Construction method of photoelectrochemical aptasensor using titanium dioxide-molybdenum disulfide-gold ternary composite as scaffold
An aptamer sensor and composite material technology, which is applied in the fields of material electrochemical variables, scientific instruments, analytical materials, etc., can solve the problems of high detection limit, narrow linear range, poor stability, etc., achieve smooth surface, improve sensitivity, Good dispersion effect
Active Publication Date: 2019-09-27
HENAN UNIVERSITY
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Problems solved by technology
The present invention overcomes the shortcomings of narrow linear range, high detection limit and poor stability in the prior art, and no related reports have been seen so far
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Embodiment 1
[0034] A TiO 2 The nanosphere composite nanomaterial is the construction method of the photoelectrochemical aptasensor of loading support, and it comprises the following steps:
[0035] ①TiO 2 -MoS 2 -Preparation of Au ternary composite nanomaterials:
[0036] Anatase TiO 2 Preparation of nanospheres:
[0037] Weigh 1.75 g of hexadecylamine (HDA), and disperse it in 200 mL of absolute ethanol by stirring. After the dispersion is complete, add 0.80 mL of 0.1 M KCl aqueous solution and 0.51 mL of ultrapure water, and add 4.32 mL titanium isopropoxide (TIP), i.e. keep the molar ratio of HDA:ultrapure water:KCl:absolute ethanol:TIP as 0.5:5:5.5×10 -3 : 236.5:1.0. After continuing to stir for 30 min, let it stand for 18 h, centrifuge to collect the white precipitate, wash with absolute ethanol, and dry at 60 °C for 4 h to obtain the smooth surface amorphous TiO 2 Nanospheres, collected for later use;
[0038] In order to obtain anatase TiO with rough surface 2 Nanospheres,...
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Abstract
The invention relates to a construction method of a photo-electrochemical aptamer sensor adopting a TiO2-MoS2-Au ternary composite as a holder. The method comprises steps as follows: firstly, a TiO2 ball template is prepared with a sol-gel method, anatase TiO2 balls with rough surfaces are prepared from the TiO2 ball template, then MoS2 nanosheets are tightly loaded to the surfaces of the TiO2 balls with a hydrothermal method, and a TiO2-MoS2 heterojunction structure is formed; finally, Au nano-particles are deposited on the surface of the heterojunction structure with a trisodium citrate reduction method, and the TiO2-MoS2-Au ternary composite is prepared. The ternary composite has large specific surface area and good biocompatibility, the loading capacity of biomolecules is greatly increased, and the ternary composite further has excellent electrical conductivity and higher photoelectric conversion efficiency, thereby being suitable for constructing a biosensor. DNA aptamers are immobilized on the surface of the TiO2-MoS2-Au ternary composite, and research proves that the photo-electrochemical aptamer sensor prepared with the method can rapidly detect kanamycin and is higher in sensitivity, larger in linear range and lower in limit of detection.
Description
technical field [0001] The invention belongs to the technical field of photoelectrochemical aptamer sensor construction, in particular to a TiO 2 -MoS 2 -Au ternary composite material is a construction method of a photoelectrochemical aptamer biosensor loaded with a scaffold, and the photoelectrochemical aptamer sensor can be used to detect kanamycin. Background technique [0002] As a class of anti-infection drugs, antibiotics are mainly used to inhibit bacterial infection, kill pathogenic bacteria or other active substances, and are widely used in the fields of medicine, agriculture, forestry, aquaculture and animal husbandry. There are many kinds of antibiotics, and kanamycin is one of the commonly used basic aminoglycoside antibiotics, which has the characteristics of strong water solubility, wide antibacterial spectrum, obvious bactericidal effect, cheap price and convenient medication. However, irrational use of antibiotics can lead to enhanced drug resistance, endot...
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IPC IPC(8): G01N27/327G01N27/30
Inventor 刘小强刘培培唐云飞徐俊李乐乐杨立威
Owner HENAN UNIVERSITY