ZnO nanoparticles by using recombinant collagen as a biomineralization template. Background technique [0002] With the rapid development of the global economy, people begin to pay attention to the protection of the environment, and pay special attention to the problem of water pollution. my country's printing and dyeing wastewater has the characteristics of large discharge, many refractory substances, complex organic components, and deep color. It is urgent to develop efficient treatment methods. Organic dyes are the main components of printing and dyeing wastewater, such as the representative cationic dye Rhodamine B, which has strong fluorescence in solution, even if the concentration is very low, it will reduce the light transmittance of the water body and seriously damage the ecological environment. At...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01G9/02B82Y30/00C07K14/78C12N15/70
CPCB82Y30/00C01G9/02C01P2002/72C01P2002/85C01P2002/88C01P2004/03C01P2004/04C07K14/78C12N15/70
Inventor 肖建喜何会霞
Owner 兰州生物技术开发有限公司