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Preparation method of biological enzyme-loaded anode with response to enzyme by temperature stimulation

A stimulus-response and bio-enzyme technology is applied in measuring devices, instruments, and material analysis through electromagnetic means. The relationship between performance is difficult to control and other problems, to achieve the effect of ensuring catalytic performance and stability, simple performance, and improving stability

Inactive Publication Date: 2018-06-08
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, most people prepare responsive films by drip-coating responsive polymers on the electrode surface, grafting polymers directly on the electrodes, or by free radical polymerization and electropolymerization on the electrode surfaces. The polymer films prepared by these methods It is difficult to control the thickness, grafting rate, and structure of polymer brushes, and the relationship between electrode stimulus responsiveness and electrode electrical properties is difficult to control

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  • Preparation method of biological enzyme-loaded anode with response to enzyme by temperature stimulation
  • Preparation method of biological enzyme-loaded anode with response to enzyme by temperature stimulation
  • Preparation method of biological enzyme-loaded anode with response to enzyme by temperature stimulation

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Experimental program
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Effect test

Embodiment 1

[0030] Implementation example 1: the reaction flow diagram is as figure 1 shown.

[0031] The steps of carbon paper surface modification:

[0032] Step 1: Cleaning of carbon paper: Ultrasound the hydrophilic carbon paper in anhydrous methanol for 50 minutes, rinse the surface of the carbon paper with ultrapure water (resistance 18.2MΩ), dry at room temperature, and set aside;

[0033] Step 2: Modification of carbon paper: Take a clean carbon paper as the working electrode, the platinum column electrode as the counter electrode, and the saturated silver / silver chloride electrode as the reference electrode to construct a three-electrode system. K for 8 2 HPO 4 -KH 2 PO 4 In the buffer solution, the stirring rate was 500rpm, and the cyclic voltammetry was used to scan firstly, the scanning potential range was -0.5~3V, and the scanning rate was 0.5V s -1 , scan the number of laps 20 laps, and then apply a constant voltage of -1V to the working electrode in the same solution ...

Embodiment 2

[0045] The steps of carbon paper surface modification:

[0046] Step 1: Cleaning of carbon paper: Ultrasound the hydrophilic carbon paper in anhydrous methanol for 10 minutes, rinse the surface of the carbon paper with ultrapure water (resistance 18.2MΩ), dry at room temperature, and set aside;

[0047] Step 2: Modification of carbon paper: Take a clean carbon paper as the working electrode, the platinum column electrode as the counter electrode, and the saturated silver / silver chloride electrode as the reference electrode to construct a three-electrode system. K for 6 2 HPO 4 -KH 2 PO 4 In the buffer solution, the stirring rate was 200rpm, and the cyclic voltammetry was used to scan firstly, the scanning potential range was -0.5~3V, and the scanning rate was 0.01V s -1 , scan the number of laps for 2 laps, and then apply a constant voltage of -1.2V to the working electrode in the same solution for 50s. After the completion, a carbon paper electrode with greatly improved c...

Embodiment 3

[0059] The steps of carbon paper surface modification:

[0060] Step 1: Cleaning of carbon paper: Ultrasound the hydrophilic carbon paper in anhydrous methanol for 45 minutes, rinse the surface of the carbon paper with ultrapure water (resistance 18.2MΩ), dry at room temperature, and set aside;

[0061] Step 2: Modification of carbon paper: Take a clean carbon paper as the working electrode, the platinum column electrode as the counter electrode, and the saturated silver / silver chloride electrode as the reference electrode to construct a three-electrode system. K for 6 2 HPO 4 -KH 2 PO 4 In the buffer solution, the stirring rate was 400rpm, and the cyclic voltammetry was used to scan firstly, the scanning potential range was -0.5~3V, and the scanning rate was 0.1V s -1 , scan the number of laps 10 laps, and then apply a constant voltage of -0.8V to the working electrode in the same solution for 100s. After the completion, a carbon paper electrode with greatly improved cond...

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Abstract

The invention relates to a preparation method of a biological enzyme-loaded anode with response to enzyme by temperature stimulation. The preparation method comprises the following steps of before electroplating of a gold layer, improving the electric conductivity and hydrophilcity of carbon paper by an electrochemical method, so as to uniformly plate gold onto the surface of carbon paper fiber, and obtain a porous and flexible electrode of which the cost is lower than the cost of the common gold electrode; controllably grafting a polymer chain segment onto the surface of the electrode by a surface-initiated atom transfer free radical polymerization method; immobilizing enzyme molecules to the surface of the electrode by covalent bonds. The preparation method has the advantages that the carbon paper with excellent heat conductivity and large specific surface area is used as a substrate material, so as to ensure the electrical property and chemical property of the electrode; after the used carbon paper substrate material is treated by an electrochemical method, the hydrophilcity is greatly enhanced, and the immobilization and stability of the biological enzyme are facilitated; the prepared electrode can be expected to be used as the anode which can be implanted into a body and be used for opening / closing an intelligent biological enzyme fuel battery.

Description

technical field [0001] The invention belongs to the technical field of preparation of electrocatalytic electrodes, and relates to a method for preparing a temperature-stimuli-responsive enzyme anode loaded with biological enzymes. Background technique [0002] Enzyme bioelectrodes are electrodes constructed with biologically active enzymes instead of noble metal catalysts. The electrode can catalyze the oxidation-reduction reaction of the substrate in the physiological body fluid environment, and convert the gain and loss of electrons into electrical signal output. The enzyme electrode can be used to construct an enzyme biosensor or an enzyme biofuel cell. A biosensor is an instrument that uses a biological substance as a substrate and converts its concentration into an electrical signal for detection. It has broad application prospects in food, pharmaceuticals, chemicals, clinical testing, biomedicine, and environmental monitoring. Enzyme biofuel cells are currently the m...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/327G01N27/48
Inventor 马晓燕屈枫锦惠昱晨索鸿飞
Owner NORTHWESTERN POLYTECHNICAL UNIV
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