Separation component d of ethyl acetate extract of Sargassum heinzii and its preparation method and application in marine fouling biological control

A technology of ethyl acetate extraction and Sargassum Heinzii, applied in botany equipment and methods, chemicals for biological control, applications, etc., to achieve simple separation and preparation, good social benefits, and the effect of inhibiting marine organisms from attaching

Inactive Publication Date: 2020-12-08
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report on whether Sargassum henzii contains active ingredients against larvae attachment and spore germination of marine fouling organisms and whether it can be applied to the field of marine fouling biocontrol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Sargassum Heinzki ethyl acetate extract separation component D is extracted from Sargassum Heinzki, and its separation and extraction steps include:

[0023] (1) Sargassum Heinzii was extracted 3 times with a 95% ethanol aqueous solution by volume fraction to obtain an ethanol extract, which was concentrated by vacuum rotary evaporation at 45° C., and ethanol was removed to obtain a paste extract;

[0024] (2) The paste extract was resuspended in water at a ratio of 6 ml per gram, extracted 3 times with an equal volume of petroleum ether, discarded the petroleum ether extraction components, and extracted the remaining water layer with an equal volume of ethyl acetate for 3 times, the combined ethyl acetate extract was concentrated and dried to obtain the ethyl acetate extract;

[0025] (3) Carry out chromatographic separation of the ethyl acetate extract in an atmospheric silica gel column (silica gel 8000g, column length 180cm, column diameter 20cm), with sherwood oil-...

Embodiment 2

[0028] Experimental group: Dissolve component D of the ethyl acetate extract of Sargassum heinzii in a mixed solvent of chloroform and methanol (the mixed solvent of chloroform and methanol is formed by mixing chloroform and methanol at a volume ratio of 3:1), and the prepared concentration is 282.6 μg / ml The solution. Add 1 ml of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the dose of fraction D of the ethyl acetate extract of Sargassum heinzii coated on the bottom of the petri dish is 10 μg / cm 2 . Then 13ml of seawater was added to the Petri dish.

[0029] Control group: add 1ml of chloroform-methanol mixed solvent (chloroform-methanol mixed solvent is a mixture of chloroform and methanol at a volume ratio of 3:1), and distribute it evenly on the bottom of the petri dish, and then add 13ml of seawater after volatilization is complete.

[0030] Blank group: add 13m...

Embodiment 3

[0036] Experimental group: Dissolve component D of the ethyl acetate extract of Sargassum heinzii in a mixed solvent of chloroform and methanol (the mixed solvent of chloroform and methanol is formed by mixing chloroform and methanol at a volume ratio of 3:1), and the prepared concentration is 282.6 μg / ml The solution. Add 1 ml of this solution to a petri dish with a diameter of 6 cm and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the dose of fraction D of the ethyl acetate extract of Sargassum Heinzii coated on the bottom of the petri dish is 10 μg / cm 2 . Then 13ml of seawater was added to the Petri dish.

[0037] Control group: Add 1ml of chloroform-methanol mixed solvent (chloroform-methanol mixed solvent is a mixture of chloroform and methanol at a volume ratio of 3:1), so that the solvent is evenly distributed on the bottom of the petri dish, and after the solvent is completely evaporated, add 13ml of seawater.

[0038...

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PUM

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Abstract

The invention discloses a sargassum henslouianum ethyl acetate extract separation component D as well as a preparation method and application thereof to prevention and removal of marine fouling organisms. When the sargassum henslouianum ethyl acetate extract separation component D disclosed by the invention is covered on the surface of a solid by a low dosage, the effect of inhibiting the attachment of acorn barnacle and mussel larvae is realized, so that the sargassum henslouianum ethyl acetate extract separation component D can be used for preparing a marine fouling organism prevention and removal agent. The sargassum henslouianum ethyl acetate extract separation component D is obtained by extracting from sargassum henslouianum and a natural organic compound, so that a water body environment is not polluted and enrichment of the sargassum henslouianum ethyl acetate extract separation component D in organisms is caused by food chain transferring; the sargassum henslouianum ethyl acetate extract separation component D is environmentally friendly and has high safety, is used for effectively inhibiting the attachment of the marine organisms and does not contain heavy metal elements including copper, tin, and the like; the sargassum henslouianum ethyl acetate extract separation component D has good social benefits from the aspect of environment protection and has a wide source; aseparation and preparation way is simple and convenient and is suitable for being produced in a large scale, so that the sargassum henslouianum ethyl acetate extract separation component D has a greatpopularization potential and has a good application prospect in the prevention and removal of the marine fouling organisms.

Description

Technical field: [0001] The invention belongs to the field of natural products, and in particular relates to the separation component D of ethyl acetate extract of Sargassum henzii and its preparation method and application in marine fouling biological control. Background technique: [0002] Marine fouling organisms refer to all kinds of organisms that fix or inhabit underwater parts of ships and artificial facilities and have adverse effects on human economic activities. The metal corrosion process causes corrosion and perforation; increases the dynamic load effect, causing man-made facilities to drift, unbalance or even overturn; competes with breeding objects for attachment substrates and bait, hinders their growth and development, and reduces the quality of aquatic products. Therefore, in the process of developing the marine economic industry, marine fouling biological control is always an indispensable and important part. [0003] Marine fouling organisms are composed ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/00A01N61/00A01P9/00C09D5/16
CPCA01N65/00C09D5/16
Inventor 卢虹玉严涛章超桦曹文浩吴慧林明晴谢恩义
Owner GUANGDONG OCEAN UNIVERSITY
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