Method for detecting flavonoid components in peony leaves

A technology of peony leaves and flavonoids, which is applied in the field of research on components of peony leaves, to achieve the effect of simple operation steps, rapid detection and high sensitivity

Inactive Publication Date: 2018-05-29
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] However, at present, the analysis of flavonoid components in peony leaves is still relatively small, and many precious components in peony leaves have not been detected, and the method for the most comprehensive detection of flavonoid components in peony leaves has not been reported.

Method used

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  • Method for detecting flavonoid components in peony leaves
  • Method for detecting flavonoid components in peony leaves
  • Method for detecting flavonoid components in peony leaves

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Experimental program
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preparation example Construction

[0028] (1) Preparation of flavonoid extract: Grind peony leaves into peony leaf powder under freezing conditions, mix the peony leaf powder with an alcohol solution to obtain a mixture containing peony leaves, and then process the mixture in sequence Ultrasonic treatment and centrifugal treatment, collecting the supernatant liquid and removing the precipitate, and then filtering the collected supernatant liquid through a filter membrane to remove solid impurities to obtain a flavonoid extract;

[0029] (2) The chromatographic conditions of the ultra-high performance liquid chromatograph, the mass spectrometry conditions of the triple quadrupole time-of-flight tandem mass spectrometer, and the setting of the detection wavelength of the diode array detector;

[0030] (3) Using ultra-high performance liquid chromatography-diode array detector-triple quadrupole time-of-flight tandem mass spectrometry technology to detect flavonoid extracts to obtain detection results; and

[0031] (4) Th...

Embodiment 1

[0049] Grind 13 fresh peony leaves into powder under liquid nitrogen and weigh 300mg, add 900μL of methanol solution containing 0.1% by volume formic acid and 80% concentration to extract the flavonoids from peony leaves, vortex for 1min to mix, ultrasonic Extract for 30 min, centrifuge at a high speed of 13000 r / min for 10 min, collect the supernatant and remove the precipitate, and then pass the supernatant through a 0.22 μm organic filter membrane to remove solid impurities to obtain a flavonoid extract. The obtained flavonoid extract was subjected to ultra-high performance liquid chromatography-diode array detector-triple quadrupole time-of-flight tandem mass spectrometry technology analysis. Among them, the chromatographic conditions, mass spectrometry conditions, and the detection wavelength range of the diode array detector are set as follows:

[0050] The chromatographic conditions are: mobile phase: solvent A is a mixed solution of acetonitrile and water containing 0.1% ...

Embodiment 2

[0063] Embodiment 2 is basically the same as embodiment 1, except that:

[0064] The chromatographic conditions are: mobile phase: solvent A is a mixed solution of acetonitrile and water containing 0.2% by volume formic acid, the volume ratio of water to acetonitrile is 90:10, and solvent B is an acetonitrile solution containing 0.2% by volume formic acid; the flow rate is 0.2mL / min; column temperature is 30℃; sample volume is 4μL; gradient elution conditions: 0~22min, 100% solvent A~72% solvent A, 0% solvent B~28% solvent B; 22~22.5min, 72% solvent A~60% solvent A, 28% solvent B~40% solvent B; 22.5~23min, 60% solvent A~0% solvent A, 40% solvent B~100% solvent B; 23~26.5min, 0 % Solvent A, 100% Solvent B; 26.5~27min, 0% Solvent A~100% Solvent A, 100% Solvent B~0% Solvent B; 27~32min, 100% Solvent A, 0% Solvent B, that is liquid The gradient elution conditions of the phase chromatography were set according to the program in Table 1.

[0065] A total of 29 major flavonoids were id...

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Abstract

The invention relates to a method for detecting the flavonoid components in peony leaves. The method comprises the following steps of preparing a flavonoid extracting solution; setting chromatographicconditions, mass spectrum conditions and the detection wavelength of a diode array detector; adopting the super-efficient liquid chromatogram-diode array detector-triple quadrupole rod flight time tandem mass spectrum linkage technology for detecting the flavonoid extracting solution, and obtaining a detecting result; performing structure identification on flavonoid substances by analyzing the detecting result, and speculating the structure of the flavonoid compound. The method is simplified in operation steps, rapid in detection and high in accuracy and sensitivity, the micro content of theflavonoid components can be detected, the identification variety is comprehensive, 45 main flavone, flavonol, flavanone and flavanol compounds are identified in the peony leaves, a gap for flavonoid component identification in the peony leaves is filled up, and the method is of great significance in analysis of different varieties of peony leaf nutrient contents.

Description

Technical field [0001] The invention relates to a method for studying the components of peony leaves, in particular to a method for detecting flavonoid components in peony leaves by using ultra-high performance liquid chromatography-diode array detector-triple quadrupole time-of-flight tandem mass spectrometry technology. Background technique [0002] Flavonoids refer to the basic nucleus of 2-phenylchromone compounds, that is, a series of compounds in which two benzene rings with phenolic hydroxyl groups are connected to each other through three central carbon atoms. According to the difference in the basic molecular structure of flavonoids and the hetero-oxygen ring and conformation, flavonoids are generally divided into six categories: flavonoids, flavonols, isoflavones, flavanones, flavanols and anthocyanins. Flavonoids, also known as bioflavonoids, are the most abundant polyphenol compounds in the human diet. They are widely found in vegetables, fruits, tea, grapes and peoni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 薛璟祺杨德顺张秀新刘永森郑姝宁王顺利孙青文
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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