Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of cinnamon camphor tissue culture method and culture medium thereof

A tissue culture and culture medium technology, applied in the directions of horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems such as browning of camphor tissue and low rooting rate, and achieve less browning, simple equipment, and browning. reduced effect

Active Publication Date: 2021-07-30
FUJIAN YONGAN FORESTRY GRP CO LTD
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problems of browning and low rooting rate in the tissue culture process of Cinnamomum camphora need to be further studied

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1.1 Cinnamomum camphora clone explants induced larval culture.

[0046] 1.1.1 Select mature trees with excellent genetic phenotypes as female parents for tissue culture. During the period from March to October, the explants were disinfected in the afternoon on a sunny day. From the base of the excellent plants, the main branches of the current year were selected as the explants from the main branch with a high degree of semi-lignification.

[0047] 1.1.2 After cutting off the leaves of the explants, cut them into stem segments with 1 to 2 axillary buds. Soak in 5g / L sterilized net or 84 disinfectant for 10 minutes, and rinse in running water for 0.5-1 hour.

[0048] 1.1.3 Perform the following operations under sterile conditions: (1) After washing twice with sterile water, disinfect with 75% alcohol for 30 seconds, and rinse with sterile water for three times; (2) Wash with 0.1% HgCl 2 The solution was shaken and soaked for 3 minutes, rinsed with sterile water for 3 t...

Embodiment 2

[0065] 2.1 Cinnamomum camphora clone explants induced larval culture.

[0066] 2.1.1 Select mature trees with excellent genetic phenotypes as female parents for tissue culture. During the period from March to October, the explants were disinfected in the afternoon on a sunny day. From the base of the excellent plants, the main branches of the current year were selected as the explants from the main branch with a high degree of semi-lignification.

[0067] 2.1.2 After cutting off the leaves of the explants, cut them into stem segments with 1 to 2 axillary buds. Soak in 5g / L sterilized net or 84 disinfectant for 10 minutes, and rinse in running water for 0.5-1 hour.

[0068] 2.1.3 Perform the following operations under sterile conditions: (1) After washing twice with sterile water, disinfect with 75% alcohol for 30 seconds, and rinse with sterile water for three times; (2) Wash with 0.1% HgCl 2 The solution was shaken and soaked for 3 minutes, rinsed with sterile water for 3 t...

Embodiment 3

[0085] 3.1 Induction of larvae culture by explants of Cinnamomum camphora clones.

[0086]3.1.1 Select mature trees with excellent genetic phenotypes as female parents for tissue culture. During the period from March to October, the explants were disinfected in the afternoon on a sunny day. From the base of the excellent plants, the main branches of the current year were selected as the explants from the main branch with a high degree of semi-lignification.

[0087] 3.1.2 After cutting off the leaves of the explants, cut them into stem segments with 1 to 2 axillary buds. Soak in 5g / L sterilized net or 84 disinfectant for 10 minutes, and rinse in running water for 0.5-1 hour.

[0088] 3.1.3 Perform the following operations under sterile conditions: (1) After washing twice with sterile water, disinfect with 75% alcohol for 30 seconds, and rinse with sterile water for three times; (2) Wash with 0.1% HgCl 2 The solution was shaken and soaked for 3 minutes, rinsed with sterile wa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a kind of Cinnamomum camphora tissue culture method and culture medium thereof, comprise following culture medium: the induction culture medium of bud is MS+0.5-1.2mg L ‑1 6‑BA+0.1‑0.2mg L ‑1 NAA+sucrose 30g·L ‑1 +Agar 5g·L ‑1 , the initial pH value is 5.4‑5.8; the formula of the bud subculture proliferation medium: 2 / 3MS+0.4‑1.0mg L ‑ 1 6‑BA+0.1‑0.2mg L ‑1 KT+0.1-0.2mg·L ‑1 NAA+sucrose 30g·L ‑1 +Agar 5g·L ‑1 The initial pH value is 5.4‑5.8; the formula of rooting medium: improved MS+0.2‑0.6mg L ‑1 IBA+0.4-0.8mg·L ‑1 ABT+0.2-0.6mg·L ‑1 NAA+sucrose 20g·L ‑1 + agar 6g·L ‑1 The initial pH value is 5.4-5.8; through explant selection, disinfection, bud induction, bud subculture proliferation culture, rooting culture, bottle seedling hardening, and transplanting, the tissue culture seedlings are obtained. The present invention can form bud clumps after 3 subcultures in the bud induction stage. In the bud subculture propagation stage, Cinnamomum camphora proliferates fast, grows fast, and browns less. , The plants with stretched leaves are rooted and cultivated, and the rooting rate is more than 90%.

Description

technical field [0001] The invention relates to a method for culturing cinnamon camphor tissue and its culture medium. Background technique [0002] Cinnamomum camphora (Cinnamomum camphora), belonging to the genus Lauraceae, is a subtropical evergreen broad-leaved tree, and its suitable environment is 10 degrees north latitude. ο to 30 ο Among them, it is a weakly positive tree species that likes warm, hot and humid climate conditions and is not resistant to severe cold. It is mainly distributed in Zhejiang, Fujian, Hunan, Hubei, Jiangxi, Guangdong, Yunnan and Taiwan provinces in my country. It is a precious timber tree species and aromatic plant in China. , occupies an important position in forestry production. [0003] Linalool is the one with the highest content of linalool in the branches and leaves of the five natural types of camphor. The huge system of linalool aroma raw materials and its derivatives has been widely used in the aroma industry, and linalool is the m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴炜
Owner FUJIAN YONGAN FORESTRY GRP CO LTD