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A method for changing the color development of genipin cross-linked collagen by using sunlight

A sunlight-linked collagen technology, applied in prosthetics, medical science, etc., can solve the problems of uncontrollable time, reduce the degree of cross-linking, slow down, etc., and achieve safe processing methods, good biocompatibility, and low biological toxicity Effect

Active Publication Date: 2020-08-04
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pigment has good stability, has fluorescence effect, is convenient for medical observation, and will slowly oxidize and fade. However, due to the low visual acceptance of the color, genipin cross-linked products are rarely used in surgical operations.
[0004] In order to improve the deposition of blue melanin, the method of reducing the degree of cross-linking is usually used, but it is not good for improving the mechanical strength and degradation resistance of cross-linked products
If the pigment itself oxidizes and fades, the time is uncontrollable, and there is no guarantee that it can meet the appearance and use requirements of cross-linked products
At present, there is no effective way to eliminate the blue melanin associated with the cross-linking process. This color reaction is mostly used for wound repair surgery marks or tattoos. effect

Method used

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  • A method for changing the color development of genipin cross-linked collagen by using sunlight
  • A method for changing the color development of genipin cross-linked collagen by using sunlight
  • A method for changing the color development of genipin cross-linked collagen by using sunlight

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Comparison scheme
Effect test

Embodiment 1

[0027] A method utilizing sunlight to change the color development of genipin cross-linked collagen, comprising the following steps:

[0028] (1) Under the stirring state, in the acidic collagen solution of 7 mg / ml, the acidic titania nanocrystalline hydrosol of 0.1 mol / L is slowly dripped at 5% by volume;

[0029] (2) Adjust the pH value of the mixed solution to 6-8, pour it into a 6-well plate (6ml / well) and place it in a water bath at 35°C for 12 hours to solidify into a gel;

[0030] (3) The gel was soaked in 6ml of genipin solution with a mass fraction of 1% and cross-linked for 12 hours, and then appeared blue-black; then washed with pure water until no genipin solution remained on the collagen surface;

[0031] (4) The blue-black gel was originally exposed to natural light in summer for 6 hours (10:00-16:00), and the color of the gel changed to light blue-gray.

Embodiment 2

[0033] A method utilizing sunlight to change the color development of genipin cross-linked collagen, comprising the following steps:

[0034] (1) Under the stirring state, in the acidic collagen solution of 7 mg / ml, the acidic titania nanocrystalline hydrosol of 0.1 mol / L is slowly dripped at 5% by volume;

[0035] (2) Adjust the pH value of the mixed solution to 6-8, pour it into a 6-well plate (6ml / well) and place it in a water bath at 35°C for 12 hours to solidify into a gel;

[0036] (3) The gel was soaked in 6ml of genipin solution with a mass fraction of 1% and cross-linked for 12 hours, and then appeared blue-black; then washed with pure water until no genipin solution remained on the surface;

[0037] (4) After 12 hours of natural light in summer (7:00-19:00), the color of the blue-black gel becomes light brown.

Embodiment 3

[0039] A method utilizing sunlight to change the color development of genipin cross-linked collagen, comprising the following steps:

[0040] (1) Under the stirring state, in the acidic collagen solution of 5 mg / ml, the acidic titania nanocrystalline hydrosol of 0.1 mol / L is slowly dripped at 3% by volume;

[0041] (2) Adjust the pH value of the mixed solution to 6-8, pour it into a 6-well plate (6ml / well) and place it in a water bath at 35°C for 12 hours to solidify into a gel;

[0042] (3) The gel was soaked in 6ml of genipin solution with a mass fraction of 1% and cross-linked for 12 hours, and then appeared blue-black; then washed with pure water until no genipin solution remained on the surface;

[0043] (4) The blue-black gel is simulated by the sun light source (the irradiance of the simulated sun light source is 1000W / m 2 , UV irradiance 30W / cm 2 ) light for 6 hours, the color of the gel changed to light brown.

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Abstract

The invention discloses a method for changing genipin cross-linking collagen color development by sunlight. The method includes that genipin cross-linking collagen is exposed to sunlight or simulatedsunlight for 4-12h; preferably, the genipin cross-linking collagen is exposed to sunlight or simulated sunlight for 12h to fade into milk white. A genipin cross-linking product associated pigment treatment method is safe, convenient and high in biocompatibility. By making full use of the advantage of low genipin cross-linking biotoxicity, the problem of color development of associated pigments issolved while structural stability is improved, and a research and development direction is provided for adopting visible light for preparing collagen based medical auxiliary materials, artificial skinand the like suitable for different complexions. By a light irradiation treatment method, on the one hand, a material basis is provided for quick fading of cross-linking product associated pigments under the condition of natural light irradiation, and on the other hand, simulated sunlight source testing conditions are provided for directly preparing genipin cross-linking products in different colors.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a method for changing the color development of genipin crosslinked collagen by using sunlight. Background technique [0002] Natural natural polymer materials such as collagen and gelatin are widely used in tissue engineering scaffolds, artificial skin, biomedical implants, wound dressings and other fields due to their good histocompatibility and degradability, but due to their insufficient mechanical strength , the spatial configuration cannot be maintained during the hydrolysis process in vivo, and the absorption is too fast. Therefore, components such as chitosan are often added in application to improve the elastic modulus, critical deformation and strength of the scaffold. Cross-linking can make intra-molecular and inter-molecular bonding through covalent bonds, thereby enhancing the structural stability and mechanical strength of the polymer network and the ability to re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/28C08J3/24C08L89/00A61L27/60A61L27/50A61L27/24
CPCA61L27/24A61L27/50A61L27/60C08J3/24C08J3/28C08J2389/00C08L89/00
Inventor 张玲张浩然唐笑刘晓燕
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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