Preparation and application of chemiluminescent immunoprobe based on dendrimer double-amplification labeling

A chemiluminescent immunity and double amplification technology, which is applied in chemiluminescence/bioluminescence, analysis through chemical reactions of materials, scientific instruments, etc., can solve the problems of not being able to make full use of the amplification modes of biotin and avidin, and being helpless. Achieve the effect of improving the intensity of the luminous signal and enhancing the performance
CN108344864AInactive Publication Date: 2018-07-31NANJING TZONE BIOLOGICAL SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING TZONE BIOLOGICAL SCI & TECH
Publication Date
2018-07-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a preparation and application of a chemiluminescent immunoprobe based on dendrimer double-amplification labeling, and belongs to the field of chemiluminescent labeling in chemiluminescence in-vitro diagnosis. The preparation method of the amplified chemiluminescence immunodetection probe comprises the following steps: 1, preparation of a dendrimer-streptavidin-chemiluminescent signal substance complex; 2, biotinylation labeling of an antibody; and 3, preparation of a dual-amplification chemiluminescent immunolabeling detection probe complex. According to the dual-amplifying chemiluminescence immunodetection probe, the mode of enhancing the fluorescent intensity and the mode of increasing the detecting molecule combining weight of fluorescent microspheres are effectively combined, one-time amplification is achieved through the combination effect between biotin and avidin, second-time amplification is achieved by use of a signal-substance-labeled dendrimer, and the signal intensity of chemiluminescence immunoassay is greatly improved through the dual amplifying mode.
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Description

technical field

[0001] The invention belongs to the field of chemiluminescence labeling in chemiluminescence in vitro diagnosis, and in particular relates to a preparation method and application of a double amplification chemiluminescence immunolabeling probe. Background technique

[0002] Immunoassay technology is currently one of the most basic and commonly used detection methods in the fields of medicine, biology, food safety and other fields. This technology is based on the principle of specific reaction between antibodies and antigens for qualitative and quantitative analysis of the test object, and is widely used in medical biology. In basic research, clinical medicine, environmental monitoring, disease diagnosis, disease development prediction and even detection of food and microbial contamination.

[0003] Chemiluminescence Immunoassay (CLIA) is a combination of high-sensitivity chemiluminescence assay technology and high-specificity immune response for various antig...

Claims

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