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Method for quickly and sensitively detecting etomidate in blood

A technology for etomidate and blood is applied in the field of detecting etomidate in blood, which can solve the problems of inaccurate monitoring of etomidate blood concentration, time-consuming and laborious monitoring by HPLC, and inability to real-time online measurement, so as to ensure data repeatability. , The device is stable and avoids the effect of daytime difference

Inactive Publication Date: 2018-05-25
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

Etomidate plasma concentration monitoring abroad uses the HPLC method, and there are only some domestic reports. used, mostly in the stage of scientific research
Although gas chromatography (GC), high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (HPLC-MS) can monitor blood drug concentrations, they cannot be measured online in real time, and due to the huge equipment, Complicated sample pretreatment is required, time-consuming and other disadvantages. Even if the drug-related data is obtained in the laboratory, the anesthesia has long been over, so the above equipment cannot be used in clinical practice.
There is no report on monitoring etomidate plasma concentration with ion mobility spectrometer at home and abroad

Method used

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  • Method for quickly and sensitively detecting etomidate in blood
  • Method for quickly and sensitively detecting etomidate in blood

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The experimental conditions used in the experiment are: the ionization source is the ion mobility spectrum of lamp ionization, the positive ion mode high voltage source, and the stepping motor thermal analysis sampler. During the experiment, the temperature of the migration tube was kept at 100°C, the sampler was 200°C, and the flow of carrier gas (air) and bleaching gas (air) were 300 mL / min and 400 mL / min, respectively. The internal standard is DNP, 10ng / μl, the sample injection volume is 10μl, and 10μl of the internal standard is mixed and dried at room temperature (20-30℃).

[0031] figure 1 The ion mobility spectrum of etomidate detected in positive ion mode is given.

[0032] Such as figure 1 As shown, it can be known by ion mobility calibration. When the migration time of acetone is 3.24ms, the migration time of etomidate monomer peak is 4.64ms, and the migration time of internal standard DNP is 7ms. Follow-up tests use this as a standard for qualitative analysis.

Embodiment 2

[0034] The experimental conditions used in the experiment are: the ionization source is the ion mobility spectrum of lamp ionization, the positive ion mode high voltage source, and the stepping motor thermal analysis sampler. During the experiment, the temperature of the migration tube was kept at 100°C, the sampler was 200°C, and the flow of carrier gas (air) and bleaching gas (air) were 300 mL / min and 400 mL / min, respectively. The internal standard is DNP, 10ng / μl, the sample injection volume is 10μl, and 10μl of the internal standard is mixed and dried at room temperature (20-30℃). Etomidate was formulated into samples containing 0.5, 1, 2.5, 5, 12.5 ng / μl etomidate.

[0035] figure 2 The standard curve of etomidate quantitative analysis in positive ion mode is given.

[0036] According to the migration time of etomidate and the internal standard reagent, the ion mobility spectrum was continuously collected for 30 seconds, and the ratio of the peak intensity of the continuous ...

Embodiment 3

[0038] The experimental conditions used in the experiment are: the ionization source is the ion mobility spectrum of lamp ionization, the positive ion mode high voltage source, and the stepping motor thermal analysis sampler. During the experiment, the temperature of the migration tube was kept at 100°C, the sampler was 200°C, and the flow of carrier gas (air) and bleaching gas (air) were 300 mL / min and 400 mL / min, respectively. The internal standard is DNP, 10ng / μl, the sample injection volume is 10μl, and 10μl of the internal standard is mixed and dried at room temperature (20-30℃).

[0039] Determine the signal peak and internal standard peak of the etomidate drug in the blood sample to be tested according to the signal peak migration time obtained by the etomidate drug and the internal standard detection, and bring the maximum signal intensity ratio into the standard curve equation to further determine the blood The content of etomidate in the drug.

[0040] For example, an un...

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Abstract

The invention discloses a method for quickly and sensitively detecting tomidate in blood. The method uses ion mobility spectrometry as a basic detection technique, utilizes an ion mobility spectrometer as a detection means, adopts a positive-ion high-voltage mode, and combines a conventional liquid-liquid extraction pretreatment method and a high-temperature thermal desorption sample introductionway; an internal standard calibrating reagent is added in a sample introduction process. A device in the method is stable and reliable; the method is simple, convenient, quick and efficient. The detection and analysis time of a single sample is less than 30S. The detection is high in sensitivity; a measurement detection limit can reach 0.1ng / mul; a quantitative analysis concentration range is 0.5ng / mul to 20ng / mul. An established qualitative and quantitative analysis method meets a human-body administration concentration analysis range, to a patient, of a doctor. The detection method providedby the invention can be widely used for clinical administration deep analysis, and the doctor is conducted to clinically accurately medicate.

Description

Technical field [0001] The invention discloses a fast and sensitive method for detecting etomidate in blood. This method uses ion mobility spectrometry technology as the basic detection technology, uses ion mobility spectrometer as detection means, adopts positive ion high pressure mode, and combines traditional liquid-liquid extraction pretreatment methods and high-temperature thermal analysis sampling methods. The device in the invention is stable, reliable, and the method is simple, fast and efficient. The established qualitative and quantitative analysis method meets the doctor's analysis range of the clinical administration concentration of anesthetized patients. The detection method of the present invention can be widely used in clinical drug administration in-depth analysis to guide clinicians in precise drug use. Background technique [0002] Anesthesia is the use of anesthetic drugs to administer sedation, analgesia, hypnosis, muscle relaxation and other effects to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N1/34G01N1/38
CPCG01N1/34G01N1/38G01N27/622
Inventor 王新蒋丹丹李海洋李恩有肖瑶
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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