A kind of preparation method and application of epa plasmalogen

A technology of plasmalogen and plasmalogen phosphatidylcholine, applied in the field of improving or treating atherosclerosis products, prevention, preparation of sea cucumber EPA plasmalogen, which can solve the digestion and absorption of ethyl ester type DHA/EPA Difficulty, safety, different functions and activities, etc.

Active Publication Date: 2020-05-22
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In 2004, the U.S. State Food and Drug Administration (FDA) announced: "Containing EPA ingredients are qualified health foods that can reduce the risk of cardiovascular diseases", and ethyl ester EPA has been used in prescription drugs for the treatment of hyperlipidemia , but ethyl ester type DHA / EPA is difficult to digest and absorb in the body and safety issues have always been controversial
However, due to the difference in the type of the ester bond at the sn-1 position and the polar group at the sn-3 position in the phospholipid structure, it has different efficacy and activity, especially the plasmalogen with an ether bond at the sn-1 position, which is effective for atherosclerosis There is no report on the improvement of sclerotic cardiovascular diseases

Method used

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  • A kind of preparation method and application of epa plasmalogen

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Preparation of EPA plasmalogen

[0025] (1) Grinding commercially available North Atlantic ginseng into powder (200 meshes) after vacuum freeze-drying and crushing;

[0026] (2) Take 10kg of dry sea cucumber powder, add 100L chloroform / methanol (2:1, v / v), stir, filter, extract with 0.88% potassium chloride solution, let it stand for layers, collect the chloroform layer, and extract 3 Once, the concentrated solution was combined and concentrated under reduced pressure to obtain the sea cucumber lipid crude extract;

[0027] (3) Weigh 200g of silica gel, add 5% water, activate at 120°C for 4 hours, and cool at room temperature; use a wet method to pack the column, let it stand until the silica gel settles completely, and press the column with 2 times the column volume of chloroform to make the column The silica gel is denser; take 50g of the sea cucumber crude extract sample and dissolve it with as little chloroform as possible, transfer the solution with ...

Embodiment 2

[0030] Embodiment 2: animal experiments

[0031] 1. Materials and Methods

[0032] (1) Animal feeding

[0033] ApoE - / - Mice, male, 9-10 weeks old, were purchased from Nanjing Qingzilan Technology Co., Ltd. The humidity in the animal room was 65±15%, the room temperature was 23±2°C, light and dark alternated at 12:12 h, and the mice were free to eat and drink. After one week of adaptive feeding, the mice were divided into 4 groups according to body weight: model control group, EPA-EE, EPA-pPC, and EPA-pPE groups, with 8 mice in each group, and were fed with corresponding feeds. The feed was modified according to the AIN-93M rodent feed formula. The experimental group was supplemented with 1% EPA plasmalogen. The food intake was recorded and counted every day, and the weight change was recorded every other day. The feeding was continued for 8 weeks.

[0034] (2) Analysis of atherosclerotic plaque area

[0035] After the mouse liver was removed, the right atrial appendage w...

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PUM

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Abstract

The invention provides a preparation method and application of eicosapentaenoic acid (EPA) plasmalogen. According to the preparation method, the EPA plasmalogen is prepared by extracting from sea cucumber, and experiments prove that the EPA plasmalogen of all components has an improvement effect on atherosclerosis. The EPA plasmalogen is prepared by taking the sea cucumber as a raw material, and then separating the components; and the purities of the obtained pPC and pPE are 90% or above. The animal experiments prove that the EPA plasmalogen of all the components can effectively reduce the area of atherosclerotic plaque, that is, the EPA plasmalogen can significantly inhibit the development of atherosclerosis, showing that the EPA plasmalogen can effectively prevent, ameliorate or treat the atherosclerosis.

Description

technical field [0001] The invention belongs to the technical field of active substance screening, and relates to a preparation method and application of a plasmalogen, in particular to a preparation method of a sea cucumber EPA plasmalogen and its application in products for preventing, improving or treating atherosclerosis. Background technique [0002] Ether phospholipids are special phospholipids with ether bonds at the sn-1 position of the glycerol skeleton. There are two types of ether bonds, one contains only ether bonds and is called ether phospholipids; the other has ether bonds in addition to ether bonds. A phospholipid containing a double bond, which is an ether bond, is called a plasmalogen. Plasmalogens are the major phospholipid constituents in the brain, heart, and nerves, and evidence is accumulating that plasmalogen deficiency in tissues is associated with a variety of diseases. However, there is little direct evidence to prove the metabolism and biological...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F9/10A61K31/685A61P9/10
CPCA61K31/685C07F9/103
Inventor 王玉明丁琳薛长湖薛勇李兆杰徐杰常耀光王静凤唐庆娟
Owner OCEAN UNIV OF CHINA
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