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Preparation method of exosomes of deer placenta and application

A technology of exosomes and deer placenta, applied in the field of exosomes, can solve the problem of secrecy of sheep placenta, and achieve the effects of reducing scar formation, accelerating healing, and promoting the speed of proliferation.

Active Publication Date: 2018-06-12
HUBEI WEILAIJIAYUAN HIGH TECH AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of sheep placenta skin care products has been recognized by the world, but the ingredients of sheep placenta are still kept secret

Method used

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  • Preparation method of exosomes of deer placenta and application
  • Preparation method of exosomes of deer placenta and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Extraction of Deer Placental Exosomes and Preparation of Lyophilized Powder

[0025] Take healthy fresh deer placenta, soak in normal saline, and crush into a homogenate with a tissue homogenizer, and carry out the following steps:

[0026] (1) Centrifuge the homogenate for 30 minutes, collect the supernatant, and use the precipitated part for other purposes. The centrifugal force is 3000 g;

[0027] (2) centrifuge the supernatant of step (1) for 100min, collect the supernatant, and use the precipitation part for other purposes, and the centrifugal force is 10000g;

[0028] (3) centrifuge the supernatant of step (2) for 100 min, discard the supernatant, and collect the precipitate, and the centrifugal force is 100,000 g;

[0029] (4) After adding the precipitation of step (3) into PBS for remixing, centrifuging for 120 min, discarding the supernatant, collecting the precipitation, and suspending the precipitation with PBS, and the centrifugal force is 120000 ...

Embodiment 2

[0033] Example 2 Preparation of medicine for treating injury in the form of water preparation containing deer placental exosomes

[0034] The main components (w / v) of the water preparation are: 1-5% glycerol, 1-10% propylene glycol, 1-2% Tween, 0.1% ethyl paraben as thickener, dissolved in 60ml PBS buffer. Then add 10-50μg of deer placental exosome lyophilized powder, add 5ml of 0.01M phosphate buffer, mix and then add PBS to a final volume of 100ml, filter sterilization and insoluble matter through a 0.45μm filter, and complete the preparation in the form of water preparation. Process for the preparation of products containing deer placental exosomes.

[0035] The prepared deer placenta-containing exosome water can be used as a spray, lotion, etc., and can be sprayed or painted on the damaged part. The exosomes are in contact with the wound surface to release cytokines to accelerate the nutrition of the skin. Update and fix purpose.

Embodiment 3

[0036] Example 3 Preparation of products in emulsion form containing deer placental exosomes

[0037] The product in emulsified form can be prepared by different methods of oil-in-water or water-in-oil, such as 10% glycerol, 2% Tween 80, 2% cholesterol, 5% lecithin, 0.2% ethylparaben and 70ml PBS buffer Mix and heat for homogenization and emulsification, cool down to 37-45°C, add 10-50μg of deer placental exosome lyophilized powder, add 5ml of 0.01M phosphate buffer, mix and add water to a final volume of 100ml, homogenize and filter 0.45μm Membrane filtration to sterilize and insoluble matter to complete the preparation process of a product containing deer placental exosomes in the form of an emulsion formulation.

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Abstract

The invention discloses a preparation method of exosomes of deer placentas and application thereof and belongs to the technical field of biology. The preparation method and the application have the advantages that the high-purity exosomes are obtained by preparing living bodies of healthy-deer placentas into uniform slurry and carrying out differential centrifugal separation and low-temperature overspeed purification; good treating effect is obtained in the experimental treatment of the animal skin injury; the exosomes can be prepared into freeze-dried powder which can be stored for long term;the freeze-dried powder can be matched with different auxiliary materials to form different preparation products so as to have an excellent market prospect and clinical value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to bioactive exosomes extracted from healthy fresh deer placenta and applications thereof. Background technique [0002] Deer fetus is a traditional and classic traditional Chinese medicine. For thousands of years, Chinese gynecology and gynecology have been using it to regulate women's menstruation and maintain their appearance. According to "Compendium of Materia Medica", it is recorded that the deer fetus is warm in nature, sweet and salty in taste, invigorating the kidney and strengthening yang, invigorating the deficiency and producing sperm, regulating menstruation, nourishing the face and detoxifying, and has the functions of replenishing the essence and blood and restoring Shaoyang. . The Qing Dynasty's "Ben Cao Xin Bian" discusses in more detail "Deer fetus, replenishing essence and blood, for kidney deficiency, weakness, lack of essence and blood, women's deficien...

Claims

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Application Information

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IPC IPC(8): A61K35/50A61P17/00A61P17/02A61K8/98A61Q19/00A61Q19/02
CPCA61K8/982A61K35/50A61K2800/84A61Q19/00A61Q19/02
Inventor 尹维坊敖明章赵新红
Owner HUBEI WEILAIJIAYUAN HIGH TECH AGRI CO LTD
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