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Cassava raw material saccharification method and novel process for producing ethanol by method

A new process, cassava technology, applied in the direction of biofuel, fermentation, etc., can solve the problems of low alcohol yield and raw material utilization rate, high energy consumption of alcohol fermentation production, etc., to improve raw material utilization rate and alcohol yield, and promote yeast The effects of cell reproduction and shortening the fermentation cycle

Inactive Publication Date: 2018-05-22
罗绍庆
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the present situation in the prior art that the alcohol fermentation production consumes a lot of energy, and the alcohol yield and raw material utilization rate are low, the present invention provides a cassava raw meal saccharification method and a new process for producing alcohol by using the method

Method used

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  • Cassava raw material saccharification method and novel process for producing ethanol by method
  • Cassava raw material saccharification method and novel process for producing ethanol by method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A. Crush the disease-free dry cassava slices and cross 40 meshes to obtain cassava flour, then mix the cassava flour with 0.4g / L yeast extract, 0.10g / L urea, 1.4g / L peptone, and 20mg / L calcium sulfate. Mix in 60℃ warm water and prepare 25% (W / V) tapioca powder solution;

[0047] B. Add 5 times the amount of loaded ionic liquid biochar to the tapioca powder solution for reaction for 2h, then add reverse micelle-enzyme, hydrolyze the raw meal at 45℃ for 30min, centrifuge at 2000r / min, and take the upper phase solution , Get starch paste;

[0048] The reverse micelle-enzyme composition is: 214IU / g cassava flour alpha-amylase and 398IU / g cassava flour glucoamylase;

[0049] The preparation method of the loaded ionic liquid biochar in step B is as follows:

[0050] (1) The biochar with a particle size of 250μm was reacted with ethanol solution, 1mol / L hydrochloric acid solution, and deionized water at a solid-to-liquid ratio of 1:2 at 20°C for 4 hours with magnetic stirring, filter...

Embodiment 2

[0058] A. Crush the disease-free dry cassava flakes and cross 100 meshes to obtain cassava flour, then mix the cassava flour with 0.6g / L yeast extract, 0.14g / L urea, 1.6g / L peptone, and 30mg / L calcium sulfate. Mix in warm water at 80°C and prepare a 35% (W / V) tapioca solution;

[0059] B. Add 10 times the amount of loaded ionic liquid biochar to the tapioca powder solution for 3h, then add reverse micelle-enzyme, hydrolyze the raw meal at 55℃ for 60min, centrifuge at 2500r / min, and take the upper phase solution , Get starch paste;

[0060] The reverse micelle-enzyme composition is: 224IU / g cassava flour α-amylase and 694IU / g cassava flour glucoamylase;

[0061] The preparation method of the loaded ionic liquid biochar in step B is as follows:

[0062] (1) The biochar with a particle size of 420μm was reacted with ethanol solution, 1mol / L hydrochloric acid solution, and deionized water at a solid-to-liquid ratio of 1:2 under magnetic stirring for 4 hours at 20°C, filtered to remove or...

Embodiment 3

[0070] A. Crush the disease-free dry cassava flakes, cross 80 meshes to obtain cassava flour, then mix the cassava flour with 0.5g / L yeast extract, 0.12g / L urea, 1.5g / L peptone, and 25mg / L calcium sulfate. Mix in warm water at 65°C and prepare a 30% (W / V) tapioca solution;

[0071] B. Add 8 times the amount of loaded ionic liquid biochar into the tapioca powder solution for 3 hours, then add reverse micelle-enzyme, hydrolyze the raw meal at 50°C for 45 minutes, centrifuge at 2000r / min, and take the upper phase solution , Get starch paste;

[0072] The reverse micelle-enzyme composition is: 264IU / g cassava flour α-amylase and 392IU / g cassava flour glucoamylase;

[0073] The preparation method of the loaded ionic liquid biochar in step B is as follows:

[0074] (1) The biochar with a particle size of 350μm was reacted with ethanol solution, 1mol / L hydrochloric acid solution, and deionized water at a solid-to-liquid ratio of 1:2 at 20°C for 4 hours with magnetic stirring, filtered to re...

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Abstract

The invention provides a cassava raw material saccharification method and a novel process for producing ethanol by the method. Cassava flour is treated through loading of ion liquid by charcoal; the dissolving-out rate of cassava starch can be improved; then, reverse micelle-enzyme is used for starch hydrolysis; next, yeast is inoculated for fermentation so as to produce ethanol; through the loading of ion liquid by charcoal, the reaction efficiency is improved; the cost is reduced; meanwhile, the inhibition effect of thick mash on the yeast can be reduced; reverse micelles can be used for improving the enzymatic activity and the enzymatic stability; meanwhile, the reverse micelles can improve the ion liquid recovery rate. The ethanol saccharification process provided by the invention innovates the novel conventional high-temperature steaming and boiling saccharification; the energy consumption is reduced; the ethanol yield is improved; good development prospects are realized.

Description

Technical field [0001] The invention belongs to the technical field of alcohol production, and particularly relates to a new process for saccharification of raw cassava meal and alcohol production by the method. Background technique [0002] With the advent of the energy crisis, the use of renewable resources (grain or plant fiber) to ferment to produce ethanol has been put on the agenda as bioenergy to replace or partially replace petroleum. Fuel ethanol, as a clean energy source, has attracted more attention now that environmental protection is increasingly valued. Cassava is one of the three major potato types in the world, with a global annual output of more than 130 million tons. The starch content of dried cassava exceeds 80%, which is an ideal raw material for alcohol production. As the global energy crisis intensifies, the industry that uses cassava as a raw material to ferment and produce alcohol is gradually expanding. [0003] At present, with starch raw materials as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10
CPCC12P7/10C12P2201/00Y02E50/10
Inventor 罗绍庆
Owner 罗绍庆