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Bacillus subtilis, inoculant containing the same and application thereof, vomitoxin degradation method and kit

A technology of Bacillus subtilis and vomitoxin, which is applied in the field of microorganisms, can solve the problems that the degradation rate needs to be improved, the scope of application of biological methods is limited, etc., and achieves the effects of efficient and rapid degradation, good application prospects, and expansion of the scope of application.

Active Publication Date: 2018-07-06
COFCO NUTRITION & HEALTH RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent application CN103243047A discloses a strain of Bacillus subtilis that efficiently degrades deoxynivalenol and its application. 900 μL of Bacillus subtilis ANSB471 fermentation broth is reacted with 100 μL of deoxynivalenol (100 μg / ml), and the degradation rate of deoxynivalenol for 2 hours is 25 %, the degradation rate of vomitoxin in 24 hours of reaction is 56%, and the degradation rate of vomitoxin in 48 hours of reaction is 80%, and the degradation rate still needs to be improved
[0005] In addition, most of the existing biological methods for degrading vomitoxin are carried out under mild conditions (such as a temperature of 25-37°C and not exceeding 40°C, and a pH of about 7), however, under higher temperature loads (such as During transport in containers or during feed pelleting) or harsh acidic and alkaline conditions, there is no better solution, which limits the scope of application of biological methods in the degradation of vomitoxin

Method used

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  • Bacillus subtilis, inoculant containing the same and application thereof, vomitoxin degradation method and kit
  • Bacillus subtilis, inoculant containing the same and application thereof, vomitoxin degradation method and kit
  • Bacillus subtilis, inoculant containing the same and application thereof, vomitoxin degradation method and kit

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Embodiment

[0045] The present invention will be described in detail below by way of examples.

preparation example

[0046] In the following preparation examples, preparation comparative examples, embodiments and comparative examples:

[0047] Deoxynivalenol standard was purchased from Sigma Company, and other experimental materials used were purchased from conventional biochemical reagent stores unless otherwise specified.

[0048] LB liquid medium: 10g peptone, 5g yeast powder, 10g sodium chloride, dilute to 1L with deionized water, adjust pH to 7.

[0049] LB solid medium: 10g peptone, 5g yeast powder, 10g sodium chloride, 16g agar powder, dilute to 1L with deionized water, adjust pH to 7.

[0050] The bacterial strain provided by the present invention is Bacillus subtilis (Bacillus subtilis), and was preserved on October 24, 2016 in the General Microbiology Center of China Microbial Strain Collection Management Committee (Address: No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing , Institute of Microbiology, Chinese Academy of Sciences, postal code: 100101) (the abbr...

preparation example 1

[0057] After activating the Bacillus subtilis with the preservation number CGMCC NO.13139 provided by the present invention, inoculate the LB liquid medium sterilized at 121°C for 15 minutes with an inoculum size of 1% by volume, and culture with shaking at 160rpm and 37°C , the cultivation time makes the bacterial concentration in the bacterial liquid be (1±0.1)×10 5 CFU / mL. At the same time, it is made into dry powder, and the total number of live bacteria contained in each gram of dry powder is (1±0.1)×10 5 CFU.

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Abstract

Relating to the field of microorganisms, the invention discloses a Bacillus subtilis, an inoculant containing the same and application thereof, a vomitoxin degradation method and a kit. Specifically,the invention provides a Bacillus subtilis strain with a preservation number of CGMCC NO.13139. The Bacillus subtilis provided by the invention can efficiently and quickly degrade vomitoxin in grain and oil and / or feed, especially, when the vomitoxin content of grain and oil and / or feed is up to 50ppm or above, the Bacillus subtilis also can efficiently and quickly degrade vomitoxin, and has no influence to the palatability of rain and oil and / or feed. At the same time, adding of the Bacillus subtilis into vomitoxin polluted grain and oil and / or feed is safe. Therefore, the Bacillus subtilis has good application prospects.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a strain of Bacillus subtilis, a bacterium containing the bacterium and its application, a method for degrading vomitoxin and a kit. Background technique [0002] Vomitoxin (vomitoxin), also known as deoxynivalenol (deoxynivalenol, DON), the chemical name is 3α, 7α, 15-trihydroxy Fusarium-9-en-8-one, because it can cause pigs Named after vomiting, it is mainly trichothecene toxins produced by Fusarium graminearum and Fusarium culmorum infecting wheat, barley, oats, corn and other grains. Worldwide, vomitoxin is one of the main contaminating mycotoxins in grain, feed, and food, seriously affecting the health of humans and livestock. After humans and animals ingest food contaminated by vomitoxin, it will cause acute poisoning symptoms such as anorexia, vomiting, diarrhea, fever, unsteady standing, and unresponsiveness. In severe cases, it will damage the hematopoietic system and cause deat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L5/20A23K10/18C12R1/125
CPCC12N1/20C12N1/205C12R2001/125
Inventor 苏会波林海龙谭剑熊强李凡陈博朱镜羲
Owner COFCO NUTRITION & HEALTH RES INST