Nanofiber membrane containing calcitonin lipidosome and preparation method and application thereof

A nanofibrous membrane and liposome technology, which is applied in liposome delivery, medical preparations containing active ingredients, fiber treatment, etc., can solve the problems of poor liposome stability and achieve low Zeta potential and excellent stability , The effect of expanding the application space

Active Publication Date: 2018-06-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the stability of liposomes is poor, so other m

Method used

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  • Nanofiber membrane containing calcitonin lipidosome and preparation method and application thereof
  • Nanofiber membrane containing calcitonin lipidosome and preparation method and application thereof
  • Nanofiber membrane containing calcitonin lipidosome and preparation method and application thereof

Examples

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Example Embodiment

[0036] Example 1

[0037] The method for preparing a nanofiber membrane containing calcitonin liposomes of this embodiment, the specific steps are as follows:

[0038] (1) Weigh 150 mg of soy lecithin and 25 mg of cholesterol, add them to a mixed solvent of ether-methanol (8:1, v / v), and rotate in a constant temperature water bath at 35°C until the organic solvent is completely removed, until the organic solvent is completely removed. A uniform lipid film is formed on the wall. Then, 4 ml of salmon calcitonin phosphate buffer solution was added to the obtained lipid film, soaked in a constant temperature water bath at 70° C. for 10 seconds, vortexed and eluted, and repeated 3 times to obtain a suspension. Incubate the resulting suspension in a water bath at 10°C for 1 hour, and then ultrasound the probe for 3 minutes (ultrasound power 200W, ultrasound 3s, 3s off) under ice-water bath conditions, and pass through a 0.22μm microporous membrane to obtain calcitonin nanolipids body. ...

Example Embodiment

[0045] Example 2

[0046] Compared with Example 1, the method for preparing a nanofiber membrane containing calcitonin liposomes of this embodiment is different in that the mass fraction of the sodium alginate aqueous solution is 2%, and the remaining steps and parameters are completely the same.

[0047] The SEM image and fiber diameter distribution of the nanofiber membrane containing calcitonin liposomes finally obtained in this example are as follows image 3 Shown.

Example Embodiment

[0048] Example 3

[0049] Compared with Example 1, the method for preparing a nanofiber membrane containing calcitonin liposomes of this embodiment is different in that the mass fraction of the sodium alginate aqueous solution is 3%, and the remaining steps and parameters are completely the same.

[0050] The SEM image and fiber diameter distribution of the nanofiber membrane containing calcitonin liposomes finally obtained in this example are as follows Figure 4 Shown.

[0051] 1. To investigate the release rate of the nanofiber membranes containing liposomes containing calcitonin in vitro under simulated gastrointestinal environment, the specific implementation steps are as follows:

[0052] Refer to the second appendix of the Chinese Pharmacopoeia 2010 edition of the second part of the in vitro release determination method (plasma method) and make appropriate modifications, the specific conditions are: temperature 37 ± 0.5 ℃, the release medium is simulated artificial gastric juice...

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Abstract

The invention belongs to the field of medicine slow release materials, and discloses a nanofiber membrane containing calcitonin lipidosome and a preparation method and application thereof. Soybean lecithin, cholesterol and organic solvent are subjected to rotary evaporation to obtain a lipoid membrane; then, calcitonin phosphate buffered solution is added to prepare naometer lipidosome carrying with calcitonin; the naometer lipidosome carrying with calcitonin is added into pectin solution to obtain coated lipidosome carrying with calcitonin pectin; the coated lipidosome carrying with calcitonin pectin is added into the mixed solution of polyvinyl alcohol and sodium alginate to prepare the membrane through electrostatic spinning so as to obtain the nanofiber membrane containing calcitonin lipidosome. According to the nanofiber membrane, the lipidosome carrying with calcitonin is added, pectin is used for coating, and therefore, the bioavailability and the stability of the calcitonin canbe improved.

Description

technical field [0001] The invention belongs to the field of drug sustained-release materials, and in particular relates to a nanofibrous membrane containing calcitonin liposome, a preparation method and application thereof. Background technique [0002] With the development of biochemistry and biotechnology, a large number of protein and polypeptide drugs with strong biological activity are emerging. This type of drug has the advantages of high activity, low dosage, and small adverse reactions. However, this type of drug also has a large relative molecular mass, most of which are water-soluble, short half-life, poor membrane permeability, easy to degrade and unstable, and are difficult for formulations. The shortcomings of high technical requirements greatly limit its clinical application. Therefore, research and development of new dosage forms and new technologies of protein and polypeptide drugs to improve drug stability, prolong its half-life, increase its membrane perm...

Claims

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Application Information

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IPC IPC(8): A61K9/70A61K38/23A61K9/127A61K47/36A61P19/10A61P19/08A61P29/00A61P3/14D04H1/4382D04H1/4309D01D5/00
CPCA61K9/127A61K9/7007A61K38/23A61K47/36D01D5/003D01D5/0061D01D5/0092D04H1/4309D04H1/4382
Inventor 吴虹李晨冯坤韦昀姗宗敏华
Owner SOUTH CHINA UNIV OF TECH
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