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Biomarker for forecasting esophageal squamous cell carcinoma prognosis

A technology of biomarkers and prognostic markers, applied in the application of biomarkers to predict the prognosis of esophageal squamous cell carcinoma, and in the field of biomarkers to predict the prognosis of esophageal squamous cell carcinoma, can solve the problems that did not take into account esophageal squamous cell carcinoma , to achieve the effect of increasing the degree of certainty

Active Publication Date: 2018-07-24
JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is simple and easy to use, but the disadvantage is that it does not take into account the influence caused by the differences in molecular mechanisms of esophageal squamous cell carcinoma itself

Method used

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  • Biomarker for forecasting esophageal squamous cell carcinoma prognosis
  • Biomarker for forecasting esophageal squamous cell carcinoma prognosis
  • Biomarker for forecasting esophageal squamous cell carcinoma prognosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1 Screening of prognostic markers based on public big data

[0067] see figure 1 As shown, based on the exome sequencing data of 113 pairs of esophageal squamous cell carcinoma and paracancerous tissue DNA samples published earlier in the Chinese Han population (Gao YB, et al. Genetic landscape of esophageal squamouscell carcinoma. Nature genetics 2014;46: 1097 -1102.), the research analysis found that 87 pairs of samples contained a total of 3145 DNA copy number variation information. Using the GENCOD V19 gene annotation tool (http: / / www.gencodegenes.org / ) to confirm the copy number variation information of these genes, it was found that 1728 genes with protein coding function showed copy number variation, of which 76 genes were in Copy number variants (high frequency variants) found in three or more tissue samples.

[0068] Then we further retrieved the gene chip data information of the same cohort of patients with esophageal squamous cell carcinoma (GEO dat...

Embodiment 2

[0071] Example 2 Validation of Prognostic Markers

[0072] 1. Fabrication of Tissue Chips

[0073] 197 cases and 118 cases of esophageal squamous cell carcinoma patients and adjacent tissues were extracted from the Chinese Academy of Medical Sciences Cancer Hospital and the First Affiliated Hospital of Nanjing Medical University, respectively. There are complete clinical and pathological data and prognosis follow-up data. All tissue samples were firstly stained with hematoxylin-eosin after paraffin embedding to confirm that the paraffin tissue block was esophageal squamous cell carcinoma or paracancerous tissue, and then sliced ​​to select a representative tumor area (cancer cells>80%) or Paracancerous area (0 for cancer cells). For each specimen, cancerous tissue or paracancerous tissue was selected twice, and samples from the two centers were placed on different tissue chips.

[0074] 2. Hematoxylin-eosin staining, including the following steps:

[0075] a. Place the tis...

Embodiment 3

[0109] Example 3 Efficacy detection of prognostic markers - high expression of ANO1 promotes proliferation, apoptosis resistance, invasion and migration of esophageal squamous cell carcinoma cells

[0110] a. Cell culture

[0111]Human esophageal squamous cell carcinoma cell lines KYSE30, KYSE70, KYSE140, KYSE180, KYSE410, ECA109, TE-1 and normal esophageal epithelial cells HET-1a were purchased from Shanghai Jikai Biological Company. HET-1a cells were cultured in DMEM medium, other cancer cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum and 1% penicillin / streptomycin, the culture temperature was 37°C, and the carbon dioxide concentration in the culture environment was 5%, of which DMEM medium and RPMI-1640 medium were purchased from Gibco, USA.

[0112] b. RNA extraction and PCR detection

[0113] According to the requirements of the kit instructions, the total RNA extraction kit SuperfecTRI was used to extract RNA from the cells. The extracted tot...

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Abstract

The invention discloses a biomarker for forecasting esophageal squamous cell carcinoma prognosis. A forecasting method comprises the following steps of screening of prognostic markers based on publiclarge data, verification of the prognostic markers and efficacy detection of the prognostic markers. The study discovers and verifies for the first time that an ANO1 binding MMP3 protein can be used as the biomarker for forecasting esophageal squamous cell carcinoma prognosis, the forecasting efficacy of the ANO1 binding MMP3 protein is equal to the forecasting efficacy of the eighth edition of TNM staging of clinically common prognosis forecasting by purely utilizing clinic information at present, and the accuracy of prognosis forecasting of a patient with esophageal squamous cell carcinoma can be remarkably improved with combination use of the two methods. At the same time, the study discovers that an ANO1 gene highly expresses in cancer tissues of the patient with the esophageal squamous cell carcinoma, expression increase of the ANO1 gene can promote proliferation, invasion and metastasis of the esophageal squamous cell carcinoma, and in vivo and in vitro progresses of the esophageal squamous cell carcinoma can be remarkably inhibited by utilizing expression of an shRNA knockout ANO1 gene.

Description

technical field [0001] The invention relates to a biomarker for predicting prognosis of esophageal squamous cell carcinoma, in particular to a biomarker for predicting prognosis of esophageal squamous cell carcinoma, and belongs to the application field of biomarkers for predicting prognosis of esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer is one of the most common malignant tumors. Worldwide, the incidence of esophageal cancer ranks eighth among all malignant tumors, and the death rate ranks sixth. About 45,000 people worldwide suffer from this disease every year. Unlike Western countries, which are mostly esophageal adenocarcinoma, esophageal squamous cell carcinoma (esophageal squamous cell carcinoma) accounts for more than 95% of the overall incidence of esophageal cancer in my country. The current main treatment methods for esophageal squamous cell carcinoma are surgery, radiotherapy and chemotherapy. Although the comprehensive tre...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574G06F19/18
CPCC12Q1/6886C12Q2600/118C12Q2600/158G01N33/57407G01N33/57492G01N33/57496G01N2333/47G16B20/00
Inventor 许晶于跃吴卫兵陈亮朱全阙军王俊闻伟潘相龙徐心峰曹靖
Owner JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
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