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Method used for extracting sea-buckthorn active polypeptides with supersonic wave

An active polypeptide and ultrasonic technology, applied in the field of polysaccharides, can solve the problems of long time and low extraction efficiency, and achieve the effects of short production time, improved extraction rate and high purity

Inactive Publication Date: 2018-07-13
磐安县派普特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this polypeptide preparation method takes a long time and the extraction efficiency is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for ultrasonically extracting seabuckthorn active polypeptides, comprising preparation of seabuckthorn superfine powder, pretreatment of seabuckthorn superfine powder, ultrasonic-assisted extraction, separation and purification, and the specific preparation steps are:

[0015] 1) Preparation of seabuckthorn ultrafine powder: add glucopyranose and galactopyranose to seabuckthorn seeds, mix well, initially pulverize to 80 mesh, and then ultrafinely pulverize to 500 mesh to obtain seabuckthorn ultrafine powder, which is used for later use. The above glucopyranose The addition of glucopyranose and galactopyranose is 0.24‰ and 0.1‰ of the weight of seabuckthorn seeds respectively. The addition of glucopyranose and galactopyranose can prevent the denaturation of seabuckthorn protein during ultrafine grinding, reduce the loss of seabuckthorn protein, and The presence of glucopyranose and galactopyranose can assist phytase and tannase to quickly identify the position of...

Embodiment 2

[0020] A method for ultrasonically extracting seabuckthorn active polypeptides, comprising preparation of seabuckthorn superfine powder, pretreatment of seabuckthorn superfine powder, ultrasonic-assisted extraction, separation and purification, and the specific preparation steps are:

[0021] 1) Preparation of seabuckthorn ultrafine powder: add glucopyranose and galactopyranose to seabuckthorn seeds, mix well, initially pulverize to 40 mesh, and then ultrafinely pulverize to 700 mesh to obtain seabuckthorn ultrafine powder, which is used for later use. The above-mentioned glucopyranose and galactopyranose are added in amounts of 0.2‰ and 0.15‰ of seabuckthorn seeds weight respectively;

[0022] 2) Seabuckthorn superfine powder pretreatment: add distilled water to seabuckthorn superfine powder according to the material-to-liquid ratio of 1:5 (g / mL), mix well, adjust the pH to 5, and then add cellulase according to the enzyme amount of 0.8% , Phytase and tannase, hydrolyzed for ...

Embodiment 3

[0026] A method for ultrasonically extracting seabuckthorn active polypeptides, comprising preparation of seabuckthorn superfine powder, pretreatment of seabuckthorn superfine powder, ultrasonic-assisted extraction, separation and purification, and the specific preparation steps are:

[0027] 1) Preparation of seabuckthorn ultrafine powder: add glucopyranose and galactopyranose to seabuckthorn seeds, mix well, initially pulverize to 50 mesh, and then ultrafinely pulverize to 600 mesh to obtain seabuckthorn ultrafine powder, which is used for future use. The above glucopyranose and galactopyranose were added in amounts of 0.22‰ and 0.12‰ of seabuckthorn seed weight respectively;

[0028] 2) Seabuckthorn superfine powder pretreatment: add distilled water to seabuckthorn superfine powder according to the ratio of solid to liquid at 1:8 (g / mL), mix well, adjust the pH to 4.5, and then add cellulase according to the enzyme amount of 1.0% , phytase and tannase, hydrolyzed for 1.5h, ...

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PUM

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Abstract

The invention discloses a method used for extracting sea-buckthorn active polypeptides with supersonic wave. The method comprises following steps: glucopyranose and galactopyranose are added into sea-buckthorn seeds, and primary crushing and superfine grinding are carried out so as to obtain sea-buckthorn ultra-fine powder; distilled water, cellulase, phytase, and tannase are added into the sea-buckthorn ultra-fine powder, hydrolysis, enzyme deactivation, and centrifugation are carried out, a precipitate is collected, and is dried; distilled water, alkali protease, neutral protease, and left lateral vitamin C are added into sea-buckthorn powder, ultrasonic-assisted extraction and enzyme deactivation are carried out, an obtained enzymatic hydrolysate is cooled, and is subjected to ultrafiltration so as to obtain a sea-buckthorn active polypeptide crude solution; the sea-buckthorn active polypeptide crude solution is introduced into a chromatographic column for elution with double distilled water, an obtained eluate is introduced into a macroporous resin column for elution with double distilled water and an ethanol solution successively, an obtained elution solution is dried so as toobtain the sea-buckthorn active polypeptides. The method is simple and practical; pretreatment time is short; the purity of the sea-buckthorn active polypeptides is high; extraction rate is high; andthe obtained sea-buckthorn active polypeptides possess antibacterial performance and water retention capacity.

Description

technical field [0001] The invention relates to the technical field of polysaccharides, in particular to a method for ultrasonically extracting seabuckthorn active polypeptides. Background technique [0002] sea ​​buckthorn ( Sea Buckthorn ), also known as vinegar willow, is a deciduous shrub of the genus Seabuckthorn in the family Echinaceae. Domestically distributed in North China, Northwest, Southwest and other places. Seabuckthorn is a medicinal and edible plant. The roots, stems, leaves, flowers, and fruits of seabuckthorn, especially the fruit of seabuckthorn, are rich in nutrients and biologically active substances, and can be widely used in many fields of the national economy such as food, medicine, light industry, aerospace, agriculture, animal husbandry and fishery. Seabuckthorn fruit used as medicine has the effects of relieving cough and reducing phlegm, invigorating stomach and eliminating food, promoting blood circulation and dispelling blood stasis. Accord...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16
CPCC07K1/16C07K1/34C07K1/36C12P21/06
Inventor 陈雨诗
Owner 磐安县派普特生物科技有限公司
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