A method for detecting flavonoid components in peony leaves

A technology of peony leaves and flavonoids, which is applied in the field of research on components of peony leaves, achieves the effects of high accuracy, high sensitivity, and simple operation steps

Inactive Publication Date: 2020-09-15
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, at present, the analysis of flavonoid components in peony leaves is still relatively small, and many precious components in peony leaves have not been detected, and the method for the most comprehensive detection of flavonoid components in peony leaves has not been reported.

Method used

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  • A method for detecting flavonoid components in peony leaves
  • A method for detecting flavonoid components in peony leaves
  • A method for detecting flavonoid components in peony leaves

Examples

Experimental program
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Effect test

preparation example Construction

[0028] (1) Preparation of flavonoid extract: grind peony leaves into peony leaf powder under freezing conditions, mix the peony leaf powder with alcohol solution to obtain a mixture containing peony leaves, and then process the mixture in sequence Ultrasonic treatment and centrifugation, collecting the supernatant and removing the precipitate, and then filtering the collected supernatant through a filter membrane to remove solid impurities to obtain a flavonoid extract;

[0029] (2) The chromatographic conditions of the ultra-high performance liquid chromatography, the mass spectrometry conditions of the triple quadrupole time-of-flight tandem mass spectrometer and the setting of the detection wavelength of the diode array detector;

[0030] (3) Using ultra-high performance liquid chromatography-diode array detector-triple quadrupole time-of-flight tandem mass spectrometry to detect the flavonoid extract, and obtain the detection results; and

[0031] (4) Identifying the struc...

Embodiment 1

[0049] Grind 13 parts of fresh peony leaves into powder under liquid nitrogen, weigh 300 mg, add 900 μL of 80% methanol solution containing 0.1% formic acid to extract the flavonoid components in peony leaves, vortex for 1 min to mix, and ultrasonically Extract for 30 minutes, centrifuge at a high speed of 13000r / min for 10 minutes, collect the supernatant and remove the precipitate, then pass the supernatant through a 0.22 μm organic filter to remove solid impurities to obtain a flavonoid extract. The obtained flavonoid extract was analyzed by ultra-high performance liquid chromatography-diode array detector-triple quadrupole time-of-flight tandem mass spectrometry. Wherein, the settings of chromatographic conditions, mass spectrometry conditions and diode array detector detection wavelength range are as follows:

[0050] The chromatographic conditions are: mobile phase: solvent A is a mixed solution of acetonitrile and water containing 0.1% volume formic acid, the volume rat...

Embodiment 2

[0063] Embodiment 2 is basically the same as Embodiment 1, the difference is:

[0064] The chromatographic conditions are: mobile phase: solvent A is a mixed solution of acetonitrile and water containing 0.2% volume formic acid, the volume ratio of water and acetonitrile is 90:10, solvent B is an acetonitrile solution containing 0.2% volume formic acid; flow rate is 0.2mL / min; column temperature is 30°C; injection volume is 4μL; gradient elution conditions: 0-22min, 100% solvent A-72% solvent A, 0% solvent B-28% solvent B; 22-22.5min, 72% solvent A~60% solvent A, 28% solvent B~40% solvent B; 22.5~23min, 60% solvent A~0% solvent A, 40% solvent B~100% solvent B; 23~26.5min, 0 % solvent A, 100% solvent B; 26.5 ~ 27min, 0% solvent A ~ 100% solvent A, 100% solvent B ~ 0% solvent B; 27 ~ 32min, 100% solvent A, 0% solvent B, that is, liquid The gradient elution conditions of phase chromatography were set according to the procedures in Table 1.

[0065] A total of 29 main flavonoid...

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Abstract

The invention relates to a method for detecting the flavonoid components in peony leaves. The method comprises the following steps of preparing a flavonoid extracting solution; setting chromatographicconditions, mass spectrum conditions and the detection wavelength of a diode array detector; adopting the super-efficient liquid chromatogram-diode array detector-triple quadrupole rod flight time tandem mass spectrum linkage technology for detecting the flavonoid extracting solution, and obtaining a detecting result; performing structure identification on flavonoid substances by analyzing the detecting result, and speculating the structure of the flavonoid compound. The method is simplified in operation steps, rapid in detection and high in accuracy and sensitivity, the micro content of theflavonoid components can be detected, the identification variety is comprehensive, 45 main flavone, flavonol, flavanone and flavanol compounds are identified in the peony leaves, a gap for flavonoid component identification in the peony leaves is filled up, and the method is of great significance in analysis of different varieties of peony leaf nutrient contents.

Description

technical field [0001] The invention relates to a method for researching components of peony leaves, in particular to a method for detecting flavonoid components in peony leaves by using ultra-high performance liquid chromatography-diode array detector-triple quadrupole time-of-flight tandem mass spectrometry. Background technique [0002] Flavonoids refer to compounds whose basic core is 2-phenylchromone, that is, a series of compounds in which two benzene rings with phenolic hydroxyl groups are connected to each other through the central three carbon atoms. According to the basic molecular structure and conformation of flavonoids, flavonoids are generally divided into six categories: flavonoids, flavonols, isoflavones, flavanones, flavanols and anthocyanins. Flavonoids, also known as bioflavonoids, are the most abundant polyphenolic compounds in the human diet and are widely found in vegetables, fruits, tea, grapes and peonies. Related studies have shown that flavonoids s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 薛璟祺杨德顺张秀新刘永森郑姝宁王顺利孙青文
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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