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Method for preparing xylooligosaccharide through H2O2-HOAc coupling pre-processing

A technology of xylo-oligosaccharide and h2o2-hoac, applied in the direction of fermentation, can solve the problems of hindering the enzymatic hydrolysis of xylan, low extraction rate of pretreatment method, affecting the yield of xylo-oligosaccharide, etc. Environmental pollution, lower raw material pretreatment temperature, easy separation effect

Inactive Publication Date: 2018-08-03
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, xylan and lignin in bagasse exist in the form of "xylan-lignin" complexes, which become "lignin barriers" and hinder the enzymatic hydrolysis of xylan, so the existing process is Before the preparation of xylo-oligosaccharides, xylan must be extracted from the raw material bagasse or the raw material bagasse must be effectively pretreated
At present, some studies use the "alkaline extraction" method to extract xylan from the raw material bagasse. However, the extraction rate of this pretreatment method is low, which affects the yield of subsequent enzymatic preparation of xylo-oligosaccharides.

Method used

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  • Method for preparing xylooligosaccharide through H2O2-HOAc coupling pre-processing
  • Method for preparing xylooligosaccharide through H2O2-HOAc coupling pre-processing
  • Method for preparing xylooligosaccharide through H2O2-HOAc coupling pre-processing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 A kind of H 2 O 2 Method for preparing xylo-oligosaccharide by -HOAc coupling pretreatment

[0038] This embodiment provides a H 2 O 2 -The method for preparing xylo-oligosaccharides with HOAc coupling pretreatment, including:

[0039] (1) Take 5g (absolutely dry) pulverized and sieved bagasse of 40 meshes, placed in a 200mL polytetrafluoroethylene reaction tank, followed by H 2 O 2 (21.6 mol / L): HOAc (8.74 mol / L)=1:1 to adjust the solid-liquid ratio to 1:20.

[0040] After the polytetrafluoroethylene reaction tank was sealed, it was placed in a homogeneous reaction kettle, and the pretreatment temperature was heated to the required reaction temperature of 60°C at a speed of 5°C / min, and the reaction was carried out at a turnover speed of 10rpm for 3h.

[0041] After the pretreatment, the solid residue was washed to neutrality and dried at 50°C to directly enter the stage of alkaline extraction of xylan.

[0042] (2) Take 3 g of drying raw materials and...

Embodiment 2

[0045] Example 2 A kind of H 2 O 2Method for preparing xylo-oligosaccharide by -HOAc coupling pretreatment

[0046] This embodiment provides a H 2 O 2 -The method for preparing xylo-oligosaccharides with HOAc coupling pretreatment, including:

[0047] (1) Take 5g (absolutely dry) pulverized and sieved bagasse of 40 meshes, placed in a 200mL polytetrafluoroethylene reaction tank, followed by H 2 O 2 (21.6 mol / L): HOAc (8.74 mol / L)=1:1 to adjust the solid-liquid ratio to 1:20.

[0048] After the polytetrafluoroethylene reaction tank was sealed, it was placed in a homogeneous reaction kettle, and the pretreatment temperature was heated to the required reaction temperature of 80°C at a speed of 5°C / min, and the reaction was carried out at a turnover speed of 10rpm for 3h.

[0049] After the pretreatment, the solid residue was washed to neutrality and dried at 50°C to directly enter the stage of alkaline extraction of xylan.

[0050] (2) Take 3 g of drying raw materials and ...

Embodiment 3

[0054] Example 3 A kind of H 2 O 2 Method for preparing xylo-oligosaccharide by -HOAc coupling pretreatment

[0055] This embodiment provides a H 2 O 2 -The method for preparing xylo-oligosaccharides with HOAc coupling pretreatment, including:

[0056] (1) Take 5g (absolutely dry) pulverized and sieved bagasse of 40 meshes, placed in a 200mL polytetrafluoroethylene reaction tank, followed by H 2 O 2 (21.6 mol / L): HOAc (8.74 mol / L)=1:1 to adjust the solid-liquid ratio to 1:20.

[0057] After sealing the polytetrafluoroethylene reaction tank, put it in a homogeneous reaction kettle, heat the pretreatment temperature to the desired reaction temperature of 100°C at a speed of 5°C / min, and react at a turnover speed of 10rpm for 3h.

[0058] After the pretreatment, the solid residue was washed to neutrality and dried at 50°C to directly enter the stage of alkaline extraction of xylan.

[0059] (2) Take 3 g of drying raw materials and put it in a 500 mL glass beaker. The dosag...

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Abstract

The invention relates to a method for preparing xylooligosaccharide through H2O2-HOAc coupling pre-processing. The method comprises the following steps: (1) conducting H2O2-HOAc pre-processing on bagasse, and conducting filtering, so that pre-processed filtrate and filter residues are obtained; (2) conducting alkaline extraction and alcohol precipitation on the obtained filter residues, so that xylan is obtained; and (3) enzymatically hydrolyzing the xylan, so that the xylooligosaccharide is obtained. According to the method provided by the invention, H2O2-HOAc pre-processing is conducted on the bagasse, so that a hemicellulose xylan-lignin composite structure is broken, lignin is effectively removed and the efficiency of preparing the xylooligosaccharide through a method of implementing alkaline extraction on hemicellulose xylan is improved; and through the extraction process, sufficient xylan extraction is achieved, a quite low amount of xylose, which is generated from degradation, is guaranteed, and equipment corrosion and environmental pollution are relieved; therefore, the method provided by the invention is high in efficiency, low in cost and free from pollution.

Description

technical field [0001] The present invention relates to a H 2 O 2 The method for preparing xylo-oligosaccharide by -HOAc coupling pretreatment belongs to the field of biomass chemistry and chemical industry. Background technique [0002] In the sugarcane sugar industry, the sources of bagasse are relatively concentrated and the output is huge. According to the quality, type and pressing conditions of sugarcane, 2 tons of bagasse will be produced for every 1 ton of sucrose produced. At present, the annual output of sugarcane in my country is about 85 million tons, that is, the annual output of bagasse is about 22 million tons. The treatment and comprehensive utilization of such a huge amount of bagasse is an important issue in the development of the sugar cane industry. [0003] The tissue structure of bagasse is a unique supramolecular morphological structure, which takes the crystalline microfibrils of cellulose as the skeleton, and the hemicellulose and lignin form a fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/00C12P19/12
CPCC12P19/00C12P19/12C12P19/14
Inventor 蒋建新游艳芝李鹏飞王堃
Owner BEIJING FORESTRY UNIVERSITY
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