Activated partial thromboplastin time detection reagent and detection method

Abstract

The invention relates to the technical field of biology and especially relates to an activated partial thromboplastin time detection reagent and a detection method. The detection reagent is composed of: an activator, phospholipid, a bivalent metal ion salt, a buffer reagent, a surfactant, an antioxidant, and a stabilizer. The reagent can accurately and quickly detect the APTT and is good in stability and is stable even after preservation at 37 DEG C for 15 days without influence on detection effect. Compared with a control group without the surfactant, the detection reagent can accurately detect a sample in low fibrinogen content, so that detection limit is reduced and sensitivity is increased. The reagent is high in precision in detection on various samples, cv value being lower than 3%.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to a reagent and a method for detecting activated partial thromboplastin time. Background technique [0002] Activated partial thromboplatin time (APTT) is a screening test to check endogenous coagulation factors, which can be used to confirm the defects of congenital or acquired coagulation factors Ⅷ, Ⅸ, Ⅺ or whether they are corresponding Inhibitors. At the same time, APTT can also be used to confirm whether coagulation factor XII, prokallikrein and high molecular weight prokallikrein are lacking. Due to the high sensitivity of APTT and the way of action of heparin is mainly the endogenous coagulation pathway, APTT has become the first choice for monitoring unfractionated heparin. [0003] The activated partial thromboplastin time (APTT) test reagent is mainly composed of three parts: activator, phospholipid and calcium ion. During the test, the activator is mixed with plasma and i...

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Problems solved by technology

[0004] At present, the activators for APTT determination mainly include kaolin, diatomaceous earth, etc., which are natural mineral materials, but the reagents usually exist in the state of suspension, and the uniformity of the reagents is difficult to guarantee. If precipitation occurs, it will directly affect the accuracy of the test.

At present, most APTT reagents in China are in the form of freeze-dried powder preparations. The reagents need to be reconstituted before use. The operation is cumbersome, and it is easy to cause errors in the measurement results due to the inaccurate volume of the reconstituted solution.

As a common activator, ellagic acid is also widely used in the preparation of activated partial thromboplastin time assay reagents. Compared with activators such as kaolin and diatomaceous earth, ellagic acid has better solubility in water and is easy to It is made into a liquid reagent, but ellagic acid itself is a polyphenolic compound, which is easily oxidized, so it is easy to cause prolonged coagulation time

Moreover, when many commercially available reagents test samples with very low fibrinogen content, it is easy to fail to accurately read the clotting time because of the low reaction intensity.

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