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Nanofiber membrane containing calcitonin lipidosome and preparation method and application thereof

A nanofibrous membrane and liposome technology, which is applied in liposome delivery, medical preparations containing active ingredients, fiber treatment, etc., can solve the problems of poor liposome stability and achieve low Zeta potential and excellent stability , The effect of expanding the application space

Active Publication Date: 2018-06-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the stability of liposomes is poor, so other methods are needed to improve the stability of liposomes

Method used

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  • Nanofiber membrane containing calcitonin lipidosome and preparation method and application thereof
  • Nanofiber membrane containing calcitonin lipidosome and preparation method and application thereof
  • Nanofiber membrane containing calcitonin lipidosome and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of preparation method of the nanofibrous membrane containing calcitonin liposome of the present embodiment, concrete steps are as follows:

[0038](1) Weigh 150 mg of soybean lecithin and 25 mg of cholesterol, add them into a mixed solvent of ether-methanol (8:1, v / v), and rotatively evaporate in a constant temperature water bath at 35°C until the organic solvent is completely removed, until A uniform lipid film forms on the wall. Then, 4 ml of salmon calcitonin phosphate buffer solution was added to the obtained lipid film, immersed in a constant temperature water bath at 70° C. for 10 seconds, vortexed and eluted, and repeated 3 times to obtain a suspension. The resulting suspension was incubated in a water bath at 10°C for 1 hour, then the probe was sonicated for 3 minutes in an ice-water bath (200W ultrasonic power, 3 seconds of ultrasound, 3 seconds off), and passed through a 0.22 μm microporous membrane to obtain calcitonin-loaded nanolipids. body. Slowly...

Embodiment 2

[0046] Compared with Example 1, the preparation method of a nanofibrous membrane containing calcitonin liposomes in this example is different in that the mass fraction of sodium alginate aqueous solution is 2%, and the rest of the steps and parameters are exactly the same.

[0047] The SEM figure and fiber diameter distribution of the nanofibrous membrane containing calcitonin liposome finally obtained in the present embodiment are as follows: image 3 shown.

Embodiment 3

[0049] Compared with Example 1, the preparation method of a nanofibrous membrane containing calcitonin liposomes in this example is different in that the mass fraction of sodium alginate aqueous solution is 3%, and the rest of the steps and parameters are exactly the same.

[0050] The SEM figure and fiber diameter distribution of the nanofibrous membrane containing calcitonin liposome finally obtained in the present embodiment are as follows: Figure 4 shown.

[0051] One, to the investigation of the release rate of the nanofibrous membrane containing calcitonin liposomes obtained by the invention under the in vitro simulated gastrointestinal tract environment, the specific implementation steps are as follows:

[0052] Refer to the second appendix of the Chinese Pharmacopoeia 2010 edition, the second method (slurry method) of in vitro release test method and make appropriate modifications, the specific conditions are: temperature 37±0.5°C, release medium is simulated artifici...

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Abstract

The invention belongs to the field of medicine slow release materials, and discloses a nanofiber membrane containing calcitonin lipidosome and a preparation method and application thereof. Soybean lecithin, cholesterol and organic solvent are subjected to rotary evaporation to obtain a lipoid membrane; then, calcitonin phosphate buffered solution is added to prepare naometer lipidosome carrying with calcitonin; the naometer lipidosome carrying with calcitonin is added into pectin solution to obtain coated lipidosome carrying with calcitonin pectin; the coated lipidosome carrying with calcitonin pectin is added into the mixed solution of polyvinyl alcohol and sodium alginate to prepare the membrane through electrostatic spinning so as to obtain the nanofiber membrane containing calcitonin lipidosome. According to the nanofiber membrane, the lipidosome carrying with calcitonin is added, pectin is used for coating, and therefore, the bioavailability and the stability of the calcitonin canbe improved.

Description

technical field [0001] The invention belongs to the field of drug sustained-release materials, and in particular relates to a nanofibrous membrane containing calcitonin liposome, a preparation method and application thereof. Background technique [0002] With the development of biochemistry and biotechnology, a large number of protein and polypeptide drugs with strong biological activity are emerging. This type of drug has the advantages of high activity, low dosage, and small adverse reactions. However, this type of drug also has a large relative molecular mass, most of which are water-soluble, short half-life, poor membrane permeability, easy to degrade and unstable, and are difficult for formulations. The shortcomings of high technical requirements greatly limit its clinical application. Therefore, research and development of new dosage forms and new technologies of protein and polypeptide drugs to improve drug stability, prolong its half-life, increase its membrane perm...

Claims

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Application Information

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IPC IPC(8): A61K9/70A61K38/23A61K9/127A61K47/36A61P19/10A61P19/08A61P29/00A61P3/14D04H1/4382D04H1/4309D01D5/00
CPCA61K9/127A61K9/7007A61K38/23A61K47/36D01D5/003D01D5/0061D01D5/0092D04H1/4309D04H1/4382
Inventor 吴虹李晨冯坤韦昀姗宗敏华
Owner SOUTH CHINA UNIV OF TECH
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